PMID:21541325

Citation	Deininger, KN, Horikawa, A, Kitko, RD, Tatsumi, R, Rosner, JL, Wachi, M and Slonczewski, JL (2011) A requirement of TolC and MDR efflux pumps for acid adaptation and GadAB induction in Escherichia coli.PLoS ONE 6:e18960
Abstract	The TolC outer membrane channel is a key component of several multidrug resistance (MDR) efflux pumps driven by H(+) transport in Escherichia coli. While tolC expression is under the regulation of the EvgA-Gad acid resistance regulon, the role of TolC in growth at low pH and extreme-acid survival is unknown.
Links	PubMed Online version:10.1371/journal.pone.0018960
Keywords	Acids; Adaptation, Physiological; Bacterial Outer Membrane Proteins; Drug Resistance, Multiple; Enzyme Induction; Escherichia coli K12; Escherichia coli Proteins; Glutamate Decarboxylase; Glutamates; Hydrogen-Ion Concentration; Membrane Proteins; Membrane Transport Proteins; Microbial Viability; Mutation
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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JLS1024 NCBI Taxon ID: 83333	Genotype of Reference Strain: mdtB+ Genotype of Experimental Strain : mdtB774(del)::kan	Reference Condition:	OMP:0005077	decreased resistance to acidic pH stress			Figure 1: decreased survival at pH 2. JLS1024 = W3110 mdtB774(del)::kan (Keio JW2060)
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JLS1015 NCBI Taxon ID: 83333	Genotype of Reference Strain: tolC+ Genotype of Experimental Strain : tolC732(del)::kan	Reference Condition:	OMP:0005077	decreased resistance to acidic pH stress			Figure 1: decreased survival at pH 2. JLS1015 = W3110 tolC732(del)::kan (Keio JW5503)
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JLS1027 NCBI Taxon ID: 83333	Genotype of Reference Strain: emrB+ Genotype of Experimental Strain : emrB767(del)::kan	Reference Condition:	OMP:0005077	decreased resistance to acidic pH stress			Figure 1: decreased survival at pH 2. JLS1027 = W3110 emrB767(del)::kan (Keio JW2661)
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JLS1015 NCBI Taxon ID: 83333	Genotype of Reference Strain: tolC+ Genotype of Experimental Strain : tolC732(del)::kan	Reference Condition:	OMP:0007008	decreased growth at acidic pH			Figure 4: no growth at pH 4.5 JLS1015 = W3110 tolC732(del)::kan (Keio JW5503)
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: MG1655 NCBI Taxon ID: 83333	Genotype of Reference Strain: tolC+ Genotype of Experimental Strain : tolC::Tn10	Reference Condition:	OMP:0007118	decreased transcription			Figure 2B: undetectable level of gadA mRNA at pH 5.5
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: MG1655 NCBI Taxon ID: 83333	Genotype of Reference Strain: tolC+ Genotype of Experimental Strain : tolC::Tn10	Reference Condition:	OMP:0000268	decreased gene expression			Figures 2A, 2C: Decreased glutamate decarboxylase activity and decreased GadA/B protein levels at pH 5.5.
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