PMID:201607

Citation	Iwaya, M, Jones, CW, Khorana, J and Strominger, JL (1978) Mapping of the mecillinam-resistant, round morphological mutants of Escherichia coli.J. Bacteriol. 133:196-202
Abstract	Genes responsible for round morphology in mecillinam-resistant, round morphological mutants of Escherichia coli have been mapped. Three mutants, called rodX, mapped at around 14 min, and two, called rodY, mapped at around 70 min by P1 transduction. These are either the same or very close to the loci reported, respectively, for the rodA (H. Matsuzawa, K. Hayakawa, T. Sato, and K. Imahori, J. Bacteriol. 115:436-442, 1973) and envB genes (B. Westling-Häggström and S. Normark, J. Bacteriol. 123:75-82, 1975). This suggests that mecillinam can be used very efficiently to select for found morphological mutants of rodA and envB after nitrosoguanidine treatment.
Links	PubMed
Keywords	Amdinocillin; Chromosome Mapping; Escherichia coli; Genes; Mutation; Penicillanic Acid; Penicillin Resistance; Phenotype; Transduction, Genetic
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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Eshcerichia coli Strain: K-12 Substrain: HL18 NCBI Taxon ID: 83333	Genotype of Reference Strain: rodY Genotype of Experimental Strain : rodY -	Reference Condition:	OMP:0000276	increased resistance to antibiotics			Resistance to mecillinam
a mutation or genetic difference within a strain	Taxon: Eshcerichia coli Strain: K-12 Substrain: HL18 NCBI Taxon ID: 83333	Genotype of Reference Strain: rodY Genotype of Experimental Strain : rodY -	Reference Condition:					Round morphology
a mutation or genetic difference within a strain	Taxon: Eshcerichia coli Strain: K-12 Substrain: H3, HL29, HL103 NCBI Taxon ID: 83333	Genotype of Reference Strain: rodX Genotype of Experimental Strain : rodX -	Reference Condition:					Round morphology
a mutation or genetic difference within a strain	Taxon: Eshcerichia coli Strain: K-12 Substrain: H3, HL29, HL103 NCBI Taxon ID: 83333	Genotype of Reference Strain: rodX Genotype of Experimental Strain : rodX -	Reference Condition:	OMP:0000276	increased resistance to antibiotics			Resistance to mecillinam
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