Difference between revisions of "PMID:5324399"

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==Main Points of the Paper ==
 
==Main Points of the Paper ==
 
{{LitSignificance}}
 
{{LitSignificance}}
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*Provides evidence that component M is a protein, distinct from β-galactosidase and thiogalactoside transacetylase
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*M is controlled by the i gene
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*M is localized to the membrane-containing particulate fraction and has a high affinity for certain β-galactosides
  
 
== Materials and Methods Used ==
 
== Materials and Methods Used ==
 
{{LitMaterials}}
 
{{LitMaterials}}
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 +
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*Strain: ML30 (i+z+y+x+)
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*Strain: ML308 (i-z+y+x+)
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*ONPG hydrolysis
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*thiogalactoside transacetylase assay
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*radio-labeled substrate protein binding assay
  
 
==Phenotype Annotations==
 
==Phenotype Annotations==

Latest revision as of 16:37, 18 September 2012

Citation

Fox, CF and Kennedy, EP (1965) Specific labeling and partial purification of the M protein, a component of the beta-galactoside transport system of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 54:891-9

Abstract

No abstract in PubMed

Links

PubMed PMC219761

Keywords

Bacterial Proteins; Biological Transport; Carbon Isotopes; Escherichia coli/enzymology; Ethylmaleimide/pharmacology; Galactosidases; Glycosides/metabolism

Main Points of the Paper

Please summarize the main points of the paper.

  • Provides evidence that component M is a protein, distinct from β-galactosidase and thiogalactoside transacetylase
  • M is controlled by the i gene
  • M is localized to the membrane-containing particulate fraction and has a high affinity for certain β-galactosides

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).


  • Strain: ML30 (i+z+y+x+)
  • Strain: ML308 (i-z+y+x+)
  • ONPG hydrolysis
  • thiogalactoside transacetylase assay
  • radio-labeled substrate protein binding assay

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

</protect>

Notes

References

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