Difference between revisions of "PMID:4935495"

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== Materials and Methods Used ==
 
== Materials and Methods Used ==
 
{{LitMaterials}}
 
{{LitMaterials}}
 +
 +
*IMViC tests
 +
**indole
 +
**methyl red
 +
**Vogues-Proskauer
 +
**citrate
 +
 +
*Isolation and Differentiation media
 +
**MacKonkey
 +
**deoxycholate
 +
**endo agar
 +
 +
*Media and tests selectively used for the identification of pink colonies
 +
**Trypticase for indole-check
 +
**10-µg ampicillin disc for Kirby-Bauer sensitivity testing on Mueller-Hinton Agar
 +
**motility-ornithine medium
 +
**lysine-iron agar
 +
**Koser citrate medium
 +
**Dimethyl-p-phenylenediamine-hydrochloride for the Kovacs oxidase test
 +
 +
*Nonmucoid strains
 +
*Mucoid strains
  
 
==Phenotype Annotations==
 
==Phenotype Annotations==

Latest revision as of 16:33, 18 September 2012

Citation

von Graevenitz, A (1971) Practical substitution for the indol, methyl red, Voges-Proskauer, citrate system. Appl Microbiol 21:1107-9

Abstract

A substitution for the indole, methyl red, Voges-Proskauer, citrate system is proposed. The scheme employs indole and ampicillin susceptibility tests routinely and uses selectively three other media for six reactions.

Links

PubMed PMC377358

Keywords

Ampicillin/pharmacology; Azo Compounds/metabolism; Bacteriological Techniques; Butanones/biosynthesis; Citrates/metabolism; Enterobacteriaceae/classification; Enterobacteriaceae/drug effects; Enterobacteriaceae/metabolism; Indoles/biosynthesis; Methods; Microbial Sensitivity Tests; Penicillin Resistance

Main Points of the Paper

Please summarize the main points of the paper.

  • A new system for identification is discussed
  • Substitutions for current clinical tests must be made because they:
    • are expensive
    • are time-consuming
    • may lead to misidentification

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

  • IMViC tests
    • indole
    • methyl red
    • Vogues-Proskauer
    • citrate
  • Isolation and Differentiation media
    • MacKonkey
    • deoxycholate
    • endo agar
  • Media and tests selectively used for the identification of pink colonies
    • Trypticase for indole-check
    • 10-µg ampicillin disc for Kirby-Bauer sensitivity testing on Mueller-Hinton Agar
    • motility-ornithine medium
    • lysine-iron agar
    • Koser citrate medium
    • Dimethyl-p-phenylenediamine-hydrochloride for the Kovacs oxidase test
  • Nonmucoid strains
  • Mucoid strains

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

</protect>

Notes

References

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