PMID:6394957

Citation	Boeke, JD, LaCroute, F and Fink, GR (1984) A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.Mol. Gen. Genet. 197:345-6
Abstract	Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3- mutants grow normally. This selection, based on the loss of orotidine-5'-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells.
Links	PubMed
Keywords	Carboxy-Lyases; Drug Resistance, Microbial; Fungal Proteins; Microbiological Techniques; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Saccharomyces cerevisiae
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: DB6507 NCBI Taxon ID: 83333	Genotype of Reference Strain: pyrF Genotype of Experimental Strain : pyrF	Reference Condition:					Resistant to Fluoro(5)orotic acid
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