PMID:17824928

Citation	Danilova, O, Reyes-Lamothe, R, Pinskaya, M, Sherratt, D and Possoz, C (2007) MukB colocalizes with the oriC region and is required for organization of the two Escherichia coli chromosome arms into separate cell halves.Mol. Microbiol. 65:1485-92
Abstract	The circular Escherichia coli chromosome is organized by bidirectional replication into two equal left and right arms (replichores). Each arm occupies a separate cell half, with the origin of replication (oriC) at mid-cell. E. coli MukBEF belongs to the ubiquitous family of SMC protein complexes that play key roles in chromosome organization and processing. In mukBEF mutants, viability is restricted to low temperature with production of anucleate cells, reflecting chromosome segregation defects. We show that in mukB mutant cells, the two chromosome arms do not separate into distinct cell halves, but extend from pole to pole with the oriC region located at the old pole. Mutations in topA, encoding topoisomerase I, do not suppress the aberrant positioning of chromosomal loci in mukB cells, despite suppressing the temperature-sensitivity and production of anucleate cells. Furthermore, we show that MukB and the oriC region generally colocalize throughout the cell cycle, even when oriC localization is aberrant. We propose that MukBEF initiates the normal bidirectional organization of the chromosome from the oriC region.
Links	PubMed Online version:10.1111/j.1365-2958.2007.05881.x
Keywords	Cell Polarity; Chromosomal Proteins, Non-Histone; Chromosome Segregation; Chromosomes, Bacterial; Escherichia coli; Escherichia coli Proteins; Models, Genetic; Mutation; Protein Transport; Replication Origin
edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Species	Taxon ID	Strain	Gene (if known)	OMP	Phenotype	Details	Evidence	Notes
Escherichia coli			mukB	aberrant chromosome segregation			Microscopy
edit table

</protect>

References

See Help:References for how to manage references in omp dev.