Difference between revisions of "PMID:4599365"
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==Main Points of the Paper == | ==Main Points of the Paper == | ||
| − | + | *Hfr strains suppress initiation of DNA replication mutants via integrative suppression, giving rise to temperature-resistant revertants | |
| + | *The presence of other plasmids affect the reversion rate in Hfr strains | ||
| + | **Multiple excisions and integrations may take place- the presence of other plasmids may be blocking proper sites for F factor. | ||
| + | *F-like but not I-like plasmids support integrative suppression in ''dnaA'' strains defective for initiation of DNA replication, not ''dnaC'' mutants | ||
| + | *Non-transmissible ColE1 and ColE2 plasmids do not cause integrative suppression | ||
| + | *Most of the time the entire plasmid is integrated into the chromosome of the suppressed revertants | ||
| + | **Hemolytic factor integrates only part of the plasmid while the other part remains autonomous | ||
| + | *Autonomous ColE1 plasmid can only be maintained in limited copies when the chromosome is being replicated under integrative suppression | ||
| + | *Autonomous R1 factor replication continues under integrative suppression | ||
== Materials and Methods Used == | == Materials and Methods Used == | ||
| − | + | *[[:Category:Integrative Suppression|Integrative Suppression]] | |
| + | *EtBr sensitivity assay | ||
| + | *Monitor growth rate | ||
| + | *Thymidine incorporation to measure DNA synthesis rate | ||
==Phenotype Annotations== | ==Phenotype Annotations== | ||
| Line 59: | Line 70: | ||
CRT-46 | CRT-46 | ||
| | | | ||
| − | + | ||
| | | | ||
streptomycin resistant | streptomycin resistant | ||
| Line 101: | Line 112: | ||
threonine auxotroph | threonine auxotroph | ||
| | | | ||
| − | + | Growth | |
| | | | ||
| Line 116: | Line 127: | ||
CRT-46 | CRT-46 | ||
| | | | ||
| − | + | ||
| | | | ||
abolished lactose utilization | abolished lactose utilization | ||
| Line 139: | Line 150: | ||
leucine auxotroph | leucine auxotroph | ||
| | | | ||
| − | + | Growth | |
| | | | ||
| Line 158: | Line 169: | ||
isoleucine auxotroph | isoleucine auxotroph | ||
| | | | ||
| − | + | Growth | |
| | | | ||
| Line 177: | Line 188: | ||
thiamine auxotroph | thiamine auxotroph | ||
| | | | ||
| − | + | Growth | |
| | | | ||
| Line 196: | Line 207: | ||
thymine auxotroph | thymine auxotroph | ||
| | | | ||
| − | + | Growth | |
| | | | ||
| Line 207: | Line 218: | ||
''Escherichia coli'' | ''Escherichia coli'' | ||
| | | | ||
| − | + | ||
| | | | ||
Hfr-120/6 | Hfr-120/6 | ||
| Line 213: | Line 224: | ||
| | | | ||
| − | + | thiamine auxotroph | |
| | | | ||
| − | + | Growth | |
| | | | ||
| | | | ||
| − | + | Plating Assay | |
| | | | ||
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| | | | ||
| − | + | thymine auxotroph | |
| | | | ||
| − | + | Growth | |
| | | | ||
| | | | ||
| − | + | Plating Assay | |
| | | | ||
| Line 247: | Line 258: | ||
| | | | ||
| − | + | PC2 | |
| | | | ||
| Line 253: | Line 264: | ||
thymine auxotroph | thymine auxotroph | ||
| | | | ||
| − | + | Growth | |
| | | | ||
| | | | ||
| − | + | Plating Assay | |
| | | | ||
| Line 270: | Line 281: | ||
| | | | ||
| − | + | leucine auxotroph | |
| | | | ||
| − | + | Growth | |
| | | | ||
| | | | ||
| − | + | Plating Assay | |
| | | | ||
| Line 289: | Line 300: | ||
| | | | ||
| − | + | streptomyin resistant | |
| | | | ||
| Line 302: | Line 313: | ||
''Escherichia coli'' | ''Escherichia coli'' | ||
| | | | ||
| − | + | NCBI: | |
| | | | ||
| − | + | Hfr-120/6 | |
| | | | ||
| | | | ||
| − | + | temperature sensitive defect in initiation of DNA replication | |
| | | | ||
| Line 329: | Line 340: | ||
<references/> | <references/> | ||
| − | [[Category: | + | [[Category:Integrative Suppression]] |
| + | |||
| + | [[Category:Publication]] | ||
| + | [[Category:To Be Converted]] | ||
Latest revision as of 12:09, 22 June 2011
| Citation |
Goebel, W (1974) Integrative suppression of temperature-sensitive mutants iwth a lesion in the initiation of DNA replication. Replication of autonomous plasmids in the suppressed state.Eur. J. Biochem. 43:125-30 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Centrifugation, Density Gradient; Chromosome Mapping; DNA Replication; Drug Resistance, Microbial; Escherichia coli; Extrachromosomal Inheritance; Gene Frequency; Mutation; Species Specificity; Streptomycin; Suppression, Genetic; Temperature; Thymine; Tritium |
| edit table |
Main Points of the Paper
- Hfr strains suppress initiation of DNA replication mutants via integrative suppression, giving rise to temperature-resistant revertants
- The presence of other plasmids affect the reversion rate in Hfr strains
- Multiple excisions and integrations may take place- the presence of other plasmids may be blocking proper sites for F factor.
- F-like but not I-like plasmids support integrative suppression in dnaA strains defective for initiation of DNA replication, not dnaC mutants
- Non-transmissible ColE1 and ColE2 plasmids do not cause integrative suppression
- Most of the time the entire plasmid is integrated into the chromosome of the suppressed revertants
- Hemolytic factor integrates only part of the plasmid while the other part remains autonomous
- Autonomous ColE1 plasmid can only be maintained in limited copies when the chromosome is being replicated under integrative suppression
- Autonomous R1 factor replication continues under integrative suppression
Materials and Methods Used
- Integrative Suppression
- EtBr sensitivity assay
- Monitor growth rate
- Thymidine incorporation to measure DNA synthesis rate
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
CRT-46 |
streptomycin resistant |
Resistance to |
Plating Assay |
|||||
|
CRT-46 |
abolished lactose utilization |
Metabolic Activity |
Plating Assay |
|||||
|
CRT-46 |
threonine auxotroph |
Growth |
Plating Assay |
|||||
|
CRT-46 |
abolished lactose utilization |
Metabolic Activity |
Plating Assay |
|||||
|
CRT-46 |
leucine auxotroph |
Growth |
Plating Assay |
|||||
|
CRT-46 |
isoleucine auxotroph |
Growth |
Plating Assay |
|||||
|
CRT-46 |
thiamine auxotroph |
Growth |
Plating Assay |
|||||
|
CRT-46 |
thymine auxotroph |
Growth |
Plating Assay |
|||||
|
Escherichia coli |
Hfr-120/6 |
thiamine auxotroph |
Growth |
Plating Assay |
||||
|
Escherichia coli |
Hfr-120/6 |
thymine auxotroph |
Growth |
Plating Assay |
||||
|
Escherichia coli |
PC2 |
thymine auxotroph |
Growth |
Plating Assay |
||||
|
Escherichia coli |
PC2 |
leucine auxotroph |
Growth |
Plating Assay |
||||
|
Escherichia coli |
PC2 |
streptomyin resistant |
||||||
|
Escherichia coli |
NCBI: |
Hfr-120/6 |
temperature sensitive defect in initiation of DNA replication |
| ||||
| edit table |
</protect>
Notes
References
See Help:References for how to manage references in omp dev.
