Difference between revisions of "PMID:14168696"

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'''BECKWITH, JR'''  (1964) A DELETION ANALYSIS OF THE LAC OPERATOR REGION IN ESCHERICHIA COLI.''J. Mol. Biol.'' '''8''':427-30
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'''No author listed'''  (1964) A DELETION ANALYSIS OF THE LAC OPERATOR REGION IN ESCHERICHIA COLI. ''J. Mol. Biol.'' '''8''':427-30
 
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!align=left |Abstract
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No abstract in PubMed
 
No abstract in PubMed
 
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!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14168696 PubMed]
 
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14168696 PubMed]
  
 
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!align=left |Keywords
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Deletion; Lac; Operator; Escherichia coli; Galactosidases; Genetics; Membrane Transport Proteins; Mutation; RNA, Bacterial; Research; Enzymes; Revertants; Constitutive
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Enzymes; Escherichia coli; Galactosidases; Genetics; Membrane Transport Proteins; Mutation; RNA, Bacterial; Research
  
 
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==Main Points of the Paper ==
 
==Main Points of the Paper ==
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*The ''lac'' operator does not lie in the same region in which ''o<sup>o</sup>'' mutants are found.
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== Materials and Methods Used ==
 
== Materials and Methods Used ==
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*Mutant Classes
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**''o<sup>c</sup>'' mutants
 
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***lost the sensitivity to repressor, therefore expressing &beta;-galactosidase constitutively in the presence of the repressor
 
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*** very closely linked to ''z''- mutations mapped at the beginning of the ''z'' gene
 
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**''o<sup>o</sup>'' mutants
 
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***shuts off the production of all 3 enzymic activities of the ''lac'' operon
 
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*Types of ''z<sup>-</sup>y<sup>+</sup>'' revertants found in the melibiose-agar plate selection
 
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**Deletions within the ''lac'' region
 
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***abolish basal level of &beta;-galactosidase present in ''o<sup>o</sup>'' mutants
 
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***do not further revert to ''lac<sup>+</sup>'' phenotype
 
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**Mutations at unlinked suppressor loci
 
  
 
==Phenotype Annotations==
 
==Phenotype Annotations==
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!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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''Escherichia coli''
 
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NCBI:562
 
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''o<sup>o</sup>
 
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constitutive repression of all 3 enzymic activities of the ''lac'' operon
 
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Metabolic Activity
 
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Biochemical Assay
 
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''Escherichia coli''
 
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NCBI:562
 
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''o<sup>o</sup>2''-M116
 
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loss of repressibility
 
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Metabolic Activity
 
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Biochemical Assay
 
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Table 1
 
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''Escherichia coli''
 
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NCBI:562
 
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''o<sup>c</sup>
 
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constitutive expression of beta-galactosidase in the presence of repressor
 
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Metabolic Activity
 
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Biochemical Assay
 
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''Escherichia coli''
 
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NCBI:562
 
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''o<sup>o</sup>2''-M12
 
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no beta-galactosidase activity
 
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Metabolic Activity
 
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deletion in ''lac'' region
 
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Biochemical Assay
 
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Table 1
 
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''Escherichia coli''
 
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NCBI:562
 
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''o<sup>o</sup>2''-M15
 
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no beta-galactosidase activity
 
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Metabolic Activity
 
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deletion in ''lac'' region
 
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Biochemical Assay
 
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Table 1
 
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''Escherichia coli''
 
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NCBI:562
 
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''o<sup>o</sup>2''-M41
 
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no beta-galactosidase activity
 
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Metabolic Activity
 
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deletion in ''lac'' region
 
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Biochemical Assay
 
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Table 1
 
  
 
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==Notes==
 
==Notes==
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==References==
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<references/>
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[[Category:Publication]]

Latest revision as of 13:16, 1 September 2013

Citation

No author listed (1964) A DELETION ANALYSIS OF THE LAC OPERATOR REGION IN ESCHERICHIA COLI. J. Mol. Biol. 8:427-30

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Enzymes; Escherichia coli; Galactosidases; Genetics; Membrane Transport Proteins; Mutation; RNA, Bacterial; Research

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

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