Difference between revisions of "PMID:20946848"

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'''De, N , Navarro, MV , Wang, Q , Krasteva, PV  and Sondermann, H '''  (2010) Biophysical assays for protein interactions in the Wsp sensory system and biofilm formation. ''Meth. Enzymol.'' '''471''':161-84
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!align=left  |Abstract
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Many signal transduction and regulatory events are mediated by a change in oligomeric state upon posttranslational modification or ligand binding. Hence, the characterization of proteins and protein complexes with respect to their size and shape is crucial for elucidating the molecular mechanisms that control their activities. Commonly used methods for the determination of molecular weights of biological polymers such as standard size-exclusion chromatography or analytical ultracentrifugation have been applied successfully but have some limitations. Static multiangle light scattering presents an attractive alternative approach for absolute molecular weight measurements in solution. We review the biophysical principles, advantages, and pitfalls of some popular methods for determining the quaternary structure of proteins, using the response regulator diguanylate cyclase WspR from Pseudomonas and FimX, a protein involved in Pseudomonas aeruginosa twitching motility, as examples.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=20946848 PubMed]
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Online version:[http://dx.doi.org/10.1016/S0076-6879(10)71010-7 10.1016/S0076-6879(10)71010-7]
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!align=left  |Keywords
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Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biofilms/growth & development; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Multiprotein Complexes/genetics; Multiprotein Complexes/metabolism; Phosphorus-Oxygen Lyases/genetics; Phosphorus-Oxygen Lyases/metabolism; Protein Binding/genetics; Protein Binding/physiology; Pseudomonas aeruginosa/genetics; Pseudomonas aeruginosa/metabolism; Signal Transduction/genetics; Signal Transduction/physiology
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Revision as of 19:18, 16 September 2012

Citation

De, N , Navarro, MV , Wang, Q , Krasteva, PV and Sondermann, H (2010) Biophysical assays for protein interactions in the Wsp sensory system and biofilm formation. Meth. Enzymol. 471:161-84

Abstract

Many signal transduction and regulatory events are mediated by a change in oligomeric state upon posttranslational modification or ligand binding. Hence, the characterization of proteins and protein complexes with respect to their size and shape is crucial for elucidating the molecular mechanisms that control their activities. Commonly used methods for the determination of molecular weights of biological polymers such as standard size-exclusion chromatography or analytical ultracentrifugation have been applied successfully but have some limitations. Static multiangle light scattering presents an attractive alternative approach for absolute molecular weight measurements in solution. We review the biophysical principles, advantages, and pitfalls of some popular methods for determining the quaternary structure of proteins, using the response regulator diguanylate cyclase WspR from Pseudomonas and FimX, a protein involved in Pseudomonas aeruginosa twitching motility, as examples.

Links

PubMed Online version:10.1016/S0076-6879(10)71010-7

Keywords

Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biofilms/growth & development; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Multiprotein Complexes/genetics; Multiprotein Complexes/metabolism; Phosphorus-Oxygen Lyases/genetics; Phosphorus-Oxygen Lyases/metabolism; Protein Binding/genetics; Protein Binding/physiology; Pseudomonas aeruginosa/genetics; Pseudomonas aeruginosa/metabolism; Signal Transduction/genetics; Signal Transduction/physiology

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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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Notes

References

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