Difference between revisions of "PMID:4882748"

From omp dev
Jump to: navigation, search
(Adding categories)
(change PMID template to TableEdit version)
 
Line 1: Line 1:
 
{{RightTOC}}
 
{{RightTOC}}
−
 
+
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.173.D522384832665f-->
−
<!--box uid=2ccfb3c7bf1208312f02a69e64bfd9e0.173.B4d028452da302-->
 
 
<!--
 
<!--
 
******************************************************************************************
 
******************************************************************************************
Line 8: Line 7:
 
*  
 
*  
 
****************************************************************************************** -->
 
****************************************************************************************** -->
−
{|  id="B4d028452da302"  class=" tableEdit PMID_info_table"   
+
{|  id="D522384832665f"  class=" tableEdit PMID_info_table"   
  
 
|-
 
|-
−
!align=left |Citation
+
!align=left align='left' bgcolor='#CCCCFF' |Citation
 
||
 
||
−
'''Carter, JR, Fox, CF and Kennedy, EP'''  (1968) Interaction of sugars with the membrane protein component of the lactose transport system of Escherichia coli.''Proc. Natl. Acad. Sci. U.S.A.'' '''60''':725-32
+
'''No author listed'''  (1968) Interaction of sugars with the membrane protein component of the lactose transport system of Escherichia coli. ''Proc. Natl. Acad. Sci. U.S.A.'' '''60''':725-32
 
|-
 
|-
−
!align=left |Abstract
+
!align=left align='left' bgcolor='#CCCCFF' |Abstract
 
||
 
||
 
No abstract in PubMed
 
No abstract in PubMed
 
|-
 
|-
−
!align=left |Links
+
!align=left align='left' bgcolor='#CCCCFF' |Links
 
||
 
||
−
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4882748 PubMed]
+
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4882748 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC225106 PMC225106]
  
 
|-
 
|-
−
!align=left |Keywords
+
!align=left align='left' bgcolor='#CCCCFF' |Keywords
 
||
 
||
−
Bacterial Proteins; Binding Sites; Biological Transport, Active; Carbohydrates; Carbon Isotopes; Cell Membrane; Cysteine; Escherichia coli; Ethylmaleimide; Formaldehyde; Galactose; Glycosides; Lactose
+
Bacterial Proteins/metabolism; Binding Sites; Biological Transport, Active; Carbohydrates/pharmacology; Carbon Isotopes; Cell Membrane; Cysteine; Escherichia coli/metabolism; Ethylmaleimide; Formaldehyde/pharmacology; Galactose/pharmacology; Glycosides/pharmacology; Lactose/metabolism
  
 
|- class="tableEdit_footer"  
 
|- class="tableEdit_footer"  
−
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=2ccfb3c7bf1208312f02a69e64bfd9e0.173.B4d028452da302&page=173&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> ||
+
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.173.D522384832665f&page=173&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> ||
 
|}
 
|}
−
<!--box uid=2ccfb3c7bf1208312f02a69e64bfd9e0.173.B4d028452da302-->
+
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.173.D522384832665f-->
−
 
 
 
==Main Points of the Paper ==
 
==Main Points of the Paper ==
−
*The rate of the transport system in intact cells by NEM follows apparent first-order kinetics
+
{{LitSignificance}}
−
**M-TDG reaction: K<sub>D</sub>=5x10<sup>-5</sup> M
 
−
*The rate of the transport system in cell-free fractions by NEM was similar to that in intact cells
 
−
*Melibiose acts similarly to TDG in the NEM labelling, except that the affinity of the sugar for the protein is lower
 
−
*Allolactose had a distinct protective effect
 
  
 
== Materials and Methods Used ==
 
== Materials and Methods Used ==
−
*Parent Strain: ML308 (''i<sup>-</sup>z<sup>+</sup>y<sup>+</sup>a<sup>+</sup>'')
+
{{LitMaterials}}
−
*Membrane vesicle isolation
 
−
*TDG protection
 
−
*NEM labelling of LacY
 
  
 
==Phenotype Annotations==
 
==Phenotype Annotations==
−
<protect><!--box uid=2ccfb3c7bf1208312f02a69e64bfd9e0.173.H4d028452db678-->
+
{{AnnotationTableHelp}}
 +
<protect><!--box uid=d41d8cd98f00b204e9800998ecf8427e.173.Z522384837f227-->
 
<!--
 
<!--
 
******************************************************************************************
 
******************************************************************************************
Line 54: Line 46:
 
*  
 
*  
 
****************************************************************************************** -->
 
****************************************************************************************** -->
−
{|   id="H4d028452db678"  class=" tableEdit PMID_Phenotype_table"   
+
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Z522384837f227"  class=" tableEdit Phenotype_Table_2"   
−
|-
+
|- align='left' bgcolor='#CCCCFF'
−
!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
+
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
−
|-
 
−
|
 
−
''Escherichia coli''
 
−
|
 
−
NCBI:562
 
−
|
 
−
ML308-225
 
−
|
 
−
''lacZ''
 
−
|
 
−
*Abolished beta-galactosidase activity
 
−
*Abolished utilization of carbon source (lactose)
 
−
*Abolished lactose catabolic process
 
−
|
 
−
Metabolic Activity
 
−
|
 
−
*beta-galactosidase negative
 
−
|
 
−
Biochemical Assay
 
−
|
 
−
LacZ assay
 
  
 
|- class="tableEdit_footer"  
 
|- class="tableEdit_footer"  
−
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=2ccfb3c7bf1208312f02a69e64bfd9e0.173.H4d028452db678&page=173&pagename={{FULLPAGENAMEE}}&type=0&template=PMID_Phenotype_table edit table]</span> || || || || || || || ||
+
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.173.Z522384837f227&page=173&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</span> || || || || || || || || ||
 
|}
 
|}
−
<!--box uid=2ccfb3c7bf1208312f02a69e64bfd9e0.173.H4d028452db678--></protect>
+
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.173.Z522384837f227--></protect>
  
 
==Notes==
 
==Notes==
 +
 +
==References==
 +
{{RefHelp}}
 
<references/>
 
<references/>
 +
  
 
[[Category:Publication]]
 
[[Category:Publication]]
−
[[Category:To Be Converted]]
 

Latest revision as of 13:16, 1 September 2013

Citation

No author listed (1968) Interaction of sugars with the membrane protein component of the lactose transport system of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 60:725-32

Abstract

No abstract in PubMed

Links

PubMed PMC225106

Keywords

Bacterial Proteins/metabolism; Binding Sites; Biological Transport, Active; Carbohydrates/pharmacology; Carbon Isotopes; Cell Membrane; Cysteine; Escherichia coli/metabolism; Ethylmaleimide; Formaldehyde/pharmacology; Galactose/pharmacology; Glycosides/pharmacology; Lactose/metabolism

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

Retrieved from "http://bcbp-ftq4xm2.biobio.tamu.edu/omp/dev/index.php?title=PMID:4882748&oldid=10716"