Difference between revisions of "PMID:5119202"

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'''Goldschmidt, MC , Lockhart, BM  and Perry, K '''  (1971) Rapid methods for determining decarboxylase activity: ornithine and lysine decarboxylases. ''Appl Microbiol'' '''22''':344-9
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!align=left  |Abstract
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A rapid biochemical method for the determination of ornithine and lysine decarboxylase (EC 4.1.1.18) activity has been developed for use in the routine clinical laboratory. It is based on the detection of the amine end product produced in response to the single key amino acid added to a synthetic medium. A modified ninhydrin reagent is used to detect the amine after a chloroform extraction. This procedure can be used with a 1- to 4-hr incubation period (utilizing an initial concentrated inoculum) or with an overnight culture. Thus, measurements based on the alkalinization of the medium after a lengthy incubation period are avoided. Optimal parameters for enzyme activity are discussed.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=5119202 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC376312 PMC376312]
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Amines/biosynthesis; Bacteria/classification; Bacteria/enzymology; Bacteria/metabolism; Carboxy-Lyases/metabolism; Chloroform; Chromatography, Thin Layer; Culture Media; Enterobacteriaceae/classification; Enterobacteriaceae/enzymology; Enterobacteriaceae/metabolism; Evaluation Studies as Topic; Hydrogen-Ion Concentration; Indicators and Reagents; Lysine/metabolism; Methods; Ornithine/metabolism; Temperature
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Latest revision as of 17:18, 14 September 2012

Citation

Goldschmidt, MC , Lockhart, BM and Perry, K (1971) Rapid methods for determining decarboxylase activity: ornithine and lysine decarboxylases. Appl Microbiol 22:344-9

Abstract

A rapid biochemical method for the determination of ornithine and lysine decarboxylase (EC 4.1.1.18) activity has been developed for use in the routine clinical laboratory. It is based on the detection of the amine end product produced in response to the single key amino acid added to a synthetic medium. A modified ninhydrin reagent is used to detect the amine after a chloroform extraction. This procedure can be used with a 1- to 4-hr incubation period (utilizing an initial concentrated inoculum) or with an overnight culture. Thus, measurements based on the alkalinization of the medium after a lengthy incubation period are avoided. Optimal parameters for enzyme activity are discussed.

Links

PubMed PMC376312

Keywords

Amines/biosynthesis; Bacteria/classification; Bacteria/enzymology; Bacteria/metabolism; Carboxy-Lyases/metabolism; Chloroform; Chromatography, Thin Layer; Culture Media; Enterobacteriaceae/classification; Enterobacteriaceae/enzymology; Enterobacteriaceae/metabolism; Evaluation Studies as Topic; Hydrogen-Ion Concentration; Indicators and Reagents; Lysine/metabolism; Methods; Ornithine/metabolism; Temperature

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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

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<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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Notes

References

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