PMID:3881393

Citation	Sullivan, MA, Cannon, JF, Webb, FH and Bock, RM (1985) Antisuppressor mutation in Escherichia coli defective in biosynthesis of 5-methylaminomethyl-2-thiouridine.J. Bacteriol. 161:368-76
Abstract	Mutations in three Escherichia coli K-12 genes were isolated that reduce the efficiency of the lysine-inserting nonsense suppressor supL. These antisuppressor mutations asuD, asuE, and asuF map at 61.9, 25.3, and 76.3 min, respectively, on the E. coli chromosome. Biochemical and genetic analysis of the mutant strains revealed the reason for the antisuppressor phenotype for two of these genes. The activity of lysyl-tRNA synthetase was reduced in strains with asuD mutations. The modification of 5-methylaminomethyl-2-thiouridine, the wobble base of tRNALys, was impaired in asuE mutant strains, presumably at the 2-thiolation step.
Links	PubMed
Keywords	Chromosome Mapping; Chromosomes, Bacterial; Escherichia coli; Lysine-tRNA Ligase; Plasmids; RNA, Transfer, Amino Acyl; Suppression, Genetic; Thiouridine; Transduction, Genetic
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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: MK60 NCBI Taxon ID: 83333	Genotype of Reference Strain: asu+ lysT(supL) Genotype of Experimental Strain : asuD6 lysT(supL)	Reference Condition:					supL suppressor gene is deactivated buy asuD mutation see figure 3 for full experimental results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: MK60 NCBI Taxon ID: 83333	Genotype of Reference Strain: asu+ lysT(supL) Genotype of Experimental Strain : asuE9 lysT(supL)	Reference Condition:					supL suppressor gene is deactivated buy asuE mutation see figure 3 for full experimental results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: MK60 NCBI Taxon ID: 83333	Genotype of Reference Strain: asu+ lysT(supL) Genotype of Experimental Strain : asuF12 lysT(supL)	Reference Condition:					supL suppressor gene is deactivated buy asuF mutation see figure 3 for full experimental results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: MK60 NCBI Taxon ID: 83333	Genotype of Reference Strain: asu+ glnU(supB) Genotype of Experimental Strain : asuE9 glnU(supB)	Reference Condition:					Suppressor glnU is deactivated by the asuE mutation. See figure 3 for full experimental results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: MK60 NCBI Taxon ID: 83333	Genotype of Reference Strain: asu+ glnU(supB) Genotype of Experimental Strain : asuF12 glnU(supB)	Reference Condition:					Suppressor glnU is deactivated by the asuF mutation. See figure 3 for full experimental results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: MK60 NCBI Taxon ID: 83333	Genotype of Reference Strain: asu+ tyrT Genotype of Experimental Strain : asuE9 tyrT(supC)	Reference Condition:					Suppressor gene tyrT(supC) is deactivated by asuE mutation. See figure 3 for full experimental results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: MK60 NCBI Taxon ID: 83333	Genotype of Reference Strain: asu+ tyrT Genotype of Experimental Strain : asuF12 tyrT(supC)	Reference Condition:					Suppressor gene tyrT(supC) is deactivated by asuF mutation. See figure 3 for full experimental results.
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Notes

• Through P1 transduction experiments asuD was found mapped at 61.9 min, asuE at 25.3 min and asuF at 76.3 min.

References

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