PMID:7007354

Citation	Schaus, N, O'Day, K, Peters, W and Wright, A (1981) Isolation and characterization of amber mutations in gene dnaA of escherichia coli K-12. J. Bacteriol. 145:904-13
Abstract	Amber mutants with defects in the dnaA gene of Escherichia coli K-12 were isolated after localized mutagenesis of the tna-dnaA region of the chromosome. We isolated 36 mutants defective in the initiation of deoxyribonucleic acid replication as determined by their dependence upon integrative suppression by a P2 sig5 prophage. Three of the 36 mutants were shown to contain amber mutations through the use of a temperature-sensitive amber suppressor. These mutations, which mapped between gyrB and tna, were characterized genetically and biochemically as amber mutations in dnaA.
Links	PubMed PMC217197
Keywords	Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Chromosome Mapping; DNA Replication; DNA, Bacterial/genetics; Escherichia coli/genetics; Genes; Genetic Complementation Test; Mutation; Transduction, Genetic
edit table

Main Points of the Paper

Please summarize the main points of the paper.

• 3 amber mutations in dnaA were isolated and characterized

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

• Conditional

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
edit table

</protect>

References

See Help:References for how to manage references in omp dev.