PMID:350837

Citation	Duncan, BK , Rockstroh, PA and Warner, HR (1978) Escherichia coli K-12 mutants deficient in uracil-DNA glycosylase. J. Bacteriol. 134:1039-45
Abstract	A new assay specific for uracil-DNA glycosylase is described, Escherichia coli mutants partially and totally deficient in uracil-DNA glycosylase activity have been isolated by using this assay in mass-screening procedures. These have been designated ung mutants. The ung gene maps between tyrA and nadB on the E. coli chromosome. T4 phage containing uracil in their DNA grow on the most glycosylase-deficient hosts but are unable to grow on wild-type bacteria. This provides a simple spot test for the ung genotype. The ung mutants show slightly higher rates of spontaneous mutation to antibiotic resistance. Taken together, these results suggest a central role for uracil-DNA glycosylase in the initiation of an excision repair pathway for the exclusion of uracil from DNA.
Links	PubMed PMC222353
Keywords	Chromosome Mapping; Coliphages/analysis; Coliphages/growth & development; DNA Repair; DNA, Viral/analysis; Deoxyuracil Nucleotides; Escherichia coli/genetics; Genes; Mutation; N-Glycosyl Hydrolases/genetics; Uracil/analysis
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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: W3110 NCBI Taxon ID: 83333	Genotype of Reference Strain: ung+ Genotype of Experimental Strain : ung- in strain BD10	Reference Condition: 25o C Experimental Condition: 42oC	0000298	enzyme activity phenotype	0000178	in vivo assay evidence	ung mutation caused reduced Uracil-DNA glycosylase activity at 42 o C. Also seen in strain BD13. See table 2.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: W3110 NCBI Taxon ID: 83333	Genotype of Reference Strain: ung+ Genotype of Experimental Strain : ung-6- in strain BD1124	Reference Condition: 25o C Experimental Condition: 42oC	0000298	enzyme activity phenotype	0000178	in vivo assay evidence	ung mutation caused reduced Uracil-DNA glycosylase activity at 45 o C, roughly 95 percent compared to the wild type. See figure 1 for full results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: W3110 NCBI Taxon ID: 83333	Genotype of Reference Strain: ung+ Genotype of Experimental Strain : ung-6- in strain BD1124	Reference Condition: 25o C Experimental Condition: 42oC	0000298	enzyme activity phenotype	0000178	in vivo assay evidence	ung mutation caused reduced Uracil-DNA glycosylase activity at 45 o C, roughly 95 percent compared to the wild type. See figure 1 for full results.
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