Difference between revisions of "PMID:3050121"

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{|  id="G4fc510540251f"  class=" tableEdit PMID_info_table" 
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'''Jin, DJ and Gross, CA'''  (1988) Mapping and sequencing of mutations in the Escherichia coli rpoB gene that lead to rifampicin resistance.''J. Mol. Biol.'' '''202''':45-58
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!align=left  |Abstract
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Rifampicin is an antibiotic that inhibits the function of RNA polymerase in eubacteria. Mutations affecting the beta subunit of RNA polymerase can confer resistance to rifampicin. A large number of rifampicin-resistant (hereafter called Rifr) mutants have been isolated in Escherichia coli to probe the involvement of RNA polymerase in a variety of physiological processes. We have undertaken a comprehensive analysis of Rifr mutations to identify their structural and functional effects on RNA polymerase. Forty-two Rifr isolates with a variety of phenotypes were mapped to defined intervals within the rpoB gene using a set of deletions of the rpoB gene. The mutations were sequenced. Seventeen mutational alterations affecting 14 amino acid residues were identified. These alleles are located in three distinct clusters in the center of the rpoB gene. We discuss the implications of our results with regards to the structure of the rifampicin binding site.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3050121 PubMed]
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!align=left  |Keywords
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Base Sequence; Chromosome Mapping; DNA-Directed RNA Polymerases; Drug Resistance, Microbial; Escherichia coli; Genes, Bacterial; Molecular Sequence Data; Mutation; Plasmids; Rifampin
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==Main Points of the Paper ==
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{{LitSignificance}}
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== Materials and Methods Used ==
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{{LitMaterials}}
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==Phenotype Annotations==
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="R4fc51054056c1"  class=" tableEdit Phenotype_Table_2" 
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain:
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''rpoB''
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*Genotype of Experimental Strain : ''rpoB 3445''
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*Reference Condition: 37C
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*Experimental Condition: 37C
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OMP:0000276
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increased resistance to antimicrobial compound
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ECO:0000178
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in vivo assay data
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Resistance to rifampicin at different concentrations in vivo
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain:
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''rpoB''
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*Genotype of Experimental Strain : ''rpoB 3445''
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*Reference Condition: 37C
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*Experimental Condition: 37C
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OMP:0000276
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increased resistance to antimicrobial compound
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ECO:0000178
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in vivo assay data
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Resistance to rifampicin at different concentrations  In vitro.
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==Notes==
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More specific terms requested already for OMP and ECO. OMP: rifampycin phenotype and ECO solid medium assay.
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==References==
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{{RefHelp}}
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<references/>
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[[Category:Publication]] [[Category:Papers referenced in the LaRossa chapter]] [[Category:LaRossa citations that need review]]

Latest revision as of 13:11, 24 January 2013

Citation

Jin, DJ and Gross, CA (1988) Mapping and sequencing of mutations in the Escherichia coli rpoB gene that lead to rifampicin resistance.J. Mol. Biol. 202:45-58

Abstract

Rifampicin is an antibiotic that inhibits the function of RNA polymerase in eubacteria. Mutations affecting the beta subunit of RNA polymerase can confer resistance to rifampicin. A large number of rifampicin-resistant (hereafter called Rifr) mutants have been isolated in Escherichia coli to probe the involvement of RNA polymerase in a variety of physiological processes. We have undertaken a comprehensive analysis of Rifr mutations to identify their structural and functional effects on RNA polymerase. Forty-two Rifr isolates with a variety of phenotypes were mapped to defined intervals within the rpoB gene using a set of deletions of the rpoB gene. The mutations were sequenced. Seventeen mutational alterations affecting 14 amino acid residues were identified. These alleles are located in three distinct clusters in the center of the rpoB gene. We discuss the implications of our results with regards to the structure of the rifampicin binding site.

Links

PubMed

Keywords

Base Sequence; Chromosome Mapping; DNA-Directed RNA Polymerases; Drug Resistance, Microbial; Escherichia coli; Genes, Bacterial; Molecular Sequence Data; Mutation; Plasmids; Rifampin

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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: rpoB
  • Genotype of Experimental Strain : rpoB 3445
  • Reference Condition: 37C
  • Experimental Condition: 37C

OMP:0000276

increased resistance to antimicrobial compound

ECO:0000178

in vivo assay data

Resistance to rifampicin at different concentrations in vivo

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: rpoB
  • Genotype of Experimental Strain : rpoB 3445
  • Reference Condition: 37C
  • Experimental Condition: 37C

OMP:0000276

increased resistance to antimicrobial compound

ECO:0000178

in vivo assay data

Resistance to rifampicin at different concentrations In vitro.


edit table

</protect>

Notes

More specific terms requested already for OMP and ECO. OMP: rifampycin phenotype and ECO solid medium assay.

References

See Help:References for how to manage references in omp dev.

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