Difference between revisions of "PMID:135755"
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metabolite accumalation | metabolite accumalation | ||
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| − | + | Transport assay evidence | |
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Mutation in the ''tonB'' gene caused decreased uptake in Vitamin B<sub>12</sub>. See Figure 1 for experiment results. | Mutation in the ''tonB'' gene caused decreased uptake in Vitamin B<sub>12</sub>. See Figure 1 for experiment results. | ||
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metabolite accumalation | metabolite accumalation | ||
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| − | + | 0000134 | |
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| − | + | Transport assay evidence | |
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Mutation in the ''tonB'' gene caused decreased uptake in Vitamin B<sub>12</sub>. See Figure 1 for experiment results. | Mutation in the ''tonB'' gene caused decreased uptake in Vitamin B<sub>12</sub>. See Figure 1 for experiment results. | ||
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metabolite accumalation | metabolite accumalation | ||
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| − | + | biochemical trait analysis evidence | |
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It was seen through the use of a growth assay that the ''tonB'' mutation caused a decrease in Vitamin B<sub>12</sub> uptake. This was also seen in mutant strain's RK4128, RK4129 and RK4130. See table 3 for full experimental results. | It was seen through the use of a growth assay that the ''tonB'' mutation caused a decrease in Vitamin B<sub>12</sub> uptake. This was also seen in mutant strain's RK4128, RK4129 and RK4130. See table 3 for full experimental results. | ||
Latest revision as of 18:14, 7 November 2012
| Citation |
Bassford, PJ Jr, Bradbeer, C , Kadner, RJ and Schnaitman, CA (1976) Transport of vitamin B12 in tonB mutants of Escherichia coli. J. Bacteriol. 128:242-7 |
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| Abstract |
It is known that the tonB mutation in Escherichia coli is responsible for a defect in the transport of iron chelates. These are transported by systems that involve outer membrane components. We found that tonB mutants were also deficient in the secondary, energy-dependent phase of vitamin B12 transport, although the mutants have normal levels of B12 receptors on their cell surface. In addition, tonB mutants derived from vitamin B12 auxotrophs required elevated levels of B12 for normal growth. Maltose uptake, mediated by another transport system involving an outer membrane component, was unaffected by the tonB mutation. |
| Links | |
| Keywords |
Amino Acids/metabolism; Biological Transport, Active; Enterobactin/metabolism; Escherichia coli/growth & development; Escherichia coli/metabolism; Genes; Maltose/metabolism; Mutation; Protein Biosynthesis; Receptors, Drug; Vitamin B 12/metabolism |
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Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
|
a mutation or genetic difference within a strain |
|
|
|
0000252 |
metabolite accumalation |
0000134 |
Transport assay evidence |
Mutation in the tonB gene caused decreased uptake in Vitamin B12. See Figure 1 for experiment results. |
|
|
a mutation or genetic difference within a strain |
|
|
|
0000252 |
metabolite accumalation |
0000134 |
Transport assay evidence |
Mutation in the tonB gene caused decreased uptake in Vitamin B12. See Figure 1 for experiment results. |
|
|
a mutation or genetic difference within a strain |
|
|
|
0000252 |
metabolite accumalation |
0000172 |
biochemical trait analysis evidence |
It was seen through the use of a growth assay that the tonB mutation caused a decrease in Vitamin B12 uptake. This was also seen in mutant strain's RK4128, RK4129 and RK4130. See table 3 for full experimental results. |
|
| edit table |
</protect>
Notes
References
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