Difference between revisions of "PMID:11435407"
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| + | {| id="C4dbaede7c2ece" class=" tableEdit PMID_info_table" | ||
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| + | !align=left |Citation | ||
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| + | '''Bochner, BR, Gadzinski, P and Panomitros, E''' (2001) Phenotype microarrays for high-throughput phenotypic testing and assay of gene function.''Genome Res.'' '''11''':1246-55 | ||
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| + | !align=left |Abstract | ||
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| + | The bacterium Escherichia coli is used as a model cellular system to test and validate a new technology called Phenotype MicroArrays (PMs). PM technology is a high-throughput technology for simultaneous testing of a large number of cellular phenotypes. It consists of preconfigured well arrays in which each well tests a different cellular phenotype and an automated instrument that continuously monitors and records the response of the cells in all wells of the arrays. For example, nearly 700 phenotypes of E. coli can be assayed by merely pipetting a cell suspension into seven microplate arrays. PMs can be used to directly assay the effects of genetic changes on cells, especially gene knock-outs. Here, we provide data on phenotypic analysis of six strains and show that we can detect expected phenotypes as well as, in some cases, unexpected phenotypes. | ||
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| + | !align=left |Links | ||
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| + | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11435407 PubMed] | ||
| + | Online version:[http://dx.doi.org/10.1101/gr.186501 10.1101/gr.186501] | ||
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| + | !align=left |Keywords | ||
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| + | Escherichia coli; Gene Deletion; Gene Targeting; Genes, Bacterial; Mutation; Oligonucleotide Array Sequence Analysis; Phenotype | ||
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| + | <!--box uid=2ccfb3c7bf1208312f02a69e64bfd9e0.1299.C4dbaede7c2ece--> | ||
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| + | ==Main Points of the Paper == | ||
| + | {{LitSignificance}} | ||
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| + | == Materials and Methods Used == | ||
| + | {{LitMaterials}} | ||
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| + | ==Phenotype Annotations== | ||
| + | {{AnnotationTableHelp}} | ||
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| + | {| id="J4dbaede7ecde9" class=" tableEdit PMID_Phenotype_table" | ||
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| + | !|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes | ||
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| + | ==Notes== | ||
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| + | ==References== | ||
| + | {{RefHelp}} | ||
| + | <references/> | ||
Revision as of 11:57, 29 April 2011
| Citation |
Bochner, BR, Gadzinski, P and Panomitros, E (2001) Phenotype microarrays for high-throughput phenotypic testing and assay of gene function.Genome Res. 11:1246-55 |
|---|---|
| Abstract |
The bacterium Escherichia coli is used as a model cellular system to test and validate a new technology called Phenotype MicroArrays (PMs). PM technology is a high-throughput technology for simultaneous testing of a large number of cellular phenotypes. It consists of preconfigured well arrays in which each well tests a different cellular phenotype and an automated instrument that continuously monitors and records the response of the cells in all wells of the arrays. For example, nearly 700 phenotypes of E. coli can be assayed by merely pipetting a cell suspension into seven microplate arrays. PMs can be used to directly assay the effects of genetic changes on cells, especially gene knock-outs. Here, we provide data on phenotypic analysis of six strains and show that we can detect expected phenotypes as well as, in some cases, unexpected phenotypes. |
| Links |
PubMed Online version:10.1101/gr.186501 |
| Keywords |
Escherichia coli; Gene Deletion; Gene Targeting; Genes, Bacterial; Mutation; Oligonucleotide Array Sequence Analysis; Phenotype |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
| edit table |
</protect>
Notes
References
See Help:References for how to manage references in omp dev.
