Difference between revisions of "PMID:3309133"

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{|  id="O4fe894d927760"  class=" tableEdit PMID_info_table" 
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'''Pugsley, AP, Moreno, F and de Lorenzo, V'''  (1986) Microcin-E492-insensitive mutants of Escherichia coli K12.''J. Gen. Microbiol.'' '''132''':3253-9
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!align=left  |Abstract
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Mutations in three Escherichia coli K12 genes, tonB, exbB and the newly discovered semA, reduce sensitivity to the low Mr polypeptide antibiotic microcin E492. The products of the tonB and exbB genes were previously shown to be involved in the uptake of siderophore-complexed iron and in the action of a number of colicins. Strains mutated at or close to semA (collectively referred to as sem mutations) remained fully sensitive to these colicins, and grew as well as wild-type strains under conditions of iron starvation. Expression of a number of sem-lacZ operon fusions was not affected by iron limitation, and sem mutations did not affect the production of iron-regulated outer membrane proteins which are known or thought to be involved in iron uptake. Hfr conjugation and P1 phage transduction experiments indicated that semA is located close to pabB at 40 min on the E. coli K12 chromosome. This places semA close to the mng locus, wherein mutations result in decreased manganese sensitivity. However, strains carrying the semA mutation exhibited increased manganese sensitivity.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3309133 PubMed]
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!align=left  |Keywords
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Anti-Bacterial Agents; Bacteriocins; Chromosome Mapping; Cloning, Molecular; Drug Resistance, Microbial; Escherichia coli; Genes, Bacterial; Manganese; Mutation; Transduction, Genetic
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Revision as of 11:42, 25 June 2012

Citation

Pugsley, AP, Moreno, F and de Lorenzo, V (1986) Microcin-E492-insensitive mutants of Escherichia coli K12.J. Gen. Microbiol. 132:3253-9

Abstract

Mutations in three Escherichia coli K12 genes, tonB, exbB and the newly discovered semA, reduce sensitivity to the low Mr polypeptide antibiotic microcin E492. The products of the tonB and exbB genes were previously shown to be involved in the uptake of siderophore-complexed iron and in the action of a number of colicins. Strains mutated at or close to semA (collectively referred to as sem mutations) remained fully sensitive to these colicins, and grew as well as wild-type strains under conditions of iron starvation. Expression of a number of sem-lacZ operon fusions was not affected by iron limitation, and sem mutations did not affect the production of iron-regulated outer membrane proteins which are known or thought to be involved in iron uptake. Hfr conjugation and P1 phage transduction experiments indicated that semA is located close to pabB at 40 min on the E. coli K12 chromosome. This places semA close to the mng locus, wherein mutations result in decreased manganese sensitivity. However, strains carrying the semA mutation exhibited increased manganese sensitivity.

Links

PubMed

Keywords

Anti-Bacterial Agents; Bacteriocins; Chromosome Mapping; Cloning, Molecular; Drug Resistance, Microbial; Escherichia coli; Genes, Bacterial; Manganese; Mutation; Transduction, Genetic

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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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</protect>

Notes

References

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