Difference between revisions of "PMID:128555"

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'''Mizuno, T, Yamada, H, Yamagata, H and Mizushima, S'''  (1976) Coordinated alterations in ribosomes and cytoplasmic membrane in sucrose-dependent, spectinomycin-resistant mutants of Escherichia coli.''J. Bacteriol.'' '''125''':524-30
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!align=left  |Abstract
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Alterations in cytoplasmic membrane and ribosomes from sucrose-dependent spectinomycin-resistant (Sucd-Spcr) mutants of Escherichia coli, mutants that are resistant to spectinomycin in the presence of 20% sucrose but sensitive in the absence of sucrose, were studied. The protein composition of cytoplasmic membrane was analyzed by gel electrophoresis on polyacrylamide gel containing 8 M urea and 0.5% sodium dodecyl sulfate, which assured the reproducible separation of 28 protein bands. A major protein band, I-19, was missing in all cytoplasmic membrane preparations from 10 Sucd-Spcr mutants. Besides protein I-19, proteins I-13 and I-24 were missing in some mutants. On the other hand, the protein composition of cytoplasmic membrane from a sucrose-independent spectinomycin-resistant mutant was indistinguishable from that from the wild-type strain. The polypeptide synthetic activity of ribosomes from Sucd-Spcr mutants was resistant to spectinomycin. Studies on a revertant obtained from one of these mutants without any selection for sensitivity to spectinomycin revealed that a single mutation was responsible for both the ribosomal alteration, i.e., spectinomycin resistance, and the lack of protein I-19 in the cytoplasmic membrane. Studies on a transductant obtained with a Sucd-SPcr mutant as the donor also confirmed the single-mutation concept. It was concluded that in Sucd-SPcr mutants an alteration in the ribosomes caused the deletion of protein I-19 from cytoplasmic membrane.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=128555 PubMed]
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!align=left  |Keywords
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Bacterial Proteins; Cell Membrane; Cell-Free System; Drug Resistance, Microbial; Escherichia coli; Mutation; Peptide Biosynthesis; Ribosomes; Spectinomycin; Sucrose
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==Main Points of the Paper ==
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{{LitSignificance}}
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== Materials and Methods Used ==
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{{LitMaterials}}
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==Phenotype Annotations==
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{|  id="E4f641431a4818"  class=" tableEdit Phenotype_Table_2" 
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: YM50
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*NCBI Taxon ID: 83333
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*Genotype of Reference Strain: Suc<sup>+</sup>-Spc<sup>+</sup>
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*Genotype of Experimental Strain : Suc<sup>d</sup>-Spc<sup>r</sup> in YM50
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*Reference Condition:
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The ribosomal alteration that causes spectinomycin also caused the protein I-19 to be deleted.  This was also detected in strains YM22, YM26, YM37, YM54, YM69, YM84, YM85, YM93 & YM101. See table 2.
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: YM101
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*NCBI Taxon ID: 83333
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*Genotype of Reference Strain: Suc<sup>+</sup>-Spc<sup>+</sup>
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*Genotype of Experimental Strain : Suc<sup>d</sup>-Spc<sup>r</sup> in YM101
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*Reference Condition:
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The ribosomal alteration that causes spectinomycin also caused the protein I-13 to be deleted. See table 2 and Figure 1.
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: YM22
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*NCBI Taxon ID: 83333
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*Genotype of Reference Strain: Suc<sup>+</sup>-Spc<sup>+</sup>
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*Genotype of Experimental Strain : Suc<sup>d</sup>-Spc<sup>r</sup> in YM22
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*Reference Condition:
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The ribosomal alteration that causes spectinomycin also caused the protein I-24 to be deleted. I-24 was deleted in stains YM50 & YM93. See table 2.
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==Notes==
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==References==
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[[Category:Publication]]

Latest revision as of 00:28, 17 March 2012

Citation

Mizuno, T, Yamada, H, Yamagata, H and Mizushima, S (1976) Coordinated alterations in ribosomes and cytoplasmic membrane in sucrose-dependent, spectinomycin-resistant mutants of Escherichia coli.J. Bacteriol. 125:524-30

Abstract

Alterations in cytoplasmic membrane and ribosomes from sucrose-dependent spectinomycin-resistant (Sucd-Spcr) mutants of Escherichia coli, mutants that are resistant to spectinomycin in the presence of 20% sucrose but sensitive in the absence of sucrose, were studied. The protein composition of cytoplasmic membrane was analyzed by gel electrophoresis on polyacrylamide gel containing 8 M urea and 0.5% sodium dodecyl sulfate, which assured the reproducible separation of 28 protein bands. A major protein band, I-19, was missing in all cytoplasmic membrane preparations from 10 Sucd-Spcr mutants. Besides protein I-19, proteins I-13 and I-24 were missing in some mutants. On the other hand, the protein composition of cytoplasmic membrane from a sucrose-independent spectinomycin-resistant mutant was indistinguishable from that from the wild-type strain. The polypeptide synthetic activity of ribosomes from Sucd-Spcr mutants was resistant to spectinomycin. Studies on a revertant obtained from one of these mutants without any selection for sensitivity to spectinomycin revealed that a single mutation was responsible for both the ribosomal alteration, i.e., spectinomycin resistance, and the lack of protein I-19 in the cytoplasmic membrane. Studies on a transductant obtained with a Sucd-SPcr mutant as the donor also confirmed the single-mutation concept. It was concluded that in Sucd-SPcr mutants an alteration in the ribosomes caused the deletion of protein I-19 from cytoplasmic membrane.

Links

PubMed

Keywords

Bacterial Proteins; Cell Membrane; Cell-Free System; Drug Resistance, Microbial; Escherichia coli; Mutation; Peptide Biosynthesis; Ribosomes; Spectinomycin; Sucrose

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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: YM50
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: Suc+-Spc+
  • Genotype of Experimental Strain : Sucd-Spcr in YM50
  • Reference Condition:

The ribosomal alteration that causes spectinomycin also caused the protein I-19 to be deleted. This was also detected in strains YM22, YM26, YM37, YM54, YM69, YM84, YM85, YM93 & YM101. See table 2.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: YM101
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: Suc+-Spc+
  • Genotype of Experimental Strain : Sucd-Spcr in YM101
  • Reference Condition:

The ribosomal alteration that causes spectinomycin also caused the protein I-13 to be deleted. See table 2 and Figure 1.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: YM22
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: Suc+-Spc+
  • Genotype of Experimental Strain : Sucd-Spcr in YM22
  • Reference Condition:

The ribosomal alteration that causes spectinomycin also caused the protein I-24 to be deleted. I-24 was deleted in stains YM50 & YM93. See table 2.


edit table

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Notes

References

See Help:References for how to manage references in omp dev.

Retrieved from "http://bcbp-ftq4xm2.biobio.tamu.edu/omp/dev/index.php?title=PMID:128555&oldid=6531"