Difference between revisions of "PMID:4599365"

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==Main Points of the Paper ==
 
==Main Points of the Paper ==
{{LitSignificance}}
+
*Hfr strains suppress initiation of DNA replication mutants via integrative suppression, giving rise to temperature-resistant revertants
 +
*The presence of other plasmids affect the reversion rate in Hfr strains
 +
**Multiple excisions and integrations may take place- the presence of other plasmids may be blocking proper sites for F factor.
 +
*F-like but not I-like plasmids support integrative suppression in ''dnaA'' strains defective for initiation of DNA replication, not ''dnaC'' mutants
 +
*Non-transmissible ColE1 and ColE2 plasmids do not cause integrative suppression
 +
*Most of the time the entire plasmid is integrated into the chromosome of the suppressed revertants
 +
**Hemolytic factor integrates only part of the plasmid while the other part remains autonomous
 +
*Autonomous ColE1 plasmid can only be maintained in limited copies when the chromosome is being replicated under integrative suppression
 +
*Autonomous R1 factor replication continues under integrative suppression
  
 
== Materials and Methods Used ==
 
== Materials and Methods Used ==
{{LitMaterials}}
+
*[[:Category:Integrative Suppression|Integrative Suppression]]
 +
*EtBr sensitivity assay
 +
*Monitor growth rate
 +
*Thymidine incorporation to measure DNA synthesis rate
  
 
==Phenotype Annotations==
 
==Phenotype Annotations==
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|-
 
|-
 
!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
 
!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
 +
|-
 +
|
 +
 +
|
 +
 +
|
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CRT-46
 +
|
 +
 +
|
 +
streptomycin resistant
 +
|
 +
Resistance to
 +
|
 +
 +
|
 +
Plating Assay
 +
|
 +
 +
|-
 +
|
 +
 +
|
 +
 +
|
 +
CRT-46
 +
|
 +
 +
|
 +
abolished lactose utilization
 +
|
 +
Metabolic Activity
 +
|
 +
 +
|
 +
Plating Assay
 +
|
 +
 +
|-
 +
|
 +
 +
|
 +
 +
|
 +
CRT-46
 +
|
 +
 +
|
 +
threonine auxotroph
 +
|
 +
Growth
 +
|
 +
 +
|
 +
Plating Assay
 +
|
 +
 +
|-
 +
|
 +
 +
|
 +
 +
|
 +
CRT-46
 +
|
 +
 +
|
 +
abolished lactose utilization
 +
|
 +
Metabolic Activity
 +
|
 +
 +
|
 +
Plating Assay
 +
|
 +
 +
|-
 +
|
 +
 +
|
 +
 +
|
 +
CRT-46
 +
|
 +
 +
|
 +
leucine auxotroph
 +
|
 +
Growth
 +
|
 +
 +
|
 +
Plating Assay
 +
|
 +
 +
|-
 +
|
 +
 +
|
 +
 +
|
 +
CRT-46
 +
|
 +
 +
|
 +
isoleucine auxotroph
 +
|
 +
Growth
 +
|
 +
 +
|
 +
Plating Assay
 +
|
 +
 +
|-
 +
|
 +
 +
|
 +
 +
|
 +
CRT-46
 +
|
 +
 +
|
 +
thiamine auxotroph
 +
|
 +
Growth
 +
|
 +
 +
|
 +
Plating Assay
 +
|
 +
 +
|-
 +
|
 +
 +
|
 +
 +
|
 +
CRT-46
 +
|
 +
 +
|
 +
thymine auxotroph
 +
|
 +
Growth
 +
|
 +
 +
|
 +
Plating Assay
 +
|
 +
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
 +
|
 +
Hfr-120/6
 +
|
 +
 +
|
 +
thiamine auxotroph
 +
|
 +
Growth
 +
|
 +
 +
|
 +
Plating Assay
 +
|
 +
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
 +
|
 +
Hfr-120/6
 +
|
 +
 +
|
 +
thymine auxotroph
 +
|
 +
Growth
 +
|
 +
 +
|
 +
Plating Assay
 +
|
 +
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
 +
|
 +
PC2
 +
|
 +
 +
|
 +
thymine auxotroph
 +
|
 +
Growth
 +
|
 +
 +
|
 +
Plating Assay
 +
|
 +
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
 +
|
 +
PC2
 +
|
 +
 +
|
 +
leucine auxotroph
 +
|
 +
Growth
 +
|
 +
 +
|
 +
Plating Assay
 +
|
 +
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
 +
|
 +
PC2
 +
|
 +
 +
|
 +
streptomyin resistant
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
 +
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
NCBI:
 +
|
 +
Hfr-120/6
 +
|
 +
 +
|
 +
temperature sensitive defect in initiation of DNA replication
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
 +
  
 
|- class="tableEdit_footer"  
 
|- class="tableEdit_footer"  
Line 63: Line 340:
 
<references/>
 
<references/>
  
[[Category:Template]]
+
[[Category:Integrative Suppression]]
 +
 
 +
[[Category:Publication]]
 +
[[Category:To Be Converted]]

Latest revision as of 12:09, 22 June 2011

Citation

Goebel, W (1974) Integrative suppression of temperature-sensitive mutants iwth a lesion in the initiation of DNA replication. Replication of autonomous plasmids in the suppressed state.Eur. J. Biochem. 43:125-30

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Centrifugation, Density Gradient; Chromosome Mapping; DNA Replication; Drug Resistance, Microbial; Escherichia coli; Extrachromosomal Inheritance; Gene Frequency; Mutation; Species Specificity; Streptomycin; Suppression, Genetic; Temperature; Thymine; Tritium

Main Points of the Paper

  • Hfr strains suppress initiation of DNA replication mutants via integrative suppression, giving rise to temperature-resistant revertants
  • The presence of other plasmids affect the reversion rate in Hfr strains
    • Multiple excisions and integrations may take place- the presence of other plasmids may be blocking proper sites for F factor.
  • F-like but not I-like plasmids support integrative suppression in dnaA strains defective for initiation of DNA replication, not dnaC mutants
  • Non-transmissible ColE1 and ColE2 plasmids do not cause integrative suppression
  • Most of the time the entire plasmid is integrated into the chromosome of the suppressed revertants
    • Hemolytic factor integrates only part of the plasmid while the other part remains autonomous
  • Autonomous ColE1 plasmid can only be maintained in limited copies when the chromosome is being replicated under integrative suppression
  • Autonomous R1 factor replication continues under integrative suppression

Materials and Methods Used

  • Integrative Suppression
  • EtBr sensitivity assay
  • Monitor growth rate
  • Thymidine incorporation to measure DNA synthesis rate

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes

CRT-46

streptomycin resistant

Resistance to

Plating Assay

CRT-46

abolished lactose utilization

Metabolic Activity

Plating Assay

CRT-46

threonine auxotroph

Growth

Plating Assay

CRT-46

abolished lactose utilization

Metabolic Activity

Plating Assay

CRT-46

leucine auxotroph

Growth

Plating Assay

CRT-46

isoleucine auxotroph

Growth

Plating Assay

CRT-46

thiamine auxotroph

Growth

Plating Assay

CRT-46

thymine auxotroph

Growth

Plating Assay

Escherichia coli

Hfr-120/6

thiamine auxotroph

Growth

Plating Assay

Escherichia coli

Hfr-120/6

thymine auxotroph

Growth

Plating Assay

Escherichia coli

PC2

thymine auxotroph

Growth

Plating Assay

Escherichia coli

PC2

leucine auxotroph

Growth

Plating Assay

Escherichia coli

PC2

streptomyin resistant

Escherichia coli

NCBI:

Hfr-120/6

temperature sensitive defect in initiation of DNA replication


</protect>

Notes

References

See Help:References for how to manage references in omp dev.