Difference between revisions of "PMID:1713075"

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{|  id="Y4ccf0c6dbd2c1"  class=" tableEdit PMID_info_table" 
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!align=left  |Citation
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'''Fessia, SL and Griffin, MJ'''  (1991) A method for assaying biofilm capacity on polyurethane-coated slides.''Perit Dial Int'' '''11''':144-6
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!align=left  |Abstract
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Isolates of coagulase-negative Staphylococci (CNS) were examined for their ability to form biofilms on polyurethane-coated slides. These slides provided a smooth plastic coating simulating polymeric plastic surfaces of medical grade catheter tubing. Slides were placed into plastic conical tubes containing tryptic soy broth inoculated with 10(6) bacteria per mL. The tubes were then incubated at 37 degrees for 48 hours. After incubation, 1 of the slides was stained with a fluorescent acridine orange stain and the other with a safranin stain. The incubation tubes were also stained with safranin. Forty-eight percent of the 65 CNS isolates were found to form a biofilm using acridine orange staining. Forty percent of the 65 CNS isolates were found to form a biofilm using the safranin stain on slides, whereas only 34% were found to adhere on sides of plastic tubes. Increased sensitivity of the fluorescent stain was probably due to enhanced visualization of smaller numbers of bacteria on the plastic. This method using fluorescent stained plastic-coated slides was easier to visualize and interpret than the tube method.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1713075 PubMed]
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!align=left  |Keywords
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Acridine Orange; Bacterial Adhesion; Catheters, Indwelling; Humans; Peritonitis; Phenazines; Polyurethanes; Staining and Labeling; Staphylococcal Infections; Staphylococcus; Staphylococcus epidermidis
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==Main Points of the Paper ==
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{{LitSignificance}}
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== Materials and Methods Used ==
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==Phenotype Annotations==
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{|  id="D4ccf0c6dc4277"  class=" tableEdit PMID_Phenotype_table" 
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!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
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==Notes==
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<references/>

Revision as of 13:52, 1 November 2010

Citation

Fessia, SL and Griffin, MJ (1991) A method for assaying biofilm capacity on polyurethane-coated slides.Perit Dial Int 11:144-6

Abstract

Isolates of coagulase-negative Staphylococci (CNS) were examined for their ability to form biofilms on polyurethane-coated slides. These slides provided a smooth plastic coating simulating polymeric plastic surfaces of medical grade catheter tubing. Slides were placed into plastic conical tubes containing tryptic soy broth inoculated with 10(6) bacteria per mL. The tubes were then incubated at 37 degrees for 48 hours. After incubation, 1 of the slides was stained with a fluorescent acridine orange stain and the other with a safranin stain. The incubation tubes were also stained with safranin. Forty-eight percent of the 65 CNS isolates were found to form a biofilm using acridine orange staining. Forty percent of the 65 CNS isolates were found to form a biofilm using the safranin stain on slides, whereas only 34% were found to adhere on sides of plastic tubes. Increased sensitivity of the fluorescent stain was probably due to enhanced visualization of smaller numbers of bacteria on the plastic. This method using fluorescent stained plastic-coated slides was easier to visualize and interpret than the tube method.

Links

PubMed

Keywords

Acridine Orange; Bacterial Adhesion; Catheters, Indwelling; Humans; Peritonitis; Phenazines; Polyurethanes; Staining and Labeling; Staphylococcal Infections; Staphylococcus; Staphylococcus epidermidis

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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

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Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes
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Notes

Retrieved from "http://bcbp-ftq4xm2.biobio.tamu.edu/omp/dev/index.php?title=PMID:1713075&oldid=427"