Difference between revisions of "PMID:12997228"
(Table edited by Azweifel via TableEdit) |
(Table edited by Azweifel via TableEdit) |
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| − | + | *Abolished utilization of carbon source (lactose) | |
| + | *Growth phenotype on EMB (grows on EMB, but has negative result on indicator for lactose) | ||
| + | *Abolished lactose catabolic process | ||
| | | | ||
Growth | Growth | ||
| | | | ||
| − | cannot utilize lactose | + | *cannot utilize lactose |
| + | *deficient in lactose metabolism | ||
| | | | ||
Plating Assay | Plating Assay | ||
| | | | ||
| − | plated on eosin-methylene blue agar | + | plated on eosin-methylene blue agar |
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| Line 79: | Line 82: | ||
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| − | + | *Utilization of carbon source (melibiose) | |
| + | *Growth on EMB (positive result on indicator) | ||
| + | *Melibiose catabolic process | ||
| + | *Increased rate of? | ||
| | | | ||
Growth | Growth | ||
| | | | ||
The authors compared the ability of different strains to ferment different sugars | The authors compared the ability of different strains to ferment different sugars | ||
| + | *ferment melibiose rapidly compared to control | ||
| | | | ||
Plating Assay | Plating Assay | ||
| | | | ||
| − | plated on eosin-methylene blue agar with 1% | + | plated on eosin-methylene blue agar with 1% melibiose |
|- | |- | ||
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| Line 98: | Line 105: | ||
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| − | + | *Utilization of carbon source (xylose) | |
| + | *Xylose catabolic process | ||
| + | *Reduced rate of? | ||
| + | *Growth on EMB (positive result for indicator) | ||
| | | | ||
Growth | Growth | ||
| | | | ||
The authors compared the ability of different strains to ferment different sugars | The authors compared the ability of different strains to ferment different sugars | ||
| + | *ferment xylose slowly compared to control | ||
| | | | ||
Plating Assay | Plating Assay | ||
| | | | ||
| − | plated on eosin-methylene blue agar with 1% | + | plated on eosin-methylene blue agar with 1% xylose |
|- | |- | ||
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| Line 117: | Line 128: | ||
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| − | + | *Abolished carbon source utilization (lactose) | |
| + | *Abolished lactose catabolic process | ||
| + | *Growth on EMB (negative result on indicator) | ||
| + | |||
| | | | ||
Growth | Growth | ||
| | | | ||
| − | cannot utilize lactose | + | *cannot utilize lactose |
| + | *deficient in lactose metabolism | ||
| | | | ||
Plating Assay | Plating Assay | ||
| | | | ||
| − | plated on eosin-methylene blue agar | + | plated on eosin-methylene blue agar |
|- class="tableEdit_footer" | |- class="tableEdit_footer" | ||
Revision as of 15:10, 26 January 2011
| Citation |
LESTER, G (1952) The beta-galactosidase of lactose mutants of Escherichia coli, K-12.Arch. Biochem. Biophys. 40:390-401 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Escherichia coli; Glycoside Hydrolases |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Escherichia coli |
NCBI:562 |
Y-53 |
|
Growth |
|
Plating Assay |
plated on eosin-methylene blue agar | |
|
Escherichia coli |
NCBI:562 |
Y-53 |
|
Growth |
The authors compared the ability of different strains to ferment different sugars
|
Plating Assay |
plated on eosin-methylene blue agar with 1% melibiose | |
|
Escherichia coli |
NCBI:562 |
Y-53 |
|
Growth |
The authors compared the ability of different strains to ferment different sugars
|
Plating Assay |
plated on eosin-methylene blue agar with 1% xylose | |
|
Escherichia coli |
NCBI:562 |
W-45 |
|
Growth |
|
Plating Assay |
plated on eosin-methylene blue agar | |
| edit table |
</protect>
