Difference between revisions of "PMID:16621832"

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{|  id="T50d2856048e4e"  class=" tableEdit PMID_info_table" 
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!align=left  |Citation
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'''Ackerley, DF , Barak, Y , Lynch, SV , Curtin, J  and Matin, A '''  (2006) Effect of chromate stress on Escherichia coli K-12. ''J. Bacteriol.'' '''188''':3371-81
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!align=left  |Abstract
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The nature of the stress experienced by Escherichia coli K-12 exposed to chromate, and mechanisms that may enable cells to withstand this stress, were examined. Cells that had been preadapted by overnight growth in the presence of chromate were less stressed than nonadapted controls. Within 3 h of chromate exposure, the latter ceased growth and exhibited extreme filamentous morphology; by 5 h there was partial recovery with restoration of relatively normal cell morphology. In contrast, preadapted cells were less drastically affected in their morphology and growth. Cellular oxidative stress, as monitored by use of an H2O2-responsive fluorescent dye, was most severe in the nonadapted cells at 3 h postinoculation, lower in the partially recovered cells at 5 h postinoculation, and lower still in the preadapted cells. Chromate exposure depleted cellular levels of reduced glutathione and other free thiols to a greater extent in nonadapted than preadapted cells. In both cell types, the SOS response was activated, and levels of proteins such as SodB and CysK, which can counter oxidative stress, were increased. Some mutants missing antioxidant proteins (SodB, CysK, YieF, or KatE) were more sensitive to chromate. Thus, oxidative stress plays a major role in chromate toxicity in vivo, and cellular defense against this toxicity involves activation of antioxidant mechanisms. As bacterial chromate bioremediation is limited by the toxicity of chromate, minimizing oxidative stress during bacterial chromate reduction and bolstering the capacity of these organisms to deal with this stress will improve their effectiveness in chromate bioremediation.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16621832 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1447458 PMC1447458]
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Online version:[http://dx.doi.org/10.1128/JB.188.9.3371-3381.2006 10.1128/JB.188.9.3371-3381.2006]
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!align=left  |Keywords
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Adaptation, Physiological; Chromates; Culture Media; Escherichia coli K12/cytology; Escherichia coli K12/growth & development; Escherichia coli K12/physiology; Escherichia coli Proteins/metabolism; Oxidative Stress; beta-Galactosidase/metabolism
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==Main Points of the Paper ==
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{{LitSignificance}}
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== Materials and Methods Used ==
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{{LitMaterials}}
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==Phenotype Annotations==
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{{AnnotationTableHelp}}
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="F50d285608eb76"  class=" tableEdit Phenotype_Table_2" 
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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cysK
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*Taxon: Escherichia coli
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*Strain:  K-12
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*Substrain: BW25113
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*NCBI Taxon ID: 679895
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*Genotype of Reference Strain:
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*Reference Condition:
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WT Chromate MIC50 (?M) 400
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cysN
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: BW25113
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*NCBI Taxon ID: 679895
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|
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*Genotype of Reference Strain:
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*Reference Condition:
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WT Chromate MIC50 (?M) 400
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katE
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: BW25113
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*NCBI Taxon ID: 679895
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|
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*Genotype of Reference Strain:
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|
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*Reference Condition:
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katG
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: BW25113
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*NCBI Taxon ID: 679895
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|
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*Genotype of Reference Strain:
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*Reference Condition:
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gshA
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: BW25113
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*NCBI Taxon ID: 679895
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|
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*Genotype of Reference Strain:
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*Reference Condition:
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gshB
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: BW25113
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*NCBI Taxon ID: 679895
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*Genotype of Reference Strain:
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*Reference Condition:
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sodA
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: BW25113
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*NCBI Taxon ID: 679895
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*Genotype of Reference Strain:
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*Reference Condition:
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sodB
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: BW25113
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*NCBI Taxon ID: 679895
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|
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*Genotype of Reference Strain:
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*Reference Condition:
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yieF
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: BW25113
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*NCBI Taxon ID: 679895
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*Genotype of Reference Strain:
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*Reference Condition:
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fliC
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: BW25113
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*NCBI Taxon ID: 679895
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*Genotype of Reference Strain:
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*Reference Condition:
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ompW
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: BW25113
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*NCBI Taxon ID: 679895
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|
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*Genotype of Reference Strain:
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*Reference Condition:
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ftnA
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: BW25113
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*NCBI Taxon ID: 679895
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|
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*Genotype of Reference Strain:
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*Reference Condition:
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sspA
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: BW25113
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*NCBI Taxon ID: 679895
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*Genotype of Reference Strain:
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*Reference Condition:
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cysK
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*Taxon: Escherichia coli
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*Strain:  K-12
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*Substrain: BW25113
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*NCBI Taxon ID: 679895
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|
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*Genotype of Reference Strain:
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|
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*Reference Condition:
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|
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WT Chromate MIC50 (?M) 400
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|
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|- class="tableEdit_footer"
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|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3592.F50d285608eb76&page=3592&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</span> || || || || || || || || ||
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==Notes==
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==References==
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{{RefHelp}}
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<references/>
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[[Category:Publication]]

Latest revision as of 22:44, 19 December 2012

Citation

Ackerley, DF , Barak, Y , Lynch, SV , Curtin, J and Matin, A (2006) Effect of chromate stress on Escherichia coli K-12. J. Bacteriol. 188:3371-81

Abstract

The nature of the stress experienced by Escherichia coli K-12 exposed to chromate, and mechanisms that may enable cells to withstand this stress, were examined. Cells that had been preadapted by overnight growth in the presence of chromate were less stressed than nonadapted controls. Within 3 h of chromate exposure, the latter ceased growth and exhibited extreme filamentous morphology; by 5 h there was partial recovery with restoration of relatively normal cell morphology. In contrast, preadapted cells were less drastically affected in their morphology and growth. Cellular oxidative stress, as monitored by use of an H2O2-responsive fluorescent dye, was most severe in the nonadapted cells at 3 h postinoculation, lower in the partially recovered cells at 5 h postinoculation, and lower still in the preadapted cells. Chromate exposure depleted cellular levels of reduced glutathione and other free thiols to a greater extent in nonadapted than preadapted cells. In both cell types, the SOS response was activated, and levels of proteins such as SodB and CysK, which can counter oxidative stress, were increased. Some mutants missing antioxidant proteins (SodB, CysK, YieF, or KatE) were more sensitive to chromate. Thus, oxidative stress plays a major role in chromate toxicity in vivo, and cellular defense against this toxicity involves activation of antioxidant mechanisms. As bacterial chromate bioremediation is limited by the toxicity of chromate, minimizing oxidative stress during bacterial chromate reduction and bolstering the capacity of these organisms to deal with this stress will improve their effectiveness in chromate bioremediation.

Links

PubMed PMC1447458 Online version:10.1128/JB.188.9.3371-3381.2006

Keywords

Adaptation, Physiological; Chromates; Culture Media; Escherichia coli K12/cytology; Escherichia coli K12/growth & development; Escherichia coli K12/physiology; Escherichia coli Proteins/metabolism; Oxidative Stress; beta-Galactosidase/metabolism

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

cysK

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: BW25113
  • NCBI Taxon ID: 679895
  • Genotype of Reference Strain:
  • Reference Condition:

WT Chromate MIC50 (?M) 400

cysN

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: BW25113
  • NCBI Taxon ID: 679895
  • Genotype of Reference Strain:
  • Reference Condition:

WT Chromate MIC50 (?M) 400

katE

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: BW25113
  • NCBI Taxon ID: 679895
  • Genotype of Reference Strain:
  • Reference Condition:

katG

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: BW25113
  • NCBI Taxon ID: 679895
  • Genotype of Reference Strain:
  • Reference Condition:

gshA

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: BW25113
  • NCBI Taxon ID: 679895
  • Genotype of Reference Strain:
  • Reference Condition:

gshB

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: BW25113
  • NCBI Taxon ID: 679895
  • Genotype of Reference Strain:
  • Reference Condition:

sodA

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: BW25113
  • NCBI Taxon ID: 679895
  • Genotype of Reference Strain:
  • Reference Condition:

sodB

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: BW25113
  • NCBI Taxon ID: 679895
  • Genotype of Reference Strain:
  • Reference Condition:

yieF

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: BW25113
  • NCBI Taxon ID: 679895
  • Genotype of Reference Strain:
  • Reference Condition:

fliC

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: BW25113
  • NCBI Taxon ID: 679895
  • Genotype of Reference Strain:
  • Reference Condition:

ompW

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: BW25113
  • NCBI Taxon ID: 679895
  • Genotype of Reference Strain:
  • Reference Condition:

ftnA

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: BW25113
  • NCBI Taxon ID: 679895
  • Genotype of Reference Strain:
  • Reference Condition:

sspA

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: BW25113
  • NCBI Taxon ID: 679895
  • Genotype of Reference Strain:
  • Reference Condition:

cysK

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: BW25113
  • NCBI Taxon ID: 679895
  • Genotype of Reference Strain:
  • Reference Condition:

WT Chromate MIC50 (?M) 400


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</protect>

Notes

References

See Help:References for how to manage references in omp dev.

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