Difference between revisions of "PMID:7007354"
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* P1 transduction | * P1 transduction | ||
* Localized mutagenesis of the ''dnaA'' region (described elsewhere) | * Localized mutagenesis of the ''dnaA'' region (described elsewhere) | ||
| − | *lambda<sup>21</sup> | + | *lambda imm<sup>21</sup> Spi<sup>-</sup> ''dnaA'' specialized transducing phage |
==Phenotype Annotations== | ==Phenotype Annotations== | ||
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*Genotype of Experimental Strain : ''dnaA311(Am)'' | *Genotype of Experimental Strain : ''dnaA311(Am)'' | ||
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| − | *Reference Condition: | + | *Reference Condition: 30<sup>o</sup> C |
| + | *Experimental Condition: 42<sup>o</sup> C | ||
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0000271 | 0000271 | ||
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*Genotype of Experimental Strain : ''dnaA366(Am)'' | *Genotype of Experimental Strain : ''dnaA366(Am)'' | ||
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| − | *Reference Condition: | + | *Reference Condition: 30<sup>o</sup> C |
| + | *Experimental Condition: 42<sup>o</sup> C | ||
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0000271 | 0000271 | ||
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*Genotype of Experimental Strain : ''dnaA367(Am)'' | *Genotype of Experimental Strain : ''dnaA367(Am)'' | ||
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| − | *Reference Condition: | + | *Reference Condition: 30<sup>o</sup>C |
| + | *Experimental Condition: 42<sup>o</sup>C | ||
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0000271 | 0000271 | ||
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==Notes== | ==Notes== | ||
| + | * temperature-dependent growth is a place holder for a new terms for Growth | ||
==References== | ==References== | ||
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[[Category:Publication]] | [[Category:Publication]] | ||
[[Category:Integrative Suppression]] | [[Category:Integrative Suppression]] | ||
| + | [[Category:LaRossa citations that need review]] | ||
Latest revision as of 18:38, 22 May 2013
| Citation |
Schaus, N, O'Day, K, Peters, W and Wright, A (1981) Isolation and characterization of amber mutations in gene dnaA of escherichia coli K-12. J. Bacteriol. 145:904-13 |
|---|---|
| Abstract |
Amber mutants with defects in the dnaA gene of Escherichia coli K-12 were isolated after localized mutagenesis of the tna-dnaA region of the chromosome. We isolated 36 mutants defective in the initiation of deoxyribonucleic acid replication as determined by their dependence upon integrative suppression by a P2 sig5 prophage. Three of the 36 mutants were shown to contain amber mutations through the use of a temperature-sensitive amber suppressor. These mutations, which mapped between gyrB and tna, were characterized genetically and biochemically as amber mutations in dnaA. |
| Links | |
| Keywords |
Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Chromosome Mapping; DNA Replication; DNA, Bacterial/genetics; Escherichia coli/genetics; Genes; Genetic Complementation Test; Mutation; Transduction, Genetic |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
- 3 amber mutations in dnaA were isolated and characterized
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
- Conditional Integrative Suppression
- This paper includes an extensive strain list
- P1 transduction
- Localized mutagenesis of the dnaA region (described elsewhere)
- lambda imm21 Spi- dnaA specialized transducing phage
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
|
a mutation or genetic difference within a strain |
|
|
|
0000271 |
temperature-dependent growth |
0000178 |
in vivo assay evidence |
reduced rate of DNA synthesis in high temperature. See figure 4A for experimental results. |
|
|
a mutation or genetic difference within a strain |
|
|
|
0000271 |
temperature-dependent growth |
0000178 |
in vivo assay evidence |
reduced rate of DNA synthesis in high temperature. See figure 4B for experimental results. |
|
|
a mutation or genetic difference within a strain |
|
|
|
0000271 |
temperature-dependent growth |
0000178 |
in vivo assay evidence |
reduced rate of DNA synthesis in high temperature. See figure 4C for experimental results. |
|
| edit table |
</protect>
Notes
- temperature-dependent growth is a place holder for a new terms for Growth
References
See Help:References for how to manage references in omp dev.
