Difference between revisions of "PMID:4599365"
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temperature sensitive defect in initiation of DNA replication | temperature sensitive defect in initiation of DNA replication | ||
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| − | + | Metabolic Activity | |
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| − | + | thymidine incorporation | |
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| − | + | Biochemical Assay | |
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| − | + | Figure 4A | |
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| + | Hfr-120/6 | ||
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| + | temperature sensitive defect in initiation of DNA replication | ||
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| + | ''Escherichia coli'' | ||
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| + | NS476 | ||
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| + | dnaA366(Am) | ||
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| + | temperature sensitive defect in initiation of DNA replication | ||
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| + | Metabolic Activity | ||
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| + | thymidine incorporation | ||
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| + | Biochemical Assay | ||
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| + | Figure 4B | ||
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| + | ''Escherichia coli'' | ||
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| + | NS477 | ||
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| + | dnaA91(Am) | ||
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| + | temperature sensitive defect in initiation of DNA replication | ||
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| + | Metabolic Activity | ||
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| + | thymidine incorporation | ||
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| + | Biochemical Assay | ||
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| + | Figure 4C | ||
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Revision as of 14:15, 9 March 2011
| Citation |
Goebel, W (1974) Integrative suppression of temperature-sensitive mutants iwth a lesion in the initiation of DNA replication. Replication of autonomous plasmids in the suppressed state.Eur. J. Biochem. 43:125-30 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Centrifugation, Density Gradient; Chromosome Mapping; DNA Replication; Drug Resistance, Microbial; Escherichia coli; Extrachromosomal Inheritance; Gene Frequency; Mutation; Species Specificity; Streptomycin; Suppression, Genetic; Temperature; Thymine; Tritium |
| edit table |
Main Points of the Paper
- amber mutations in dnaA were isolated
Materials and Methods Used
- Integrative suppression
- EtBr sensitivity assay
- Monitor growth rate
- Thymidine incorporation to measure DNA synthesis rate
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
CRT-46 |
streptomycin resistant |
Resistance to |
Plating Assay |
|||||
|
CRT-46 |
abolished lactose utilization |
Metabolic Activity |
Plating Assay |
|||||
|
CRT-46 |
threonine auxotroph |
Growth |
Plating Assay |
|||||
|
CRT-46 |
abolished lactose utilization |
Metabolic Activity |
Plating Assay |
|||||
|
CRT-46 |
leucine auxotroph |
Growth |
Plating Assay |
|||||
|
CRT-46 |
isoleucine auxotroph |
Growth |
Plating Assay |
|||||
|
CRT-46 |
thiamine auxotroph |
Growth |
Plating Assay |
|||||
|
CRT-46 |
thymine auxotroph |
Growth |
Plating Assay |
|||||
|
Escherichia coli |
NS475 |
dnaA311(Am) |
temperature sensitive defect in initiation of DNA replication |
Metabolic Activity |
thymidine incorporation |
Biochemical Assay |
Figure 4A | |
|
Escherichia coli |
Hfr-120/6 |
thiamine auxotroph |
Growth |
Plating Assay |
||||
|
Escherichia coli |
Hfr-120/6 |
thymine auxotroph |
Growth |
Plating Assay |
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|
Escherichia coli |
PC2 |
thymine auxotroph |
Growth |
Plating Assay |
||||
|
Escherichia coli |
PC2 |
leucine auxotroph |
Growth |
Plating Assay |
||||
|
Escherichia coli |
PC2 |
streptomyin resistant |
||||||
|
Escherichia coli |
NCBI: |
Hfr-120/6 |
temperature sensitive defect in initiation of DNA replication |
|||||
|
Escherichia coli |
NS476 |
dnaA366(Am) |
temperature sensitive defect in initiation of DNA replication |
Metabolic Activity |
thymidine incorporation |
Biochemical Assay |
Figure 4B | |
|
Escherichia coli |
NS477 |
dnaA91(Am) |
temperature sensitive defect in initiation of DNA replication |
Metabolic Activity |
thymidine incorporation |
Biochemical Assay |
Figure 4C | |
| edit table |
</protect>
Notes
References
See Help:References for how to manage references in omp dev.
