Difference between revisions of "PMID:6094504"

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{|  id="Q51b24afcadd8c"  class=" tableEdit PMID_info_table" 
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!align=left  |Citation
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'''Fishel, RA and Kolodner, R'''  (1984) Escherichia coli strains containing mutations in the structural gene for topoisomerase I are recombination deficient. ''J. Bacteriol.'' '''160''':1168-70
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!align=left  |Abstract
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Mutations in the gene encoding topoisomerase I of Escherichia coli were tested for their effect on plasmid recombination. Recombination was decreased 1,000-fold at 30 and 37 degrees C and occurred at approximately wild-type frequencies at 42 degrees C. The suppression of topA mutations at 42 degrees C did not appear to be a result of increased topoisomerase I activity at 42 degrees C.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6094504 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC215837 PMC215837]
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!align=left  |Keywords
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==Main Points of the Paper ==
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{{LitSignificance}}
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== Materials and Methods Used ==
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{{LitMaterials}}
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==Phenotype Annotations==
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{{AnnotationTableHelp}}
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="M51b24afccf24e"  class=" tableEdit Phenotype_Table_2" 
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: DM700
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''topA<sup>+</sup>''
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*Genotype of Experimental Strain : SY791 ''top-10''
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*Reference Condition: 30<sup>o</sup>C
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*Experimental Condition: 30<sup>o</sup>C
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The mutation conferred a decrease in plasmid recombination. See Figure 2.
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|-
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|
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a mutation or genetic difference within a strain
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|
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: DM700
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''topA<sup>+</sup>''
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*Genotype of Experimental Strain : SY791 ''DE(topA-cysB)700''
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*Reference Condition: 30<sup>o</sup>C
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*Experimental Condition: 30<sup>o</sup>C
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|
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|
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|
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The mutation conferred a decrease in supper coiling. See Figure 2.
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|
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|-
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|
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a mutation or genetic difference within a strain
 +
|
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*Taxon: Escherichia coli
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*Strain: K-12
 +
*Substrain: DM700
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''topA<sup>+</sup>''
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*Genotype of Experimental Strain : SY791 ''top-10''
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|
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*Reference Condition: 30<sup>o</sup>C
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*Experimental Condition: 42<sup>o</sup>C
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|
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|
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|
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|
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The mutation conferred a decrease in plasmid recombination was negated at 42<sup>o</sup>C. See Figure 2.
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|
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 +
|-
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|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: DM700
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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|
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*Genotype of Reference Strain: ''topA<sup>+</sup>''
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*Genotype of Experimental Strain : SY791 ''DE(topA-cysB)700''
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|
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*Reference Condition: 30<sup>o</sup>C
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*Experimental Condition: 30<sup>o</sup>C
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|
 +
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
 +
The mutation conferred a decrease in plasmid recombination. See Figure 2.
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: DM700
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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|
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*Genotype of Reference Strain: ''topA<sup>+</sup>''
 +
*Genotype of Experimental Strain : SY791 ''DE(topA-cysB)700''
 +
|
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*Reference Condition: 30<sup>o</sup>C
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*Experimental Condition: 42<sup>o</sup>C
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|
 +
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
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The mutation conferred a decrease in plasmid recombination was negated at 42<sup>o</sup>C. See Figure 2.
 +
|
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 +
|-
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|
 +
a mutation or genetic difference within a strain
 +
|
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*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: DM700
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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|
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*Genotype of Reference Strain: ''topA<sup>+</sup>''
 +
*Genotype of Experimental Strain : SY791 ''top-10''
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|
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*Reference Condition: 30<sup>o</sup>C
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*Experimental Condition: 42<sup>o</sup>C
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
 +
The mutation which conferred a decrease in supper coiling was negated at 42<sup>o</sup>C. See Figure 2.
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: DM700
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''topA<sup>+</sup>''
 +
*Genotype of Experimental Strain : SY791 ''DE(topA-cysB)700''
 +
|
 +
*Reference Condition: 30<sup>o</sup>C
 +
*Experimental Condition: 30<sup>o</sup>C
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
 +
The mutation which conferred a decrease in supper coiling was negated at 42<sup>o</sup>C. See Figure 2.
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: DM700
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''topA<sup>+</sup>''
 +
*Genotype of Experimental Strain : SY791 ''top-10''
 +
|
 +
*Reference Condition: 30<sup>o</sup>C
 +
*Experimental Condition: 42<sup>o</sup>C
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
 +
The mutation conferred a decrease in supper coiling. See Figure 2.
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|
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 +
 +
|- class="tableEdit_footer"
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|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.4042.M51b24afccf24e&page=4042&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</span> || || || || || || || || ||
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==Notes==
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==References==
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{{RefHelp}}
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<references/>
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[[Category:Publication]]

Latest revision as of 17:32, 17 June 2013

Citation

Fishel, RA and Kolodner, R (1984) Escherichia coli strains containing mutations in the structural gene for topoisomerase I are recombination deficient. J. Bacteriol. 160:1168-70

Abstract

Mutations in the gene encoding topoisomerase I of Escherichia coli were tested for their effect on plasmid recombination. Recombination was decreased 1,000-fold at 30 and 37 degrees C and occurred at approximately wild-type frequencies at 42 degrees C. The suppression of topA mutations at 42 degrees C did not appear to be a result of increased topoisomerase I activity at 42 degrees C.

Links

PubMed PMC215837

Keywords


Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: DM700
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: topA+
  • Genotype of Experimental Strain : SY791 top-10
  • Reference Condition: 30oC
  • Experimental Condition: 30oC

The mutation conferred a decrease in plasmid recombination. See Figure 2.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: DM700
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: topA+
  • Genotype of Experimental Strain : SY791 DE(topA-cysB)700
  • Reference Condition: 30oC
  • Experimental Condition: 30oC

The mutation conferred a decrease in supper coiling. See Figure 2.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: DM700
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: topA+
  • Genotype of Experimental Strain : SY791 top-10
  • Reference Condition: 30oC
  • Experimental Condition: 42oC

The mutation conferred a decrease in plasmid recombination was negated at 42oC. See Figure 2.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: DM700
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: topA+
  • Genotype of Experimental Strain : SY791 DE(topA-cysB)700
  • Reference Condition: 30oC
  • Experimental Condition: 30oC

The mutation conferred a decrease in plasmid recombination. See Figure 2.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: DM700
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: topA+
  • Genotype of Experimental Strain : SY791 DE(topA-cysB)700
  • Reference Condition: 30oC
  • Experimental Condition: 42oC

The mutation conferred a decrease in plasmid recombination was negated at 42oC. See Figure 2.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: DM700
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: topA+
  • Genotype of Experimental Strain : SY791 top-10
  • Reference Condition: 30oC
  • Experimental Condition: 42oC

The mutation which conferred a decrease in supper coiling was negated at 42oC. See Figure 2.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: DM700
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: topA+
  • Genotype of Experimental Strain : SY791 DE(topA-cysB)700
  • Reference Condition: 30oC
  • Experimental Condition: 30oC

The mutation which conferred a decrease in supper coiling was negated at 42oC. See Figure 2.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: DM700
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: topA+
  • Genotype of Experimental Strain : SY791 top-10
  • Reference Condition: 30oC
  • Experimental Condition: 42oC

The mutation conferred a decrease in supper coiling. See Figure 2.


</protect>

Notes

References

See Help:References for how to manage references in omp dev.