PMID:2285321

Citation	Juhl, MJ and Clark, DP (1990) Thiophene-degrading Escherichia coli mutants possess sulfone oxidase activity and show altered resistance to sulfur-containing antibiotics.Appl. Environ. Microbiol. 56:3179-85
Abstract	We have previously isolated mutants of Escherichia coli which show increased oxidation of heterocyclic furan and thiophene substrates. We have now found that strains carrying the thdA mutation express a novel enzyme activity which oxidizes a variety of substrates containing a sulfone (SO2) moiety. Both heterocyclic sulfones (e.g., tetramethylene sulfone) and simple aliphatic sulfones (e.g., ethyl sulfone) were oxidized. The thdA mutants were more resistant than wild-type strains to aromatic sulfone antibiotics such as dapsone. In contrast they showed increased susceptibility to thiolutin, a cyclic antibiotic containing sulfur at the sulfide level of oxidation. Several new thdA mutant alleles were isolated by selecting for increased oxidation of various aliphatic sulfur compounds. These new thdA mutants showed similar sulfone oxidase activity and the same map location (at 10.7 min) as the original thdA1 mutation. The constitutive fadR mutation was required for the phenotypic expression of thdA-mediated oxidation of sulfur compounds. However, the thdA-directed expression of sulfone oxidase activity was not fadR dependent. The thdC and thdD mutations probably protect against the toxicity of thiophene derivatives rather than conferring improved metabolic capability.
Links	PubMed
Keywords	Anti-Bacterial Agents; Bacterial Proteins; Dapsone; Drug Resistance, Microbial; Escherichia coli; Furans; Genes, Bacterial; Oxidation-Reduction; Oxidoreductases; Oxidoreductases Acting on Sulfur Group Donors; Pyrrolidinones; Substrate Specificity; Sulfones; Sulfoxides; Thiazoles; Thiophenes
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: DC272 NCBI Taxon ID: 83333	Genotype of Reference Strain: thdA + Genotype of Experimental Strain : thdA2	Reference Condition:			0000033	traceable author statement	The thdA mutation caused hypersensitivity toward thiolutin on minimum medium. This includes mutants thdA3 as well as strains NAR11 & NAR41.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: DC272 NCBI Taxon ID: 83333	Genotype of Reference Strain: thdA + Genotype of Experimental Strain : thdA1	Reference Condition:			0000033	traceable author statement	The thdA mutation caused resistance to Dapsone. This was also observed in the mutants thdA2, thdA3 & thdA4.
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