Difference between revisions of "PMID:794878"

From omp dev
Jump to: navigation, search
(Fill PMID: Page!)
(Table edited by KSharma via TableEdit)
 
(6 intermediate revisions by 3 users not shown)
Line 14: Line 14:
 
||
 
||
 
'''Gellert, M, O'Dea, MH, Itoh, T and Tomizawa, J'''  (1976) Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase.''Proc. Natl. Acad. Sci. U.S.A.'' '''73''':4474-8
 
'''Gellert, M, O'Dea, MH, Itoh, T and Tomizawa, J'''  (1976) Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase.''Proc. Natl. Acad. Sci. U.S.A.'' '''73''':4474-8
 +
||
 +
 
|-
 
|-
 
!align=left  |Abstract
 
!align=left  |Abstract
 
||
 
||
 
Novobiocin and coumermycin are known to inhibit the replication of DNA iing of DNA catalyzed by E. coli DNA gyrase, a recently discovered enzyme that introduces negative superhelical turns into covalently circular DNA. The activity of DNA gyrase purified from a coumermycin-resistant mutant strain is resistant to both drugs. The inhibition by novobiocin of colicin E1 plasmid DNA replication in a cell-free system is partially relieved by adding resistant DNA gyrase. Both in the case of coliclls. DNA molecules which are converted to the covalently circular form in thepresence of coumermycin remain relaxed, instead of achieving their normal supercoiled conformation. We conclude that DNA gyrase controls the supercoiling of DNA in E. coli.
 
Novobiocin and coumermycin are known to inhibit the replication of DNA iing of DNA catalyzed by E. coli DNA gyrase, a recently discovered enzyme that introduces negative superhelical turns into covalently circular DNA. The activity of DNA gyrase purified from a coumermycin-resistant mutant strain is resistant to both drugs. The inhibition by novobiocin of colicin E1 plasmid DNA replication in a cell-free system is partially relieved by adding resistant DNA gyrase. Both in the case of coliclls. DNA molecules which are converted to the covalently circular form in thepresence of coumermycin remain relaxed, instead of achieving their normal supercoiled conformation. We conclude that DNA gyrase controls the supercoiling of DNA in E. coli.
 +
||
 +
 
|-
 
|-
 
!align=left  |Links
 
!align=left  |Links
 
||
 
||
 
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=794878 PubMed]
 
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=794878 PubMed]
 +
 +
||
  
 
|-
 
|-
Line 27: Line 33:
 
||
 
||
 
Anti-Bacterial Agents; Colicins; Coumarins; DNA Replication; DNA, Bacterial; DNA, Circular; DNA, Viral; Escherichia coli; Mutation; Novobiocin; Nucleic Acid Conformation; Plasmids; Pyrroles
 
Anti-Bacterial Agents; Colicins; Coumarins; DNA Replication; DNA, Bacterial; DNA, Circular; DNA, Viral; Escherichia coli; Mutation; Novobiocin; Nucleic Acid Conformation; Plasmids; Pyrroles
 +
||
 +
  
 
|- class="tableEdit_footer"  
 
|- class="tableEdit_footer"  
−
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3291.T504cb59ecfa64&page=3291&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> ||
+
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3291.T504cb59ecfa64&page=3291&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> || ||
 
|}
 
|}
 
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.3291.T504cb59ecfa64-->
 
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.3291.T504cb59ecfa64-->
Line 51: Line 59:
 
|-
 
|-
 
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
 
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
|
 +
*Genotype of Reference Strain:
 +
|
 +
*Reference Condition:
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: Col E1
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''gyrB<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''gyrB'' mutation
 +
|
 +
*Reference Condition:
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
 +
As seen in figure 1 (lanes i-n) cou<sup>r</sup> was used to confer resistance toward Novobiocin.
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: E-coli
 +
*Strain: K-12
 +
*Substrain: Coli E1
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''gyrB<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''gyrB'' mutation
 +
|
 +
*Reference Condition: with novobiocin
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
 +
Refer to fig 1 lanes (o-p), gel electrophoresis.
 +
Two enzymes, sensitive (N99 ''recB'') and resistant(NI748), were mixed together and found 50% inhibition of novobiocin.
 +
|
 +
  
 
|- class="tableEdit_footer"  
 
|- class="tableEdit_footer"  

Latest revision as of 19:03, 1 November 2012

Citation

Gellert, M, O'Dea, MH, Itoh, T and Tomizawa, J (1976) Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase.Proc. Natl. Acad. Sci. U.S.A. 73:4474-8

Abstract

Novobiocin and coumermycin are known to inhibit the replication of DNA iing of DNA catalyzed by E. coli DNA gyrase, a recently discovered enzyme that introduces negative superhelical turns into covalently circular DNA. The activity of DNA gyrase purified from a coumermycin-resistant mutant strain is resistant to both drugs. The inhibition by novobiocin of colicin E1 plasmid DNA replication in a cell-free system is partially relieved by adding resistant DNA gyrase. Both in the case of coliclls. DNA molecules which are converted to the covalently circular form in thepresence of coumermycin remain relaxed, instead of achieving their normal supercoiled conformation. We conclude that DNA gyrase controls the supercoiling of DNA in E. coli.

Links

PubMed

Keywords

Anti-Bacterial Agents; Colicins; Coumarins; DNA Replication; DNA, Bacterial; DNA, Circular; DNA, Viral; Escherichia coli; Mutation; Novobiocin; Nucleic Acid Conformation; Plasmids; Pyrroles


edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Genotype of Reference Strain:
  • Reference Condition:

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: Col E1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: gyrB+
  • Genotype of Experimental Strain : gyrB mutation
  • Reference Condition:

As seen in figure 1 (lanes i-n) cour was used to confer resistance toward Novobiocin.

a mutation or genetic difference within a strain

  • Taxon: E-coli
  • Strain: K-12
  • Substrain: Coli E1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: gyrB+
  • Genotype of Experimental Strain : gyrB mutation
  • Reference Condition: with novobiocin

Refer to fig 1 lanes (o-p), gel electrophoresis. Two enzymes, sensitive (N99 recB) and resistant(NI748), were mixed together and found 50% inhibition of novobiocin.


edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

Retrieved from "http://bcbp-ftq4xm2.biobio.tamu.edu/omp/dev/index.php?title=PMID:794878&oldid=8688"