PMID:387748

Citation	Dabbs, ER (1979) Selection for Escherichia coli mutants with proteins missing from the ribosome.J. Bacteriol. 140:734-7
Abstract	Antibiotic-independent revertants of an erythromycin-dependent strain of Escherichia coli were isolated by spontaneous selection. Their ribosomal proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis. In contrast to most ribosomally targeted selections, the specific absence of a certain protein from the ribosome, rather than alterations in ribosomal proteins, was observed. Mutants were found with protein S20, L11, L15, L28, L29, or L30 missing.
Links	PubMed
Keywords	Bacterial Proteins; Escherichia coli; Genes; Mutation; Ribosomal Proteins
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: A19 NCBI Taxon ID: 83333	Genotype of Reference Strain: A19 ErmDep Genotype of Experimental Strain : A19 Erm DrpsT	Reference Condition:					It was found that the S20 ribosomal protein was missing indicating that there was a loss of dependence on Erythromycin. It is unknown whether this caused renewed sensitivity toward Erthyromycin. List of mutants include AM13, AM30, AM101, AM106 & AM107 (table 1). See figure 1.
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