Difference between revisions of "PMID:16591520"

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{|  id="I5223846d88b7f"  class=" tableEdit PMID_info_table"   
  
 
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!align=left |Citation
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'''Fox, CF, Carter, JR and Kennedy, EP'''  (1967) GENETIC CONTROL OF THE MEMBRANE PROTEIN COMPONENT OF THE LACTOSE TRANSPORT SYSTEM OF Escherichia coli.''Proc. Natl. Acad. Sci. U.S.A.'' '''57''':698-705
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'''No author listed'''  (1967) GENETIC CONTROL OF THE MEMBRANE PROTEIN COMPONENT OF THE LACTOSE TRANSPORT SYSTEM OF Escherichia coli. ''Proc. Natl. Acad. Sci. U.S.A.'' '''57''':698-705
 
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!align=left |Abstract
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No abstract in PubMed
 
No abstract in PubMed
 
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16591520 PubMed]
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16591520 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC335565 PMC335565]
  
 
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==Main Points of the Paper ==
 
==Main Points of the Paper ==
*The M protein of β-galactoside transport is the product of ''lacY''
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{{LitSignificance}}
*M is localized in the spheroplast membrane fraction of the cell
 
*Estimate: xx functional subunits of M protein /cell
 
  
 
== Materials and Methods Used ==
 
== Materials and Methods Used ==
*Strain: ML30  (''i<sup>+</sup>z<sup>+</sup>y<sup>+</sup>a<sup>+</sup>'')
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{{LitMaterials}}
*Strain: ML308-225 (''i<sup>-</sup>z<sup>-</sup>y<sup>+</sup>a<sup>+</sup>'')- a derivative of ML30
 
*Strain: 3000 (''i<sup>+</sup>z<sup>+</sup>y<sup>+</sup>a<sup>+</sup>'')
 
*Strain: YA470 (''i<sup>+</sup>z<sup>+</sup>y<sup>-</sup>'')- y<sup>-</sup> amber mutant
 
*Strain: CA7047 (''i<sup>+</sup>z<sup>+</sup>y<sup>-</sup>a<sup>+</sup>'')- y<sup>-</sup> amber mutant
 
*Strain: CA8202 (''i<sup>+</sup>z<sup>+</sup>y<sup>-</sup>a<sup>+</sup>'')- y<sup>-</sup> amber mutant
 
*Strain: CA8204 (''i<sup>+</sup>z<sup>+</sup>y<sup>-</sup>a<sup>+</sup>'')- y<sup>-</sup> amber mutant
 
*Strain: MS1054 (''i<sup>+</sup>z<sup>+</sup>y<sup>+</sup>a<sup>+</sup>'')- y<sup>-</sup> deletion
 
*Strain: MS356 (''i<sup>+</sup>z<sup>+</sup>y<sup>-</sup>a<sup>+</sup>'')- y<sup>-</sup> deletion
 
*Strain: MS1019 (''i<sup>+</sup>z<sup>+</sup>y<sup>-</sup>a<sup>+</sup>'')- y<sup>-</sup> deletion
 
*Strain: MS1068 (''i<sup>+</sup>z<sup>+</sup>y<sup>-</sup>a<sup>+</sup>'')- y<sup>-</sup> deletion
 
*Strain: A 279A (''i<sup>+</sup>z<sup>+</sup>y<sup></sup>a<sup>+</sup>'')
 
 
 
*Preparation of membrane-containing particulate fraction
 
*Unlabeled N-ethylmaleimide treatment
 
*M protein assay
 
  
 
==Phenotype Annotations==
 
==Phenotype Annotations==
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="X5223846d97d4e"  class=" tableEdit Phenotype_Table_2"   
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!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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''Escherichia coli''
 
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NCBI:562
 
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ML308
 
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''lacI<sup>-</sup>''
 
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*Abolished negative regulation of gene expression (LacY)
 
*Constitutive gene expression
 
 
 
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Other
 
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membrane particulate fractions contain the M protein regardless of induction status-constitutive expression of M protein (LacY)
 
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Other
 
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Table 1- protein-bound NEM
 
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''Escherichia coli''
 
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NCBI:562
 
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ML3
 
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''lacY<sup>-</sup>''
 
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*Absent substrate binding
 
*Absent transporter activity
 
*Abolished lactose catabolic process
 
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Other
 
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no detectable M protein in membrane particulate fractions
 
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Other
 
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Table 1- NEM binding 
 
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''Escherichia coli''
 
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NCBI:562
 
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ML35
 
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''lacY<sup>-</sup>''
 
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* Absent substrate binding
 
* Absent transporter activity
 
* Abolished lactose catabolic process
 
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Other
 
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no detectable M protein in membrane particulate fractions
 
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Other
 
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Table 1- NEM binding 
 
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''Escherichia coli'' K12
 
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NCBI:83333
 
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YA470
 
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''lacY<sup>-</sup>''
 
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* Absent substrate binding
 
* Absent transporter activity
 
* Abolished lactose catabolic process
 
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Other
 
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no detectable M protein in membrane particulate fractions
 
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Other
 
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Table 2- NEM binding 
 
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''Escherichia coli'' K12
 
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NCBI:83333
 
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CA7047
 
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''lacY<sup>-</sup>''
 
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* Absent substrate binding
 
* Absent transporter activity
 
* Abolished lactose catabolic process
 
|
 
Other
 
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no detectable M protein in membrane particulate fractions
 
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Other
 
|
 
Table 2- NEM binding 
 
|-
 
|
 
''Escherichia coli'' K12
 
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NCBI:83333
 
|
 
CA8202
 
|
 
''lacY<sup>-</sup>''
 
|
 
* Absent substrate binding
 
* Absent transporter activity
 
* Abolished lactose catabolic process
 
|
 
Other
 
|
 
no detectable M protein in membrane particulate fractions
 
|
 
Other
 
|
 
Table 2- NEM binding 
 
|-
 
|
 
''Escherichia coli'' K12
 
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NCBI:83333
 
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CA8204-ts 3
 
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''lacY''
 
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*Temperature-dependent (Conditional) growth
 
*Temperature-sensitive growth on lactose medium
 
*Temperature-dependent lactose catabolic process
 
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Growth
 
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exhibited ''lac<sup>+</sup>'' phenotype at 25C, but appeared ''lac<sup>-</sup>'' when grown at 42C
 
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Plating Assay
 
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Figure 1- ONPG hydrolysis ''in vivo''
 
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''Escherichia coli'' K12
 
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NCBI:83333
 
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MS1054
 
|
 
''lacY<sup>-</sup>''
 
|
 
* Absent substrate binding
 
* Absent transporter activity
 
* Abolished lactose catabolic process
 
|
 
Other
 
|
 
no detectable M protein in membrane particulate fractions
 
|
 
Other
 
|
 
Table 2- NEM binding 
 
|-
 
|
 
''Escherichia coli'' K12
 
|
 
NCBI:83333
 
|
 
MS1068
 
|
 
''lacY<sup>-</sup>''
 
|
 
* Absent substrate binding
 
* Absent transporter activity
 
* Abolished lactose catabolic process
 
|
 
Other
 
|
 
no detectable M protein in membrane particulate fractions
 
|
 
Other
 
|
 
Table 2- NEM binding 
 
|-
 
|
 
''Escherichia coli'' K12
 
|
 
NCBI:83333
 
|
 
MS356
 
|
 
''lacY<sup>-</sup>''
 
|
 
* Absent substrate binding
 
* Absent transporter activity
 
* Abolished lactose catabolic process
 
|
 
Other
 
|
 
no detectable M protein in membrane particulate fractions
 
|
 
Other
 
|
 
Table 2- NEM binding 
 
|-
 
|
 
''Escherichia coli'' K12
 
|
 
NCBI:83333
 
|
 
MS1019
 
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''lacY<sup>-</sup>''
 
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absence of substrate binding
 
|
 
Other
 
|
 
no detectable M protein in membrane particulate fractions
 
|
 
Other
 
|
 
Table 2- NEM binding 
 
|-
 
|
 
''Escherichia coli'' K12
 
|
 
NCBI:83333
 
|
 
CA8204
 
|
 
''lacY<sup>-</sup>''
 
|
 
* Absent substrate binding
 
* Absent transporter activity
 
* Abolished lactose catabolic process
 
|
 
Other
 
|
 
no detectable M protein in membrane particulate fractions
 
|
 
Other
 
|
 
Table 2- NEM binding 
 
  
 
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==Notes==
 
==Notes==
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==References==
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{{RefHelp}}
 
<references/>
 
<references/>
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[[Category:Publication]]

Latest revision as of 13:16, 1 September 2013

Citation

No author listed (1967) GENETIC CONTROL OF THE MEMBRANE PROTEIN COMPONENT OF THE LACTOSE TRANSPORT SYSTEM OF Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 57:698-705

Abstract

No abstract in PubMed

Links

PubMed PMC335565

Keywords


Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

</protect>

Notes

References

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