Difference between revisions of "PMID:4869617"

Revision as of 16:21, 6 January 2011

Citation	King, S and Metzger, WI (1968) A new plating medium for the isolation of enteric pathogens. I. hektoen enteric agar.Appl Microbiol 16:577-8
Abstract	A new agar plating medium for the isolation of enteric pathogens is described. This medium contains greater quantities of peptone to offset the inhibitory effects of bile salts. Additional carbohydrates and larger quantities of those previously used have also been incorporated into the medium to differentiate pathogens from some of the slow lactose fermenters. The use of an indicator system never used before in a differential plating medium opens new possibilities for improvement over traditional and newer selective media. Known strains from the various genera of Enterobacteriaceae have been tested for colonial differentiation as well as for possible inhibitory effects. The results show the medium to be highly selective for the shigellae as well as for other enteric pathogens.
Links	PubMed
Keywords	Agar; Bile Acids and Salts; Enterobacter; Enterobacteriaceae; Escherichia coli; Indicators and Reagents; Proteus; Salmonella; Shigella
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Describes the development of Hektoen Agar (HE)
- selective for Shigella as well as other pathogens while inhibiting normal intestinal flora
- contains several indicators which allow for colonies to be specifically identified, increasing the confidence of the identification

Post-Boiling Additives
- Sodium thiosulfate
- Ferric ammonium citrate
- Sodium deoxycholate
- H₂S indicator

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Species	Taxon ID	Strain	Gene (if known)	OMP	Phenotype	Details	Evidence	Notes
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 == Materials and Methods Used ==
-{{LitMaterials}}
+*Medium contains:
+**Lactose
+**Sucrose
+**Salicin
+**Bile salts
+**Sodium chloride
+**Proteose peptone
+**Beef extract
+**Agar
+**Bromothymol blue
+**Andrades' indicator
+*Post-Boiling Additives
+**Sodium thiosulfate
+**Ferric ammonium citrate
+**Sodium deoxycholate
+**H<sub>2</sub>S indicator
+*Strains
+**''Escherichia coli''
+**''Citrobacter''
+**''Proteus mirabilis''
+**''Paracolobactrum arizonae''
+**''Salmonella''
+**''Shigella''
+**''Pseudomonas''
+**''Providencia''
 ==Phenotype Annotations==