Difference between revisions of "PMID:4599365"

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{|  id="G4d76b1da78d8a"  class=" tableEdit PMID_info_table" 
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!align=left  |Citation
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'''Goebel, W'''  (1974) Integrative suppression of temperature-sensitive mutants iwth a lesion in the initiation of DNA replication. Replication of autonomous plasmids in the suppressed state.''Eur. J. Biochem.'' '''43''':125-30
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|-
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!align=left  |Abstract
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No abstract in PubMed
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!align=left  |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4599365 PubMed]
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|-
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!align=left  |Keywords
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Centrifugation, Density Gradient; Chromosome Mapping; DNA Replication; Drug Resistance, Microbial; Escherichia coli; Extrachromosomal Inheritance; Gene Frequency; Mutation; Species Specificity; Streptomycin; Suppression, Genetic; Temperature; Thymine; Tritium
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|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=2ccfb3c7bf1208312f02a69e64bfd9e0.335.G4d76b1da78d8a&page=335&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> ||
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<!--box uid=2ccfb3c7bf1208312f02a69e64bfd9e0.335.G4d76b1da78d8a-->
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==Main Points of the Paper ==
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*Hfr strains suppress initiation of DNA replication mutants via integrative suppression, giving rise to temperature-resistant revertants
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*The presence of other plasmids affect the reversion rate in Hfr strains
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**Multiple excisions and integrations may take place- the presence of other plasmids may be blocking proper sites for F factor.
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*F-like but not I-like plasmids support integrative suppression in ''dnaA'' strains defective for initiation of DNA replication, not ''dnaC'' mutants
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*Non-transmissible ColE1 and ColE2 plasmids do not cause integrative suppression
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*Most of the time the entire plasmid is integrated into the chromosome of the suppressed revertants
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**Hemolytic factor integrates only part of the plasmid while the other part remains autonomous
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*Autonomous ColE1 plasmid can only be maintained in limited copies when the chromosome is being replicated under integrative suppression
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*Autonomous R1 factor replication continues under integrative suppression
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== Materials and Methods Used ==
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*[[:Category:Integrative Suppression|Integrative Suppression]]
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*EtBr sensitivity assay
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*Monitor growth rate
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*Thymidine incorporation to measure DNA synthesis rate
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==Phenotype Annotations==
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{{AnnotationTableHelp}}
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{|  id="G4d76b1da7a7b1"  class=" tableEdit PMID_Phenotype_table" 
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|-
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!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
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|-
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CRT-46
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|
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|
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streptomycin resistant
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|
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Resistance to
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|
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|
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Plating Assay
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|
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|-
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|
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|
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|
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CRT-46
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|
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|
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abolished lactose utilization
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|
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Metabolic Activity
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|
 +
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|
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Plating Assay
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|
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 +
|-
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|
 +
 +
|
 +
 +
|
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CRT-46
 +
|
 +
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|
 +
threonine auxotroph
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|
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Growth
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|
 +
 +
|
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Plating Assay
 +
|
 +
 +
|-
 +
|
 +
 +
|
 +
 +
|
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CRT-46
 +
|
 +
 +
|
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abolished lactose utilization
 +
|
 +
Metabolic Activity
 +
|
 +
 +
|
 +
Plating Assay
 +
|
 +
 +
|-
 +
|
 +
 +
|
 +
 +
|
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CRT-46
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|
 +
 +
|
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leucine auxotroph
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|
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Growth
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|
 +
 +
|
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Plating Assay
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|
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|-
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|
 +
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|
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|
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CRT-46
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|
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|
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isoleucine auxotroph
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|
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Growth
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|
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|
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Plating Assay
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|
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|-
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|
 +
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|
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|
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CRT-46
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|
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|
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thiamine auxotroph
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|
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Growth
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|
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|
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Plating Assay
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|
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|-
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|
 +
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|
 +
 +
|
 +
CRT-46
 +
|
 +
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|
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thymine auxotroph
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|
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Growth
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|
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|
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Plating Assay
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|
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|-
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|
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''Escherichia coli''
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|
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|
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Hfr-120/6
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|
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|
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thiamine auxotroph
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|
 +
Growth
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|
 +
 +
|
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Plating Assay
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|
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|-
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|
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''Escherichia coli''
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|
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|
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Hfr-120/6
 +
|
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|
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thymine auxotroph
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|
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Growth
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|
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|
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Plating Assay
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|
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|-
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|
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''Escherichia coli''
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|
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|
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PC2
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|
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|
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thymine auxotroph
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|
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Growth
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|
 +
 +
|
 +
Plating Assay
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|
 +
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
 +
|
 +
PC2
 +
|
 +
 +
|
 +
leucine auxotroph
 +
|
 +
Growth
 +
|
 +
 +
|
 +
Plating Assay
 +
|
 +
 +
|-
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|
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''Escherichia coli''
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|
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|
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PC2
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|
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|
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streptomyin resistant
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|
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|
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|
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|
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|-
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|
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''Escherichia coli''
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|
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NCBI:
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|
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Hfr-120/6
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|
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 +
|
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temperature sensitive defect in initiation of DNA replication
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|
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|
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|
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|
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|- class="tableEdit_footer"
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|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=2ccfb3c7bf1208312f02a69e64bfd9e0.335.G4d76b1da7a7b1&page=335&pagename={{FULLPAGENAMEE}}&type=0&template=PMID_Phenotype_table edit table]</span> || || || || || || || ||
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<!--box uid=2ccfb3c7bf1208312f02a69e64bfd9e0.335.G4d76b1da7a7b1--></protect>
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==Notes==
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==References==
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{{RefHelp}}
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<references/>
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[[Category:Integrative Suppression]]
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[[Category:Publication]]
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[[Category:To Be Converted]]

Latest revision as of 12:09, 22 June 2011

Citation

Goebel, W (1974) Integrative suppression of temperature-sensitive mutants iwth a lesion in the initiation of DNA replication. Replication of autonomous plasmids in the suppressed state.Eur. J. Biochem. 43:125-30

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Centrifugation, Density Gradient; Chromosome Mapping; DNA Replication; Drug Resistance, Microbial; Escherichia coli; Extrachromosomal Inheritance; Gene Frequency; Mutation; Species Specificity; Streptomycin; Suppression, Genetic; Temperature; Thymine; Tritium

edit table

Main Points of the Paper

  • Hfr strains suppress initiation of DNA replication mutants via integrative suppression, giving rise to temperature-resistant revertants
  • The presence of other plasmids affect the reversion rate in Hfr strains
    • Multiple excisions and integrations may take place- the presence of other plasmids may be blocking proper sites for F factor.
  • F-like but not I-like plasmids support integrative suppression in dnaA strains defective for initiation of DNA replication, not dnaC mutants
  • Non-transmissible ColE1 and ColE2 plasmids do not cause integrative suppression
  • Most of the time the entire plasmid is integrated into the chromosome of the suppressed revertants
    • Hemolytic factor integrates only part of the plasmid while the other part remains autonomous
  • Autonomous ColE1 plasmid can only be maintained in limited copies when the chromosome is being replicated under integrative suppression
  • Autonomous R1 factor replication continues under integrative suppression

Materials and Methods Used

  • Integrative Suppression
  • EtBr sensitivity assay
  • Monitor growth rate
  • Thymidine incorporation to measure DNA synthesis rate

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes

CRT-46

streptomycin resistant

Resistance to

Plating Assay

CRT-46

abolished lactose utilization

Metabolic Activity

Plating Assay

CRT-46

threonine auxotroph

Growth

Plating Assay

CRT-46

abolished lactose utilization

Metabolic Activity

Plating Assay

CRT-46

leucine auxotroph

Growth

Plating Assay

CRT-46

isoleucine auxotroph

Growth

Plating Assay

CRT-46

thiamine auxotroph

Growth

Plating Assay

CRT-46

thymine auxotroph

Growth

Plating Assay

Escherichia coli

Hfr-120/6

thiamine auxotroph

Growth

Plating Assay

Escherichia coli

Hfr-120/6

thymine auxotroph

Growth

Plating Assay

Escherichia coli

PC2

thymine auxotroph

Growth

Plating Assay

Escherichia coli

PC2

leucine auxotroph

Growth

Plating Assay

Escherichia coli

PC2

streptomyin resistant

Escherichia coli

NCBI:

Hfr-120/6

temperature sensitive defect in initiation of DNA replication


edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

Retrieved from "http://bcbp-ftq4xm2.biobio.tamu.edu/omp/dev/index.php?title=PMID:4599365&oldid=5207"