PMID:15916962

Citation	Bernhardt, TG and de Boer, PA (2005) SlmA, a nucleoid-associated, FtsZ binding protein required for blocking septal ring assembly over Chromosomes in E. coli.Mol. Cell 18:555-64
Abstract	Cell division in Escherichia coli begins with assembly of the tubulin-like FtsZ protein into a ring structure just underneath the cell membrane. Spatial control over Z ring assembly is achieved by two partially redundant negative regulatory systems, the Min system and nucleoid occlusion (NO), which cooperate to position the division site at midcell. In contrast to the well-studied Min system, almost nothing is known about how Z ring assembly is blocked in the vicinity of nucleoids to effect NO. Reasoning that Min function might become essential in cells impaired for NO, we screened for mutations synthetically lethal with a defective Min system (slm mutants). By using this approach, we identified SlmA (Ttk) as the first NO factor in E. coli. Our combined genetic, cytological, and biochemical results suggest that SlmA is a DNA-associated division inhibitor that is directly involved in preventing Z ring assembly on portions of the membrane surrounding the nucleoid.
Links	PubMed Online version:10.1016/j.molcel.2005.04.012
Keywords	Carrier Proteins; Cell Division; Cell Membrane; Chromosomes, Bacterial; DNA Mutational Analysis; Escherichia coli; Escherichia coli Proteins; Recombinant Fusion Proteins
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Main Points of the Paper

• slmA is synthetically lethal with minB
• Nucleoid cutting results in slmA mutants, indicating SlmA has a role in septal ring positioning

Materials and Methods Used

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Phenotype Annotations

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Species	Taxon ID	Strain	Gene (if known)	OMP	Phenotype	Details	Evidence	Notes
Escherichia coli		TB86	slmA minB	filamentation absent septation	Morphology		Microscopy	Figure 1 and 2
Escherichia coli		TB105	slmA	nucleoid cutting/guillotining	Morphology	septal rings form and constrict over regions of DNA	Microscopy	Figure 3
Escherichia coli		TB86	slmA minB	no growth	Morphology	synthetic lethality	Microscopy	Figure 1
Escherichia coli		TB105	slmA	nucleoid free cells	Morphology		Microscopy	Figure 3
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References

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