Difference between revisions of "PMID:5327656"

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(Materials and Methods Used)
(Table edited by Azweifel via TableEdit)
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NCBI:562
 
NCBI:562
 
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&Delta;''lac su-'' /F''i<sup>s</sup>o+z+y+''
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&Delta;''lac su-'' /F''i<sup>S</sup>o<sup>+</sup>z<sup>+</sup>y<sup>+</sup>''
 
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''lacI<sup>S</sup>''
 +
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 +
unable to utilize lactose
 
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deficient in lactose metabolism (''lac-'')
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Expression
 
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Metabolic Activity
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dominant over ''i<sup>+</sup>'' and not inducible by lactose
 
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Biochemical Assay
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LacZ assay
 
  
 
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Revision as of 17:50, 16 December 2010

Citation

Bourgeois, S, Cohn, M and Orgel, LE (1965) Suppression of and complementation among mutants of the regulatory gene of the lactose operon of Escherichia coli.J. Mol. Biol. 14:300-2

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Escherichia coli; Genes; Genetic Code; Lactose; Molecular Biology; Mutation

Main Points of the Paper

  • The regulatory gene, i, codes for a protein whereas the operator gene, o, probably does not

Materials and Methods Used

  • Parent Strain: Δlac su-/ FiSo+z+y+
    • iS- dominant over i+ and not inducible by lactose sz 43
  • 5 strains with suppressor mutations
    • su-
    • su+1
    • su+2
    • su+3
    • su+4

Phenotype Annotations

<protect>

Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes

Escherichia coli

NCBI:562

Δlac su- /FiSo+z+y+

lacIS

unable to utilize lactose

Expression

dominant over i+ and not inducible by lactose


</protect>

Notes