Difference between revisions of "PMID:21541325"

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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
 
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: JLS1024
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
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*Genotype of Reference Strain: ''mdtB<sup>+</sup>''
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*Genotype of Experimental Strain : ''mdtB774(del)::kan''
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*Reference Condition:
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OMP:0005077
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decreased resistance to acidic pH stress
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 +
 +
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Figure 1:  decreased survival at pH 2.
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JLS1024 = W3110 ''mdtB774(del)::kan'' (Keio JW2060)
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: JLS1015
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
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*Genotype of Reference Strain: ''tolC<sup>+</sup>''
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*Genotype of Experimental Strain : ''tolC732(del)::kan''
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*Reference Condition:
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OMP:0005077
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|
 +
decreased resistance to acidic pH stress
 +
|
 +
 +
|
 +
 +
|
 +
Figure 1:  decreased survival at pH 2.
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JLS1015 = W3110 tolC732(del)::kan (Keio JW5503)
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|
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 +
|-
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: JLS1027
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
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*Genotype of Reference Strain: ''emrB<sup>+</sup>''
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*Genotype of Experimental Strain : ''emrB767(del)::kan''
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*Reference Condition:
 +
|
 +
OMP:0005077
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|
 +
decreased resistance to acidic pH stress
 +
|
 +
 +
|
 +
 +
|
 +
Figure 1:  decreased survival at pH 2.
 +
 +
JLS1027 = W3110 ''emrB767(del)::kan'' (Keio JW2661)
 +
|
 +
 +
|-
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a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: JLS1015
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''tolC<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''tolC732(del)::kan''
 +
|
 +
*Reference Condition:
 +
|
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OMP:0007008
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|
 +
decreased growth at acidic pH
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|
 +
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|
 +
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Figure 4:  no growth at pH 4.5
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JLS1015 = W3110 tolC732(del)::kan (Keio JW5503)
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|
 +
 +
|-
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|
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a mutation or genetic difference within a strain
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|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
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*Substrain: MG1655
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
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|
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*Genotype of Reference Strain: ''tolC<sup>+</sup>''
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*Genotype of Experimental Strain : ''tolC::Tn10''
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*Reference Condition:
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|
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OMP:0007118
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decreased transcription
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ECO:0000106
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Northern assay evidence
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Figure 2B: undetectable level of ''gadA'' mRNA at pH 5.5
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|-
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a mutation or genetic difference within a strain
 +
|
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*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: MG1655
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''tolC<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''tolC::Tn10''
 +
|
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*Reference Condition:
 +
|
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OMP:0000268
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|
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decreased gene expression
 +
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Figures 2A, 2C: Decreased glutamate decarboxylase activity and decreased GadA/B protein levels at pH 5.5.
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Latest revision as of 17:33, 15 January 2015

Citation

Deininger, KN, Horikawa, A, Kitko, RD, Tatsumi, R, Rosner, JL, Wachi, M and Slonczewski, JL (2011) A requirement of TolC and MDR efflux pumps for acid adaptation and GadAB induction in Escherichia coli.PLoS ONE 6:e18960

Abstract

The TolC outer membrane channel is a key component of several multidrug resistance (MDR) efflux pumps driven by H(+) transport in Escherichia coli. While tolC expression is under the regulation of the EvgA-Gad acid resistance regulon, the role of TolC in growth at low pH and extreme-acid survival is unknown.

Links

PubMed Online version:10.1371/journal.pone.0018960

Keywords

Acids; Adaptation, Physiological; Bacterial Outer Membrane Proteins; Drug Resistance, Multiple; Enzyme Induction; Escherichia coli K12; Escherichia coli Proteins; Glutamate Decarboxylase; Glutamates; Hydrogen-Ion Concentration; Membrane Proteins; Membrane Transport Proteins; Microbial Viability; Mutation

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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JLS1024
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: mdtB+
  • Genotype of Experimental Strain : mdtB774(del)::kan
  • Reference Condition:

OMP:0005077

decreased resistance to acidic pH stress

Figure 1: decreased survival at pH 2.

JLS1024 = W3110 mdtB774(del)::kan (Keio JW2060)

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JLS1015
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: tolC+
  • Genotype of Experimental Strain : tolC732(del)::kan
  • Reference Condition:

OMP:0005077

decreased resistance to acidic pH stress

Figure 1: decreased survival at pH 2.

JLS1015 = W3110 tolC732(del)::kan (Keio JW5503)

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JLS1027
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: emrB+
  • Genotype of Experimental Strain : emrB767(del)::kan
  • Reference Condition:

OMP:0005077

decreased resistance to acidic pH stress

Figure 1: decreased survival at pH 2.

JLS1027 = W3110 emrB767(del)::kan (Keio JW2661)

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JLS1015
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: tolC+
  • Genotype of Experimental Strain : tolC732(del)::kan
  • Reference Condition:

OMP:0007008

decreased growth at acidic pH

Figure 4: no growth at pH 4.5

JLS1015 = W3110 tolC732(del)::kan (Keio JW5503)

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: MG1655
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: tolC+
  • Genotype of Experimental Strain : tolC::Tn10
  • Reference Condition:

OMP:0007118

decreased transcription

ECO:0000106

Northern assay evidence

Figure 2B: undetectable level of gadA mRNA at pH 5.5

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: MG1655
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: tolC+
  • Genotype of Experimental Strain : tolC::Tn10
  • Reference Condition:

OMP:0000268

decreased gene expression

Figures 2A, 2C: Decreased glutamate decarboxylase activity and decreased GadA/B protein levels at pH 5.5.


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Notes

References

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