Difference between revisions of "Southern Blot Analysis"
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== Materials Needed == | == Materials Needed == | ||
| + | -DNA sample | ||
| + | |||
| + | -0.25 MHCl | ||
| + | |||
| + | -Denaturation Solution (1.5 M NaCl/0.5 M NaOH) | ||
| + | |||
| + | -Neutralization Solution (1.5 M NaCl/0.5 M Tris-Cl) | ||
| + | |||
| + | -20X and 2X SSC | ||
| + | |||
| + | -Sponge (larger than gel to be blotted) | ||
| + | |||
| + | -Whatman 3 MM filter paper sheets | ||
| + | |||
| + | -Nylon membrane | ||
| + | |||
| + | -UV-transparent plastic wrap | ||
| + | |||
| + | -UV transilluminator | ||
== Protocol == | == Protocol == | ||
Revision as of 14:13, 13 July 2015
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Contents
Purpose
This protocol is used to immobilize DNA fragments so the DNA can be used for Hybridization Analysis.
Materials Needed
-DNA sample
-0.25 MHCl
-Denaturation Solution (1.5 M NaCl/0.5 M NaOH)
-Neutralization Solution (1.5 M NaCl/0.5 M Tris-Cl)
-20X and 2X SSC
-Sponge (larger than gel to be blotted)
-Whatman 3 MM filter paper sheets
-Nylon membrane
-UV-transparent plastic wrap
-UV transilluminator
Protocol
Notes
Papers where this or a similar method has been used
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Related Ontology Terms
References
See Help:References for how to manage references in omp dev.
