Difference between revisions of "PMID:387748"

From omp dev
Jump to: navigation, search
(New PMID: Page!)
 
(Table edited by Wameza via TableEdit)
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
 +
{{RightTOC}}
  
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.2900.X4f74de234d4b8-->
 +
<!--
 +
******************************************************************************************
 +
*
 +
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
 +
*
 +
****************************************************************************************** -->
 +
{|  id="X4f74de234d4b8"  class=" tableEdit PMID_info_table" 
 +
 +
|-
 +
!align=left  |Citation
 +
||
 +
'''Dabbs, ER'''  (1979) Selection for Escherichia coli mutants with proteins missing from the ribosome.''J. Bacteriol.'' '''140''':734-7
 +
|-
 +
!align=left  |Abstract
 +
||
 +
Antibiotic-independent revertants of an erythromycin-dependent strain of Escherichia coli were isolated by spontaneous selection. Their ribosomal proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis. In contrast to most ribosomally targeted selections, the specific absence of a certain protein from the ribosome, rather than alterations in ribosomal proteins, was observed. Mutants were found with protein S20, L11, L15, L28, L29, or L30 missing.
 +
|-
 +
!align=left  |Links
 +
||
 +
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=387748 PubMed]
 +
 +
|-
 +
!align=left  |Keywords
 +
||
 +
Bacterial Proteins; Escherichia coli; Genes; Mutation; Ribosomal Proteins
 +
 +
|- class="tableEdit_footer"
 +
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.2900.X4f74de234d4b8&page=2900&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> ||
 +
|}
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.2900.X4f74de234d4b8-->
 +
 +
==Main Points of the Paper ==
 +
{{LitSignificance}}
 +
 +
== Materials and Methods Used ==
 +
{{LitMaterials}}
 +
 +
==Phenotype Annotations==
 +
{{AnnotationTableHelp}}
 +
<protect><!--box uid=d41d8cd98f00b204e9800998ecf8427e.2900.S4f74de2356f10-->
 +
<!--
 +
******************************************************************************************
 +
*
 +
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
 +
*
 +
****************************************************************************************** -->
 +
{|  id="S4f74de2356f10"  class=" tableEdit Phenotype_Table_2" 
 +
|-
 +
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: A19
 +
*NCBI Taxon ID: 83333
 +
|
 +
*Genotype of Reference Strain: A19 Erm<sup>Dep</sup>
 +
*Genotype of Experimental Strain : A19 Erm <sup>D</sup>rpsT
 +
|
 +
*Reference Condition:
 +
|
 +
 +
|
 +
 +
|
 +
0000004
 +
|
 +
cell fractionation data
 +
|
 +
It was found that the S20 ribosomal protein was missing indicating that there was a loss of dependence on Erythromycin. It is unknown whether this caused renewed sensitivity toward Erthyromycin.  List of mutants include AM13, AM30, AM101, AM106 & AM107 (table 1). See figure 1. Analysis was done using 2D-page polyacrylamide gels
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: A19
 +
*NCBI Taxon ID: 83333
 +
|
 +
*Genotype of Reference Strain: A19 Erm<sup>Dep</sup>
 +
*Genotype of Experimental Strain : A19 Erm <sup>D</sup>rplK
 +
|
 +
*Reference Condition:
 +
|
 +
 +
|
 +
 +
|
 +
0000004
 +
|
 +
cell fractionation data
 +
|
 +
It was found that the L11 ribosomal protein was missing indicating that there was a loss of translation dependence on Erythromycin. It is unknown whether this caused renewed sensitivity toward Erthyromycin. Mutants include AM68, AM76 and AM77 (table 1.) Analysis was done using 2D-page polyacrylamide gels, See figure 1.
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: A19
 +
*NCBI Taxon ID: 83333
 +
|
 +
*Genotype of Reference Strain: A19 Erm<sup>Dep</sup>
 +
*Genotype of Experimental Strain : A19 Erm <sup>D</sup>rplO
 +
|
 +
*Reference Condition:
 +
|
 +
 +
|
 +
 +
|
 +
0000004
 +
|
 +
cell fractionation data
 +
|
 +
It was found that the L15 ribosomal protein was missing indicating that there was a loss of translation dependence on Erythromycin. It is unknown whether this caused renewed sensitivity toward Erthyromycin. There was one mutant, AM16(Table 1). Analysis was done by 2D-page polyacrylamide gels See Figure 1.
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: A19
 +
*NCBI Taxon ID: 83333
 +
|
 +
*Genotype of Reference Strain: A19 Erm<sup>Dep</sup>
 +
*Genotype of Experimental Strain : A19 Erm <sup>D</sup>rpmB
 +
|
 +
*Reference Condition:
 +
|
 +
 +
|
 +
 +
|
 +
0000004
 +
|
 +
cell fractionation data
 +
|
 +
It was found that the L28 ribosomal protein was missing indicating that there was a loss of translation dependence on Erythromycin. It is unknown whether this caused renewed sensitivity toward Erthyromycin. Mutants include AM3, AM21, AM81 and AM108 (table 1). Analysis was done using 2D-page polyacrylamide gels. See Figure 1
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: A19
 +
*NCBI Taxon ID: 83333
 +
|
 +
*Genotype of Reference Strain: A19 Erm<sup>Dep</sup>
 +
*Genotype of Experimental Strain : A19 Erm <sup>D</sup>rpmC
 +
|
 +
*Reference Condition:
 +
|
 +
 +
|
 +
 +
|
 +
0000004
 +
|
 +
cell fractionation data
 +
|
 +
It was found that the L29 ribosomal protein was missing indicating that there was a loss of translation dependence on Erythromycin. It is unknown whether this caused renewed sensitivity toward Erthyromycin. A known mutant is AM111(see table 1). Analysis was done using 2D-page polyacrylamide gels. See figure 1
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: A19
 +
*NCBI Taxon ID: 83333
 +
|
 +
*Genotype of Reference Strain: A19 Erm<sup>Dep</sup>
 +
*Genotype of Experimental Strain : A19 Erm <sup>D</sup>rpmD
 +
|
 +
*Reference Condition:
 +
|
 +
 +
|
 +
 +
|
 +
0000004
 +
|
 +
cell fractionation data
 +
|
 +
It was found that the L30 ribosomal protein was missing indicating that there was a loss of translation dependence on Erythromycin. It is unknown whether this caused renewed sensitivity toward Erthyromycin. There were 3 mutants AM10, AM46, AM98 (see table 1.) Analysis was done using 2D-page polyacrylamide gels. See Figure 1.
 +
|
 +
 +
 +
|- class="tableEdit_footer"
 +
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.2900.S4f74de2356f10&page=2900&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</span> || || || || || || || || ||
 +
|}
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.2900.S4f74de2356f10--></protect>
 +
 +
==Notes==
 +
 +
==References==
 +
{{RefHelp}}
 +
<references/>
 +
 +
 +
[[Category:Publication]]

Latest revision as of 13:49, 2 April 2012

Citation

Dabbs, ER (1979) Selection for Escherichia coli mutants with proteins missing from the ribosome.J. Bacteriol. 140:734-7

Abstract

Antibiotic-independent revertants of an erythromycin-dependent strain of Escherichia coli were isolated by spontaneous selection. Their ribosomal proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis. In contrast to most ribosomally targeted selections, the specific absence of a certain protein from the ribosome, rather than alterations in ribosomal proteins, was observed. Mutants were found with protein S20, L11, L15, L28, L29, or L30 missing.

Links

PubMed

Keywords

Bacterial Proteins; Escherichia coli; Genes; Mutation; Ribosomal Proteins

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: A19
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: A19 ErmDep
  • Genotype of Experimental Strain : A19 Erm DrpsT
  • Reference Condition:

0000004

cell fractionation data

It was found that the S20 ribosomal protein was missing indicating that there was a loss of dependence on Erythromycin. It is unknown whether this caused renewed sensitivity toward Erthyromycin. List of mutants include AM13, AM30, AM101, AM106 & AM107 (table 1). See figure 1. Analysis was done using 2D-page polyacrylamide gels

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: A19
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: A19 ErmDep
  • Genotype of Experimental Strain : A19 Erm DrplK
  • Reference Condition:

0000004

cell fractionation data

It was found that the L11 ribosomal protein was missing indicating that there was a loss of translation dependence on Erythromycin. It is unknown whether this caused renewed sensitivity toward Erthyromycin. Mutants include AM68, AM76 and AM77 (table 1.) Analysis was done using 2D-page polyacrylamide gels, See figure 1.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: A19
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: A19 ErmDep
  • Genotype of Experimental Strain : A19 Erm DrplO
  • Reference Condition:

0000004

cell fractionation data

It was found that the L15 ribosomal protein was missing indicating that there was a loss of translation dependence on Erythromycin. It is unknown whether this caused renewed sensitivity toward Erthyromycin. There was one mutant, AM16(Table 1). Analysis was done by 2D-page polyacrylamide gels See Figure 1.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: A19
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: A19 ErmDep
  • Genotype of Experimental Strain : A19 Erm DrpmB
  • Reference Condition:

0000004

cell fractionation data

It was found that the L28 ribosomal protein was missing indicating that there was a loss of translation dependence on Erythromycin. It is unknown whether this caused renewed sensitivity toward Erthyromycin. Mutants include AM3, AM21, AM81 and AM108 (table 1). Analysis was done using 2D-page polyacrylamide gels. See Figure 1

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: A19
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: A19 ErmDep
  • Genotype of Experimental Strain : A19 Erm DrpmC
  • Reference Condition:

0000004

cell fractionation data

It was found that the L29 ribosomal protein was missing indicating that there was a loss of translation dependence on Erythromycin. It is unknown whether this caused renewed sensitivity toward Erthyromycin. A known mutant is AM111(see table 1). Analysis was done using 2D-page polyacrylamide gels. See figure 1

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: A19
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: A19 ErmDep
  • Genotype of Experimental Strain : A19 Erm DrpmD
  • Reference Condition:

0000004

cell fractionation data

It was found that the L30 ribosomal protein was missing indicating that there was a loss of translation dependence on Erythromycin. It is unknown whether this caused renewed sensitivity toward Erthyromycin. There were 3 mutants AM10, AM46, AM98 (see table 1.) Analysis was done using 2D-page polyacrylamide gels. See Figure 1.


edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

Retrieved from "http://bcbp-ftq4xm2.biobio.tamu.edu/omp/dev/index.php?title=PMID:387748&oldid=6716"