Difference between revisions of "PMID:385587"

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'''Fayerman, JT, Vann, MC, Williams, LS and Umbarger, HE'''  (1979) ilvU, a locus in Escherichia coli affecting the derepression of isoleucyl-tRNA synthetase and the RPC-5 chromatographic profiles of tRNAIle and tRNAVal.''J. Biol. Chem.'' '''254''':9429-40
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!align=left  |Abstract
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A mutation in the ilvU locus of Escherichia coli has led to a complex phenotype that included resistance to thiaisoleucine, a loss of derepressibility of isoleucyl tRNA synthetase, and an alteration of the RPC-5 chromatographic profile of the branched-chain aminoacyl-tRNA's. The alterations were manifest in an increase in the amount of Species 2 of both tRNAIle and tRNAVal at the expense of Species 1. A similar alteration, but independent of (and additive to) that caused by the ilvU mutation, was observed upon limitation of either isoleucine or valine. The shift in profile caused by limitation was also independent of the reduced growth rate or the derepression of the isoleucine and valine biosynthetic enzymes that also result from limitation. During chloramphenicol treatment nearly all tRNAIle and tRNAVal formed appears as species 2. Upon recovery from chloramphenicol, Species 2 of both acceptors are converted to Species 1. It is proposed that the ilvU product not only allows derepression of isoleucyl-tRNA synthetase but also retards the conversion of tRNA2Ile to tRNA1Ile and that of tRNA2Val to tRNA1Val. The mutated ilvU loci abolish the derepression and are more efficient in retarding the conversion.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=385587 PubMed]
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!align=left  |Keywords
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Amino Acyl-tRNA Synthetases; Chloramphenicol; Chromatography, High Pressure Liquid; Enzyme Repression; Escherichia coli; Genotype; Isoleucine; Isoleucine-tRNA Ligase; Mutation; Phenotype; RNA, Transfer; Valine
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==Main Points of the Paper ==
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{{LitSignificance}}
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== Materials and Methods Used ==
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{{LitMaterials}}
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==Phenotype Annotations==
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{{AnnotationTableHelp}}
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{|  id="A4fad87acabfff"  class=" tableEdit Phenotype_Table_2" 
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: CU426
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''ilvU''
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*Genotype of Experimental Strain : ''ilvU459''
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*Reference Condition:
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ECO:0000034
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non-traceable author statement
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Tiaisoleucine Resistant
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain:
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''ilvU''
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*Genotype of Experimental Strain : ''ilvU''
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*Reference Condition:
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derepressibility of isoleucyl tRNA synthetase
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: CU210
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''proA''
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*Genotype of Experimental Strain : ''proA''
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*Reference Condition:
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Nitro-4-pyridine N-oxide Resistance
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: CU210
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''proB''
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*Genotype of Experimental Strain : ''proB''
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*Reference Condition:
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Nitro-4-pyridine N-oxide Resistance
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==Notes==
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==References==
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{{RefHelp}}
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<references/>
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[[Category:Publication]] [[Category:Papers referenced in the LaRossa chapter]] [[Category:LaRossa citations that need review]]

Latest revision as of 17:09, 11 May 2012

Citation

Fayerman, JT, Vann, MC, Williams, LS and Umbarger, HE (1979) ilvU, a locus in Escherichia coli affecting the derepression of isoleucyl-tRNA synthetase and the RPC-5 chromatographic profiles of tRNAIle and tRNAVal.J. Biol. Chem. 254:9429-40

Abstract

A mutation in the ilvU locus of Escherichia coli has led to a complex phenotype that included resistance to thiaisoleucine, a loss of derepressibility of isoleucyl tRNA synthetase, and an alteration of the RPC-5 chromatographic profile of the branched-chain aminoacyl-tRNA's. The alterations were manifest in an increase in the amount of Species 2 of both tRNAIle and tRNAVal at the expense of Species 1. A similar alteration, but independent of (and additive to) that caused by the ilvU mutation, was observed upon limitation of either isoleucine or valine. The shift in profile caused by limitation was also independent of the reduced growth rate or the derepression of the isoleucine and valine biosynthetic enzymes that also result from limitation. During chloramphenicol treatment nearly all tRNAIle and tRNAVal formed appears as species 2. Upon recovery from chloramphenicol, Species 2 of both acceptors are converted to Species 1. It is proposed that the ilvU product not only allows derepression of isoleucyl-tRNA synthetase but also retards the conversion of tRNA2Ile to tRNA1Ile and that of tRNA2Val to tRNA1Val. The mutated ilvU loci abolish the derepression and are more efficient in retarding the conversion.

Links

PubMed

Keywords

Amino Acyl-tRNA Synthetases; Chloramphenicol; Chromatography, High Pressure Liquid; Enzyme Repression; Escherichia coli; Genotype; Isoleucine; Isoleucine-tRNA Ligase; Mutation; Phenotype; RNA, Transfer; Valine

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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: CU426
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ilvU
  • Genotype of Experimental Strain : ilvU459
  • Reference Condition:

ECO:0000034

non-traceable author statement

Tiaisoleucine Resistant

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ilvU
  • Genotype of Experimental Strain : ilvU
  • Reference Condition:

derepressibility of isoleucyl tRNA synthetase

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: CU210
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: proA
  • Genotype of Experimental Strain : proA
  • Reference Condition:

Nitro-4-pyridine N-oxide Resistance

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: CU210
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: proB
  • Genotype of Experimental Strain : proB
  • Reference Condition:

Nitro-4-pyridine N-oxide Resistance


edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

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