PMID:7050084

Citation	Böck, A, Turnowsky, F and Högenauer, G (1982) Tiamulin resistance mutations in Escherichia coli.J. Bacteriol. 151:1253-60
Abstract	Forty "two-step" and 13 "three-step" tiamulin-resistant mutants of Escherichia coli PR11 were isolated and tested for alteration of ribosomal proteins. Mutants with altered ribosomal proteins S10, S19, L3, and L4 were detected. The S19, L3, and L4 mutants were studied in detail. The L3 and L4 mutations did not segregate from the resistance character in transductional crosses and therefore seem to be responsible for the resistance. Extracts of these mutants also exhibited an increased in vitro resistance to tiamulin in the polyuridylic acid and phage R17 RNA-dependent polypeptide synthesis systems, and it was demonstrated that this was a property of the 50S subunit. In the case of the S19 mutant, genetic analysis showed segregation between resistance and the S19 alteration and therefore indicated that mutation of a protein other than S19 was responsible for the resistance phenotype. The isolated ribosomes of the S19, L3, and L4 mutants bound radioactive tiamulin with a considerably reduced strength when compared with those of wild-type cells. The association constants were lower by factors ranging from approximately 20 to 200. When heated in the presence of ammonium chloride, these ribosomes partially regained their avidity for tiamulin.
Links	PubMed
Keywords	Bacterial Proteins; Diterpenes; Drug Resistance, Microbial; Escherichia coli; Genes, Bacterial; Mutation; Ribosomal Proteins; Ribosomes; Transduction, Genetic
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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: TS14-13-10 NCBI Taxon ID: 83333	Genotype of Reference Strain: rpsS Genotype of Experimental Strain : rpsS	Reference Condition:	OMP:0000276	increased resistance to antibiotics	ECO:0000182	in vitro culture assay data	Three step tiamulin resistance, table 1 and 2
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: TS15-23 NCBI Taxon ID: 83333	Genotype of Reference Strain: rplD Genotype of Experimental Strain : rplD	Reference Condition:	OMP:0000276	increased resistance to antibiotics	ECO:0000182	in vitro culture assay data	Two step Tiamulin resistant, table 1 and 2
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: TS10-21 NCBI Taxon ID: 83333	Genotype of Reference Strain: rplC Genotype of Experimental Strain : rplC	Reference Condition:	OMP:0000276	increased resistance to antibiotics	ECO:0000182	in vitro culture assay data	rplC(L3) two step tiamulin resistant in strain 1 and 2
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