Difference between revisions of "PMID:12769856"
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*NCBI Taxon ID: 83333 | *NCBI Taxon ID: 83333 | ||
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| − | *Genotype of Reference Strain: ''umuD'C'' | + | *Genotype of Reference Strain: ''umuD'C''D34W |
| − | *Genotype of Experimental Strain : ''umuD'C'' | + | *Genotype of Experimental Strain : ''umuD'C''D34A |
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*Reference Condition: | *Reference Condition: | ||
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*NCBI Taxon ID: 83333 | *NCBI Taxon ID: 83333 | ||
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| − | *Genotype of Reference Strain: ''umuD'C'' | + | *Genotype of Reference Strain: ''umuD'C''N33W |
| − | *Genotype of Experimental Strain : ''umuD'C'' | + | *Genotype of Experimental Strain : ''umuD'C''N33A |
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*Reference Condition: | *Reference Condition: | ||
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| − | By mutating the amino acid ala at the | + | By mutating the amino acid ala at the 33 position, the ''umuD'C'' is deleted, causing hypersensitivity to UV radiation. |
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Revision as of 17:45, 27 January 2012
| Citation |
Morimatsu, K and Kowalczykowski, SC (2003) RecFOR proteins load RecA protein onto gapped DNA to accelerate DNA strand exchange: a universal step of recombinational repair.Mol. Cell 11:1337-47 |
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| Abstract |
Genetic evidence suggests that the RecF, RecO, and RecR (RecFOR) proteins participate in a common step of DNA recombination and repair, yet the biochemical event requiring collaboration of all three proteins is unknown. Here, we show that the concerted action of the RecFOR complex directs the loading of RecA protein specifically onto gapped DNA that is coated with single-stranded DNA binding (SSB) protein, thereby accelerating DNA strand exchange. The RecFOR complex recognizes the junction between the ssDNA and dsDNA regions and requires a base-paired 5' terminus at the junction. Thus, the RecFOR complex is a structure-specific mediator that targets recombinational repair to ssDNA-dsDNA junctions. This reaction reconstitutes the initial steps of recombinational gapped DNA repair and uncovers an event also common to the repair of ssDNA-tailed intermediates of dsDNA-break repair. We propose that the behavior of the RecFOR proteins is mimicked by functional counterparts that exist in all organisms. |
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| Keywords |
Bacterial Proteins; Cell Nucleus; DNA; DNA Damage; DNA Repair; DNA, Single-Stranded; DNA-Binding Proteins; Escherichia coli Proteins; Eukaryotic Cells; Gene Rearrangement; Nucleoproteins; Prokaryotic Cells; Rec A Recombinases |
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Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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a mutation or genetic difference within a strain |
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By mutating the amino acid ala at the 32 position, the umuD'C is deleted, causing hypersensitivity to UV radiation. |
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a mutation or genetic difference within a strain |
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By mutating the amino acid ala at the 34 position, the umuD'C is deleted, causing hypersensitivity to UV radiation. |
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a mutation or genetic difference within a strain |
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By mutating the amino acid ala at the 33 position, the umuD'C is deleted, causing hypersensitivity to UV radiation. |
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</protect>
Notes
References
See Help:References for how to manage references in omp dev.
