Difference between revisions of "PMID:7050084"

From omp dev
Jump to: navigation, search
(New PMID: Page!)
 
(→‎References)
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
 +
{{RightTOC}}
  
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.2911.P4f8324326d978-->
 +
<!--
 +
******************************************************************************************
 +
*
 +
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
 +
*
 +
****************************************************************************************** -->
 +
{|  id="P4f8324326d978"  class=" tableEdit PMID_info_table" 
 +
 +
|-
 +
!align=left  |Citation
 +
||
 +
'''Böck, A, Turnowsky, F and Högenauer, G'''  (1982) Tiamulin resistance mutations in Escherichia coli.''J. Bacteriol.'' '''151''':1253-60
 +
|-
 +
!align=left  |Abstract
 +
||
 +
Forty "two-step" and 13 "three-step" tiamulin-resistant mutants of Escherichia coli PR11 were isolated and tested for alteration of ribosomal proteins. Mutants with altered ribosomal proteins S10, S19, L3, and L4 were detected. The S19, L3, and L4 mutants were studied in detail. The L3 and L4 mutations did not segregate from the resistance character in transductional crosses and therefore seem to be responsible for the resistance. Extracts of these mutants also exhibited an increased in vitro resistance to tiamulin in the polyuridylic acid and phage R17 RNA-dependent polypeptide synthesis systems, and it was demonstrated that this was a property of the 50S subunit. In the case of the S19 mutant, genetic analysis showed segregation between resistance and the S19 alteration and therefore indicated that mutation of a protein other than S19 was responsible for the resistance phenotype. The isolated ribosomes of the S19, L3, and L4 mutants bound radioactive tiamulin with a considerably reduced strength when compared with those of wild-type cells. The association constants were lower by factors ranging from approximately 20 to 200. When heated in the presence of ammonium chloride, these ribosomes partially regained their avidity for tiamulin.
 +
|-
 +
!align=left  |Links
 +
||
 +
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7050084 PubMed]
 +
 +
|-
 +
!align=left  |Keywords
 +
||
 +
Bacterial Proteins; Diterpenes; Drug Resistance, Microbial; Escherichia coli; Genes, Bacterial; Mutation; Ribosomal Proteins; Ribosomes; Transduction, Genetic
 +
 +
|- class="tableEdit_footer"
 +
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.2911.P4f8324326d978&page=2911&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> ||
 +
|}
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.2911.P4f8324326d978-->
 +
 +
==Main Points of the Paper ==
 +
{{LitSignificance}}
 +
 +
== Materials and Methods Used ==
 +
{{LitMaterials}}
 +
 +
==Phenotype Annotations==
 +
{{AnnotationTableHelp}}
 +
<protect><!--box uid=d41d8cd98f00b204e9800998ecf8427e.2911.X4f8324327b0d5-->
 +
<!--
 +
******************************************************************************************
 +
*
 +
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
 +
*
 +
****************************************************************************************** -->
 +
{|  id="X4f8324327b0d5"  class=" tableEdit Phenotype_Table_2" 
 +
|-
 +
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: TS14-13-10
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&p=mapview&p=has_linkout&p=blast_url&p=genome_blast&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''rpsS''
 +
*Genotype of Experimental Strain : ''rpsS''
 +
|
 +
*Reference Condition:
 +
|
 +
OMP:0000276
 +
|
 +
increased resistance to antibiotics
 +
|
 +
ECO:0000182
 +
|
 +
in vitro culture assay data
 +
|
 +
Three step tiamulin resistance, table 1 and 2
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: TS15-23
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&p=mapview&p=has_linkout&p=blast_url&p=genome_blast&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''rplD''
 +
*Genotype of Experimental Strain : ''rplD''
 +
|
 +
*Reference Condition:
 +
|
 +
OMP:0000276
 +
|
 +
increased resistance to antibiotics
 +
|
 +
ECO:0000182
 +
|
 +
in vitro culture assay data
 +
|
 +
Two step Tiamulin resistant, table 1 and 2
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: TS10-21
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&p=mapview&p=has_linkout&p=blast_url&p=genome_blast&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''rplC''
 +
*Genotype of Experimental Strain : ''rplC''
 +
|
 +
*Reference Condition:
 +
|
 +
OMP:0000276
 +
|
 +
increased resistance to antibiotics
 +
|
 +
ECO:0000182
 +
|
 +
in vitro culture assay data
 +
|
 +
rplC(L3) two step tiamulin resistant in strain 1 and 2
 +
|
 +
 +
 +
|- class="tableEdit_footer"
 +
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.2911.X4f8324327b0d5&page=2911&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</span> || || || || || || || || ||
 +
|}
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.2911.X4f8324327b0d5--></protect>
 +
 +
==Notes==
 +
 +
==References==
 +
{{RefHelp}}
 +
<references/>
 +
 +
 +
[[Category:Publication]] [[Category:Papers referenced in the LaRossa chapter]]

Latest revision as of 16:58, 30 April 2012

Citation

Böck, A, Turnowsky, F and Högenauer, G (1982) Tiamulin resistance mutations in Escherichia coli.J. Bacteriol. 151:1253-60

Abstract

Forty "two-step" and 13 "three-step" tiamulin-resistant mutants of Escherichia coli PR11 were isolated and tested for alteration of ribosomal proteins. Mutants with altered ribosomal proteins S10, S19, L3, and L4 were detected. The S19, L3, and L4 mutants were studied in detail. The L3 and L4 mutations did not segregate from the resistance character in transductional crosses and therefore seem to be responsible for the resistance. Extracts of these mutants also exhibited an increased in vitro resistance to tiamulin in the polyuridylic acid and phage R17 RNA-dependent polypeptide synthesis systems, and it was demonstrated that this was a property of the 50S subunit. In the case of the S19 mutant, genetic analysis showed segregation between resistance and the S19 alteration and therefore indicated that mutation of a protein other than S19 was responsible for the resistance phenotype. The isolated ribosomes of the S19, L3, and L4 mutants bound radioactive tiamulin with a considerably reduced strength when compared with those of wild-type cells. The association constants were lower by factors ranging from approximately 20 to 200. When heated in the presence of ammonium chloride, these ribosomes partially regained their avidity for tiamulin.

Links

PubMed

Keywords

Bacterial Proteins; Diterpenes; Drug Resistance, Microbial; Escherichia coli; Genes, Bacterial; Mutation; Ribosomal Proteins; Ribosomes; Transduction, Genetic

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: TS14-13-10
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: rpsS
  • Genotype of Experimental Strain : rpsS
  • Reference Condition:

OMP:0000276

increased resistance to antibiotics

ECO:0000182

in vitro culture assay data

Three step tiamulin resistance, table 1 and 2

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: TS15-23
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: rplD
  • Genotype of Experimental Strain : rplD
  • Reference Condition:

OMP:0000276

increased resistance to antibiotics

ECO:0000182

in vitro culture assay data

Two step Tiamulin resistant, table 1 and 2

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: TS10-21
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: rplC
  • Genotype of Experimental Strain : rplC
  • Reference Condition:

OMP:0000276

increased resistance to antibiotics

ECO:0000182

in vitro culture assay data

rplC(L3) two step tiamulin resistant in strain 1 and 2


edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

Retrieved from "http://bcbp-ftq4xm2.biobio.tamu.edu/omp/dev/index.php?title=PMID:7050084&oldid=7142"