Difference between revisions of "PMID:7287890"
(Fill PMID: Page!) |
(Fill PMID: Page!) |
||
| (144 intermediate revisions by 33 users not shown) | |||
| Line 1: | Line 1: | ||
{{RightTOC}} | {{RightTOC}} | ||
| − | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.3513. | + | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.3513.S5b59a248b7a09--> |
<!-- | <!-- | ||
****************************************************************************************** | ****************************************************************************************** | ||
| Line 8: | Line 8: | ||
* | * | ||
****************************************************************************************** --> | ****************************************************************************************** --> | ||
| − | {| id=" | + | {| id="S5b59a248b7a09" class=" tableEdit PMID_info_table" |
|- | |- | ||
| Line 29: | Line 29: | ||
|- class="tableEdit_footer" | |- class="tableEdit_footer" | ||
| − | |< | + | |<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3513.S5b59a248b7a09&page=3513&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</div> || |
|} | |} | ||
| − | + | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.3513.S5b59a248b7a09--> | |
| − | |||
| − | |||
| − | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.3513. | ||
==Main Points of the Paper == | ==Main Points of the Paper == | ||
| Line 44: | Line 41: | ||
==Phenotype Annotations== | ==Phenotype Annotations== | ||
{{AnnotationTableHelp}} | {{AnnotationTableHelp}} | ||
| − | <protect><!--box uid=d41d8cd98f00b204e9800998ecf8427e.3513. | + | <protect><!--box uid=d41d8cd98f00b204e9800998ecf8427e.3513.J5b59a248e96ca--> |
<!-- | <!-- | ||
****************************************************************************************** | ****************************************************************************************** | ||
| Line 51: | Line 48: | ||
* | * | ||
****************************************************************************************** --> | ****************************************************************************************** --> | ||
| − | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id=" | + | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="J5b59a248e96ca" class=" tableEdit Phenotype_Table_2" |
|- align='left' bgcolor='#CCCCFF' | |- align='left' bgcolor='#CCCCFF' | ||
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
|- class="tableEdit_footer" | |- class="tableEdit_footer" | ||
| − | |< | + | |<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3513.J5b59a248e96ca&page=3513&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</div> || || || || || || || || || |
|} | |} | ||
| − | + | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.3513.J5b59a248e96ca--></protect> | |
| − | |||
| − | |||
| − | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.3513. | ||
==Notes== | ==Notes== | ||
Latest revision as of 05:28, 26 July 2018
| Citation |
Wallis, C, Melnick, JL and Longoria, CJ (1981) Colorimetric method for rapid determination of bacteriuria. J. Clin. Microbiol. 14:342-6 |
|---|---|
| Abstract |
An inexpensive, rapid, and simple colorimetric test for detection of bacteriuria is described. This test does not require bacterial growth and has the marked advantage of being able to quantify bacteria, even when the organisms are present in the urine of bacteriuric patients who are being treated with antibiotics. The test is carried out with 1 ml of urine, which is processed through a 10-mm-diameter filter than entraps the bacteria on its surface. Safranine dye is passed through the filter to stain the bacteria and the filter fibers. A decolorizer, which removes the dye from the filter fibers but not from the bacteria, is then passed through the filter. If there are greater than or equal to 10(5) colony-forming units of bacteria per ml in the sample, the 10-mm filter disk manifests a pink to red color. If there are less than 10(5)colony-forming units of bacteria per ml, the filter disk remains white or becomes slightly yellow. The entire procedure has been adapted to a semiautomated instrument and the time required per test is less than 1 min. The results obtained on the test card are a permanent record to be filled with the patient's chart. The bacteria can be quickly classified as gram positive or gram negative by selective staining of a second milliliter of urine on the filter. Of 441 urine specimens tested, 430 (98%) were correctly classified as containing more or less than 10(5) colony-forming units per ml. A total of 62 urine specimens were positive by bacterial plating (greater than or equal to 10(5) colony-forming units per ml), and 59 were positive by the colorimetric test. Eight false-positives (colorimetric test positive, plate counts less than 10(5)) were encountered in patients (bacteriuric) being prescribed antibiotics. Removal of the antibiotics from these urine specimens, with subsequent replating of the samples, indicated the presence of greater than or equal to 10(5) colony-forming units of bacteria per ml in three representative cases tested, indicating that the results of the colorimetric tests were not false-positives but that the plate counts were low because of the inhibition of bacterial growth by the residual antibiotics present in the urine. |
| Links | |
| Keywords |
Bacteria/isolation & purification; Bacteriuria/diagnosis; Colorimetry/methods; Coloring Agents; Gentian Violet/diagnostic use; Humans; Indicators and Reagents; Phenazines/diagnostic use; Species Specificity; Urine/microbiology |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
</protect>
Notes
References
See Help:References for how to manage references in omp dev.
