Difference between revisions of "PMID:21541325"

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(Table edited by Siegele via TableEdit)
(Table edited by Siegele via TableEdit)
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
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*Genotype of Reference Strain: tolC
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*Genotype of Reference Strain: ''tolC<sup>+</sup>''
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*Genotype of Experimental Strain : ''tolC732::Kan''
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*Genotype of Experimental Strain : ''tolC732(del)::kan''
 
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*Reference Condition:
 
*Reference Condition:
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TolC was required for EXTREME-acid survival (pH 2) of strain W3110 grown aerobically to stationary phase. A tolC deletion decreased extreme-acid survival (acid resistance) of aerated pH 7.0-grown cells by 105-fold and of pH 5.5-grown cells by 10-fold. TolC was required for expression of glutamate decarboxylase (GadA, GadB), a key component of glutamate-dependent acid resistance (Gad). TolC was also required for maximal exponential growth of E. coli K-12 W3110, in LBK medium buffered at pH 4.5-6.0, but not at pH 6.5-8.5. The TolC growth requirement in moderate acid was independent of Gad.
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Figure 1:  decreased survival at pH 2.
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JLS1015 = W3110 tolC732(del)::kan (Keio JW5503)
 
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Revision as of 17:13, 15 January 2015

Citation

Deininger, KN, Horikawa, A, Kitko, RD, Tatsumi, R, Rosner, JL, Wachi, M and Slonczewski, JL (2011) A requirement of TolC and MDR efflux pumps for acid adaptation and GadAB induction in Escherichia coli.PLoS ONE 6:e18960

Abstract

The TolC outer membrane channel is a key component of several multidrug resistance (MDR) efflux pumps driven by H(+) transport in Escherichia coli. While tolC expression is under the regulation of the EvgA-Gad acid resistance regulon, the role of TolC in growth at low pH and extreme-acid survival is unknown.

Links

PubMed Online version:10.1371/journal.pone.0018960

Keywords

Acids; Adaptation, Physiological; Bacterial Outer Membrane Proteins; Drug Resistance, Multiple; Enzyme Induction; Escherichia coli K12; Escherichia coli Proteins; Glutamate Decarboxylase; Glutamates; Hydrogen-Ion Concentration; Membrane Proteins; Membrane Transport Proteins; Microbial Viability; Mutation

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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JLS1015
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: tolC+
  • Genotype of Experimental Strain : tolC732(del)::kan
  • Reference Condition:

OMP:0005077

decreased resistance to acidic pH stres

Figure 1: decreased survival at pH 2.

JLS1015 = W3110 tolC732(del)::kan (Keio JW5503)


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</protect>

Notes

References

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