PMID:6808961

Citation	Brunner, DP , Graham, GS and Treick, RW (1982) Exchange of colicin receptor capacity between strains of Escherichia coli sensitive or resistant to colicin K-K235. Arch. Microbiol. 131:229-34
Abstract	Phage and colicin-resistant mutants were derived from Escherichia coli K-12P678. Two classes of phage T6 and colicin K-resistant mutants (genotype tsx) were isolated. Tsx-2 mutants, which demonstrated mucoid growth and increased sensitivities to many antibiotics, became sensitive to colicin K when pretreated with ethylenediaminetetraacetate (EDTA), whereas Tsx-1 mutants did not. Reassociation of EDTA-released material partially restored resistance to colicin K for Tsx-2 mutants. When EDTA-released material from strain P678 was associated with either class of K-resistant mutant, an increase in colicin K sensitivity resulted. Observations suggest that colicin K can act on its target site once it penetrates the cell surface. In addition, results suggest that functional colicin K receptors can be transferred from sensitive to resistant strains, thus conferring colicin sensitivity.
Links	PubMed
Keywords	Cell Membrane/metabolism; Colicins/metabolism; Colicins/pharmacology; Coliphages/genetics; Drug Resistance, Microbial; Edetic Acid/pharmacology; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Kinetics; Membrane Proteins/isolation & purification; Receptors, Cell Surface; Receptors, Immunologic/genetics; Receptors, Immunologic/metabolism
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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: P674 NCBI Taxon ID: 83333	Genotype of Reference Strain: tsx+ Genotype of Experimental Strain : tsx-2 in strain KG16	Reference Condition:			0000181	in vitro assay evidence	The tsx-2 class mutants were resistant toward the Phage T6.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: P674 NCBI Taxon ID: 83333	Genotype of Reference Strain: tsx+ Genotype of Experimental Strain : tsx-2 in strain KG16	Reference Condition:			0000181	in vitro assay evidence	The tsx-2 class mutants were resistant toward the Colicin K.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: P674 NCBI Taxon ID: 83333	Genotype of Reference Strain: tsx+ Genotype of Experimental Strain : tsx-1 in GG24	Reference Condition:			0000181	in vitro assay evidence	The tsx-1 class of mutants contained a slight resistances toward phage T6
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: P674 NCBI Taxon ID: 83333	Genotype of Reference Strain: tsx+ Genotype of Experimental Strain : tsx-1 in GG24	Reference Condition:			0000181	in vitro assay evidence	The tsx-1 class of mutants contained a slight resistances toward Colicin K.
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References

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