PMID:4555418

Citation	Venkateswaran, PS and Wu, HC (1972) Isolation and characterization of a phosphonomycin-resistant mutant of Escherichia coli K-12.J. Bacteriol. 110:935-44
Abstract	A mutant was isolated from Escherichia coli K-12 which showed increased resistance towards phosphonomycin, a new bactericidal antibiotic recently isolated from strains of Streptomyces. Evidence is presented which suggests that this mutant is resistant to lysis by phosphonomycin because of a lower affinity of phosphoenolpyruvate: uridine diphospho-N-acetylglucosamine enolpyruvyl transferase for this antibiotic. This mutant was also found to be temperature-sensitive in growth. At 42 C mutant cells grew poorly, and the rate of incorporation of (3)H-diaminopimelic acid into trichloroacetic acid-insoluble material was also greatly reduced. Genetic studies indicate that the increased resistance toward phosphonomycin and temperature sensitivity in growth of this mutant are probably the consequences of a single mutation.
Links	PubMed
Keywords	Anti-Bacterial Agents; Carbon Isotopes; Cell Wall; Chromatography, Paper; Culture Media; Drug Resistance, Microbial; Escherichia coli; Genetics, Microbial; Glucose; Glucosephosphates; Glycerophosphates; Microscopy, Phase-Contrast; Mutation; Phosphoenolpyruvate; Phosphotransferases; Pimelic Acids; Temperature; Tritium
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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: glpT Genotype of Experimental Strain : glpT -	Reference Condition:	OMP:0000276	increased resistance to antibiotics	ECO:0000182	in vitro culture assay data	Phosphonomycin resistant
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: uhp Genotype of Experimental Strain : uhp -	Reference Condition:	OMP:0000276	increased resistance to antibiotics	ECO:0000182	in vitro culture assay data	Phosphonomycin resistant
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: ctr Genotype of Experimental Strain : ctr -	Reference Condition:	OMP:0000276	increased resistance to antibiotics	ECO:0000182	in vitro culture assay data	Phosphonomycin resistant
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