Difference between revisions of "PMID:7635817"

From omp dev
Jump to: navigation, search
(New PMID: Page!)
 
(Table edited by Wameza via TableEdit)
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
 +
{{RightTOC}}
  
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.3998.T515f50e825eee-->
 +
<!--
 +
******************************************************************************************
 +
*
 +
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
 +
*
 +
****************************************************************************************** -->
 +
{|  id="T515f50e825eee"  class=" tableEdit PMID_info_table" 
 +
 +
|-
 +
!align=left  |Citation
 +
||
 +
'''Thöny-Meyer, L, Fischer, F, Künzler, P, Ritz, D and Hennecke, H'''  (1995) Escherichia coli genes required for cytochrome c maturation. ''J. Bacteriol.'' '''177''':4321-6
 +
|-
 +
!align=left  |Abstract
 +
||
 +
The so-called aeg-46.5 region of Escherichia coli contains genes whose expression is induced under anaerobic growth conditions in the presence of nitrate or nitrite as the terminal electron acceptor. In this work, we have examined more closely several genes of this cluster, here designated ccmABCDEFGH, that are homologous to two separate Bradyrhizobium japonicum gene clusters required for the biogenesis of c-type cytochromes. A deletion mutant of E. coli which lacked all of these genes was constructed. Maturation of indigenous c-type cytochromes synthesized under anaerobic respiratory conditions, with nitrite, nitrate, or trimethylamine N-oxide as the electron acceptor, was found to be defective in the mutant. The biogenesis of foreign cytochromes, such as the soluble B. japonicum cytochrome c550 and the membrane-bound Bacillus subtilis cytochrome c550, was also investigated. None of these cytochromes was synthesized in its mature form when expressed in the mutant, as opposed to the situation in the wild type. The results suggest that the E. coli ccm gene cluster present in the aeg-46.5 region is required for a general pathway involved in cytochrome c maturation.
 +
|-
 +
!align=left  |Links
 +
||
 +
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7635817 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC177179 PMC177179]
 +
 +
|-
 +
!align=left  |Keywords
 +
||
 +
Anaerobiosis; Bacillus subtilis/genetics; Bacillus subtilis/metabolism; Cytochrome c Group/biosynthesis; Cytochrome c Group/genetics; Cytochrome c Group/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Deletion; Gene Expression Regulation, Bacterial; Genes, Bacterial; Methylamines/metabolism; Molecular Sequence Data; Mutation; Nitrates/metabolism; Nitrites/metabolism; Oxidants/metabolism; Rhizobiaceae/genetics; Rhizobiaceae/metabolism; Sequence Homology
 +
 +
|- class="tableEdit_footer"
 +
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3998.T515f50e825eee&page=3998&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> ||
 +
|}
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.3998.T515f50e825eee-->
 +
 +
==Main Points of the Paper ==
 +
{{LitSignificance}}
 +
 +
== Materials and Methods Used ==
 +
{{LitMaterials}}
 +
 +
==Phenotype Annotations==
 +
{{AnnotationTableHelp}}
 +
<protect><!--box uid=d41d8cd98f00b204e9800998ecf8427e.3998.A515f50e861635-->
 +
<!--
 +
******************************************************************************************
 +
*
 +
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
 +
*
 +
****************************************************************************************** -->
 +
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="A515f50e861635"  class=" tableEdit Phenotype_Table_2" 
 +
|-
 +
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: MC1061
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''ccmABCDEFG<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''ccmABCDEFG(del)''
 +
|
 +
*Reference Condition:
 +
|
 +
 +
|
 +
 +
|
 +
0000178
 +
|
 +
in vivo assay evidence
 +
|
 +
Loss of heme-containing membrane proteins. See figure 3 for experimental results.
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: MC1061
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''ccmABCDEFG<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''ccmABCDEFG(del)'' in EC06
 +
|
 +
*Reference Condition:
 +
|
 +
 +
|
 +
 +
|
 +
0000178
 +
|
 +
in vivo assay evidence
 +
|
 +
Loss of mature cytochrome<sub>c550</sub>
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: MC1061
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''ccmABCDEFG<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''ccmABCDEFG(del)'' in EC06
 +
|
 +
*Reference Condition:
 +
|
 +
 +
|
 +
 +
|
 +
0000178
 +
|
 +
in vivo assay evidence
 +
|
 +
deficient cytochrome c bio-genesis.
 +
|
 +
 +
 +
|- class="tableEdit_footer"
 +
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3998.A515f50e861635&page=3998&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</span> || || || || || || || || ||
 +
|}
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.3998.A515f50e861635--></protect>
 +
 +
==Notes==
 +
 +
==References==
 +
{{RefHelp}}
 +
<references/>
 +
 +
 +
[[Category:Publication]]

Latest revision as of 14:07, 9 April 2013

Citation

Thöny-Meyer, L, Fischer, F, Künzler, P, Ritz, D and Hennecke, H (1995) Escherichia coli genes required for cytochrome c maturation. J. Bacteriol. 177:4321-6

Abstract

The so-called aeg-46.5 region of Escherichia coli contains genes whose expression is induced under anaerobic growth conditions in the presence of nitrate or nitrite as the terminal electron acceptor. In this work, we have examined more closely several genes of this cluster, here designated ccmABCDEFGH, that are homologous to two separate Bradyrhizobium japonicum gene clusters required for the biogenesis of c-type cytochromes. A deletion mutant of E. coli which lacked all of these genes was constructed. Maturation of indigenous c-type cytochromes synthesized under anaerobic respiratory conditions, with nitrite, nitrate, or trimethylamine N-oxide as the electron acceptor, was found to be defective in the mutant. The biogenesis of foreign cytochromes, such as the soluble B. japonicum cytochrome c550 and the membrane-bound Bacillus subtilis cytochrome c550, was also investigated. None of these cytochromes was synthesized in its mature form when expressed in the mutant, as opposed to the situation in the wild type. The results suggest that the E. coli ccm gene cluster present in the aeg-46.5 region is required for a general pathway involved in cytochrome c maturation.

Links

PubMed PMC177179

Keywords

Anaerobiosis; Bacillus subtilis/genetics; Bacillus subtilis/metabolism; Cytochrome c Group/biosynthesis; Cytochrome c Group/genetics; Cytochrome c Group/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Deletion; Gene Expression Regulation, Bacterial; Genes, Bacterial; Methylamines/metabolism; Molecular Sequence Data; Mutation; Nitrates/metabolism; Nitrites/metabolism; Oxidants/metabolism; Rhizobiaceae/genetics; Rhizobiaceae/metabolism; Sequence Homology

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: MC1061
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ccmABCDEFG+
  • Genotype of Experimental Strain : ccmABCDEFG(del)
  • Reference Condition:

0000178

in vivo assay evidence

Loss of heme-containing membrane proteins. See figure 3 for experimental results.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: MC1061
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ccmABCDEFG+
  • Genotype of Experimental Strain : ccmABCDEFG(del) in EC06
  • Reference Condition:

0000178

in vivo assay evidence

Loss of mature cytochromec550

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: MC1061
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ccmABCDEFG+
  • Genotype of Experimental Strain : ccmABCDEFG(del) in EC06
  • Reference Condition:

0000178

in vivo assay evidence

deficient cytochrome c bio-genesis.


edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

Retrieved from "http://bcbp-ftq4xm2.biobio.tamu.edu/omp/dev/index.php?title=PMID:7635817&oldid=10294"