Difference between revisions of "PMID:810485"

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'''Brehm, SP, Le Hegarat, F and Hoch, JA'''  (1975) Deoxyribonucleic acid-binding proteins in vegetative Bacillus subtilis: alterations caused by stage O sporulation mutations. ''J. Bacteriol.'' '''124''':977-84
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!align=left align='left' bgcolor='#CCCCFF' |Abstract
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Deoxyribonucleic acid (DNA)-binding proteins have been compared between wild-type Bacillus subtilis and five sporulation mutants blocked at different stage O loci. Extracts from exponentially growing cells have been fractionated for proteins binding to single-stranded calf thymus DNA-cellulose and double-stranded B. subtilis DNA-cellulose. In nutrient broth, stage O mutations cause an accumulation of proteins with affinity for double-stranded DNA. Suppression of the mutation with extragenic suppressors relieves the accumulation. In minimal glucose medium, the stage O mutations also cause accumulation of proteins with affinity for double-stranded DNA, but the species accumulated are different from those of nutrient broth-grown cells. In neither case did stage O mutations affect proteins with affinity for single-stranded DNA. The results suggest that the products of stage O loci are functional and operative during vegetative growth.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=810485 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC235988 PMC235988]
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!align=left align='left' bgcolor='#CCCCFF' |Keywords
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Bacillus subtilis/growth & development; Bacillus subtilis/metabolism; Bacterial Proteins/metabolism; Culture Media; DNA/metabolism; DNA, Bacterial/metabolism; DNA, Single-Stranded/metabolism; Glucose/metabolism; Molecular Weight; Mutation; Protein Binding; Spores, Bacterial/growth & development; Suppression, Genetic
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Latest revision as of 12:00, 16 August 2015

Citation

Brehm, SP, Le Hegarat, F and Hoch, JA (1975) Deoxyribonucleic acid-binding proteins in vegetative Bacillus subtilis: alterations caused by stage O sporulation mutations. J. Bacteriol. 124:977-84

Abstract

Deoxyribonucleic acid (DNA)-binding proteins have been compared between wild-type Bacillus subtilis and five sporulation mutants blocked at different stage O loci. Extracts from exponentially growing cells have been fractionated for proteins binding to single-stranded calf thymus DNA-cellulose and double-stranded B. subtilis DNA-cellulose. In nutrient broth, stage O mutations cause an accumulation of proteins with affinity for double-stranded DNA. Suppression of the mutation with extragenic suppressors relieves the accumulation. In minimal glucose medium, the stage O mutations also cause accumulation of proteins with affinity for double-stranded DNA, but the species accumulated are different from those of nutrient broth-grown cells. In neither case did stage O mutations affect proteins with affinity for single-stranded DNA. The results suggest that the products of stage O loci are functional and operative during vegetative growth.

Links

PubMed PMC235988

Keywords

Bacillus subtilis/growth & development; Bacillus subtilis/metabolism; Bacterial Proteins/metabolism; Culture Media; DNA/metabolism; DNA, Bacterial/metabolism; DNA, Single-Stranded/metabolism; Glucose/metabolism; Molecular Weight; Mutation; Protein Binding; Spores, Bacterial/growth & development; Suppression, Genetic


Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

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<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status


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Notes

References

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