Difference between revisions of "PMID:21453489"

From omp dev
Jump to: navigation, search
(Table edited by Wameza via TableEdit)
(Table edited by Siegele via TableEdit)
 
(2 intermediate revisions by one other user not shown)
Line 48: Line 48:
 
*  
 
*  
 
****************************************************************************************** -->
 
****************************************************************************************** -->
−
{|   id="R4ef7feceb0777"  class=" tableEdit Phenotype_Table_2"   
+
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="R4ef7feceb0777"  class=" tableEdit Phenotype_Table_2"   
−
|-
+
|- align='left' bgcolor='#CCCCFF'
 
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
 
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
 
|-  
 
|-  
Line 57: Line 57:
 
*Taxon: Escherichia coli
 
*Taxon: Escherichia coli
 
*Strain: K-12
 
*Strain: K-12
−
*Substrain: TP1204
+
*Substrain: MG1655
 
*NCBI Taxon ID: 8333
 
*NCBI Taxon ID: 8333
 
|
 
|
−
*Genotype of Reference Strain: ''greA''
+
*Genotype of Reference Strain: ''greA<sup>+</sup>''
−
*Genotype of Experimental Strain : ''greA -D41A''
+
*Genotype of Experimental Strain : ''greA-D41A''
 
|
 
|
 
*Reference Condition:
 
*Reference Condition:
 +
|
 +
OMP:0005050
 +
|
 +
decreased frequency of homologous recombination
 +
|
 +
 +
|
 +
 +
|
 +
Figure 1 and Figure 2:  decreased lambda Red-mediated recombination
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: MG1655
 +
*NCBI Taxon ID: 8333
 +
|
 +
*Genotype of Reference Strain: ''greA<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''greA-D41N''
 +
|
 +
*Reference Condition:
 +
|
 +
OMP:0005050
 +
|
 +
decreased frequency of homologous recombination
 +
|
 +
 +
|
 +
 +
|
 +
Figure 1 and Figure 2:  decreased lambda Red-mediated recombination
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: MG1655
 +
*NCBI Taxon ID: 8333
 +
|
 +
*Genotype of Reference Strain: ''greA<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''greA-E44K''
 +
|
 +
*Reference Condition:
 +
|
 +
OMP:0005050
 +
|
 +
decreased frequency of homologous recombination
 
|
 
|
  
 
|
 
|
  
 +
|
 +
Figure 1 and Figure 2:  decreased lambda Red-mediated recombination
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: MG1655
 +
*NCBI Taxon ID: 8333
 +
|
 +
*Genotype of Reference Strain: ''greA<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''greA(del)''
 +
|
 +
*Reference Condition:
 +
|
 +
OMP:0005050
 +
|
 +
decreased frequency of homologous recombination
 
|
 
|
  
Line 73: Line 148:
  
 
|
 
|
−
The substitution of one Amino Acid for another caused a reduction in lambda red-mediated recombination
+
Figure 1 and Figure 2:  decreased lambda Red-mediated recombination
 
|
 
|
 
   
 
   
Line 80: Line 155:
 
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.2826.R4ef7feceb0777&page=2826&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</span> || || || || || || || || ||
 
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.2826.R4ef7feceb0777&page=2826&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</span> || || || || || || || || ||
 
|}
 
|}
 +
<noinclude>
 +
 +
</noinclude>
 
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.2826.R4ef7feceb0777--></protect>
 
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.2826.R4ef7feceb0777--></protect>
  

Latest revision as of 18:11, 15 January 2015

Citation

Poteete, AR (2011) Recombination phenotypes of Escherichia coli greA mutants.BMC Mol. Biol. 12:12

Abstract

The elongation factor GreA binds to RNA polymerase and modulates transcriptional pausing. Some recent research suggests that the primary role of GreA may not be to regulate gene expression, but rather, to promote the progression of replication forks which collide with RNA polymerase, and which might otherwise collapse. Replication fork collapse is known to generate dsDNA breaks, which can be recombinogenic. It follows that GreA malfunction could have consequences affecting homologous recombination.

Links

PubMed Online version:10.1186/1471-2199-12-12

Keywords

Escherichia coli; Escherichia coli Proteins; Mutation; Phenotype; Rec A Recombinases; Recombination, Genetic; Transcription Factors

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: MG1655
  • NCBI Taxon ID: 8333
  • Genotype of Reference Strain: greA+
  • Genotype of Experimental Strain : greA-D41A
  • Reference Condition:

OMP:0005050

decreased frequency of homologous recombination

Figure 1 and Figure 2: decreased lambda Red-mediated recombination

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: MG1655
  • NCBI Taxon ID: 8333
  • Genotype of Reference Strain: greA+
  • Genotype of Experimental Strain : greA-D41N
  • Reference Condition:

OMP:0005050

decreased frequency of homologous recombination

Figure 1 and Figure 2: decreased lambda Red-mediated recombination

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: MG1655
  • NCBI Taxon ID: 8333
  • Genotype of Reference Strain: greA+
  • Genotype of Experimental Strain : greA-E44K
  • Reference Condition:

OMP:0005050

decreased frequency of homologous recombination

Figure 1 and Figure 2: decreased lambda Red-mediated recombination

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: MG1655
  • NCBI Taxon ID: 8333
  • Genotype of Reference Strain: greA+
  • Genotype of Experimental Strain : greA(del)
  • Reference Condition:

OMP:0005050

decreased frequency of homologous recombination

Figure 1 and Figure 2: decreased lambda Red-mediated recombination


edit table


</protect>

Notes

References

See Help:References for how to manage references in omp dev.

Retrieved from "http://bcbp-ftq4xm2.biobio.tamu.edu/omp/dev/index.php?title=PMID:21453489&oldid=13766"