Difference between revisions of "PMID:8168498"

From omp dev
Jump to: navigation, search
(Fill PMID: Page!)
(→‎References)
 
(6 intermediate revisions by the same user not shown)
Line 51: Line 51:
 
|-
 
|-
 
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
 
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: CA8000
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''dsbC<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''dsbC''::Tn''10''
 +
|
 +
*Reference Condition:
 +
|
 +
 +
|
 +
 +
|
 +
0000178
 +
|
 +
in vivo assay evidence
 +
|
 +
Sensitivity to DTT
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: CA8000
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''dsbC<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''dsbC''::Tn''10''
 +
|
 +
*Reference Condition:
 +
|
 +
 +
|
 +
 +
|
 +
0000178
 +
|
 +
in vivo assay evidence
 +
|
 +
Reduced alkaline phosphatase activity
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: CA8000
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''dsbC<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''dsbC''::Tn''10''
 +
|
 +
*Reference Condition:
 +
|
 +
 +
|
 +
 +
|
 +
0000178
 +
|
 +
in vivo assay evidence
 +
|
 +
Hypersensitivity to benzylpenicillin
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: CA8000
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''dsbC<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''dsbC''::Tn''10''
 +
|
 +
*Reference Condition:
 +
|
 +
 +
|
 +
 +
|
 +
0000178
 +
|
 +
in vivo assay evidence
 +
|
 +
Inability to grow on minimal medium
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: CA8000
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''dsbC<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''dsbC''::Tn''10''
 +
|
 +
*Reference Condition:
 +
|
 +
 +
|
 +
 +
|
 +
0000178
 +
|
 +
in vivo assay evidence
 +
|
 +
Inability to grow on minimal medium
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: C800
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''dsbC<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''dsbC13''::Tn''10''
 +
|
 +
*Reference Condition:
 +
|
 +
 +
|
 +
 +
|
 +
0000178
 +
|
 +
in vivo assay evidence
 +
|
 +
Defective Disulfude Bond formation. See figure 2 for experimental results.
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: C800
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''dsbA<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''dsbA101''::Tn''10''
 +
|
 +
*Reference Condition:
 +
|
 +
 +
|
 +
 +
|
 +
0000178
 +
|
 +
in vivo assay evidence
 +
|
 +
Defective Disulfude Bond formation. See figure 2 for experimental results.
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: C800
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''dsbB<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''dsbB65''::Tn''10''
 +
|
 +
*Reference Condition:
 +
|
 +
 +
|
 +
 +
|
 +
0000178
 +
|
 +
in vivo assay evidence
 +
|
 +
Defective Disulfude Bond formation. See figure 2 for experimental results.
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: CA8000
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''dsbB<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''dsbB''65::Tn''10''
 +
|
 +
*Reference Condition:
 +
|
 +
 +
|
 +
 +
|
 +
0000178
 +
|
 +
in vivo assay evidence
 +
|
 +
The strain possess primarily reduced ''dsbA'' protein.
 +
|
 +
  
 
|- class="tableEdit_footer"  
 
|- class="tableEdit_footer"  
Line 65: Line 290:
  
 
[[Category:Publication]]
 
[[Category:Publication]]
 +
[[Category:LaRossa citations that need review]]

Latest revision as of 12:35, 16 May 2013

Citation

Missiakas, D, Georgopoulos, C and Raina, S (1994) The Escherichia coli dsbC (xprA) gene encodes a periplasmic protein involved in disulfide bond formation. EMBO J. 13:2013-20

Abstract

We have identified and functionally characterized a new Escherichia coli gene, dsbC, whose product is involved in disulfide bond formation in the periplasmic space. It corresponds to a previously sequenced open reading frame mapping upstream of recJ with no previously assigned function. Null mutations in dsbC were obtained using a screen for dithiothreitol (DTT)-sensitive mutants and were shown to result in the accumulation of reduced forms of a variety of disulfide bond-containing periplasmic proteins. This defect could be rescued by the addition of either oxidized DTT or cystine or by multicopy expression of dsbA, a known periplasmic disulfide oxidase. The DsbC protein is synthesized as a precursor form of 25.5 kDa which is processed to a 23.3 kDa mature species located in the periplasmic space. The DsbC protein was overexpressed, purified to homogeneity and shown to catalyse the reduction of insulin in a DTT-dependent manner at levels comparable with those of purified DsbA. The replacement of either cysteine residue of the predicted active site, F-(X4)-C-G-Y-C, completely inactivates DsbC protein function. We have further shown that in vivo overexpression of DsbC can functionally substitute for a loss of DsbA function. Taken together, all of our results demonstrate that DsbC acts in vivo as a disulfide oxidase.

Links

PubMed PMC395044

Keywords

Amino Acid Sequence; Base Sequence; Chromosome Mapping; Cloning, Molecular; DNA Mutational Analysis; Disulfides/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial/genetics; Isomerases/genetics; Isomerases/metabolism; Membrane Proteins/genetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Disulfide Reductase (Glutathione)/genetics; Protein Disulfide Reductase (Glutathione)/metabolism; Protein Disulfide-Isomerases; Sequence Analysis, DNA; Sequence Homology, Amino Acid

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: CA8000
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: dsbC+
  • Genotype of Experimental Strain : dsbC::Tn10
  • Reference Condition:

0000178

in vivo assay evidence

Sensitivity to DTT

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: CA8000
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: dsbC+
  • Genotype of Experimental Strain : dsbC::Tn10
  • Reference Condition:

0000178

in vivo assay evidence

Reduced alkaline phosphatase activity

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: CA8000
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: dsbC+
  • Genotype of Experimental Strain : dsbC::Tn10
  • Reference Condition:

0000178

in vivo assay evidence

Hypersensitivity to benzylpenicillin

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: CA8000
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: dsbC+
  • Genotype of Experimental Strain : dsbC::Tn10
  • Reference Condition:

0000178

in vivo assay evidence

Inability to grow on minimal medium

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: CA8000
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: dsbC+
  • Genotype of Experimental Strain : dsbC::Tn10
  • Reference Condition:

0000178

in vivo assay evidence

Inability to grow on minimal medium

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: C800
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: dsbC+
  • Genotype of Experimental Strain : dsbC13::Tn10
  • Reference Condition:

0000178

in vivo assay evidence

Defective Disulfude Bond formation. See figure 2 for experimental results.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: C800
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: dsbA+
  • Genotype of Experimental Strain : dsbA101::Tn10
  • Reference Condition:

0000178

in vivo assay evidence

Defective Disulfude Bond formation. See figure 2 for experimental results.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: C800
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: dsbB+
  • Genotype of Experimental Strain : dsbB65::Tn10
  • Reference Condition:

0000178

in vivo assay evidence

Defective Disulfude Bond formation. See figure 2 for experimental results.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: CA8000
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: dsbB+
  • Genotype of Experimental Strain : dsbB65::Tn10
  • Reference Condition:

0000178

in vivo assay evidence

The strain possess primarily reduced dsbA protein.


edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

Retrieved from "http://bcbp-ftq4xm2.biobio.tamu.edu/omp/dev/index.php?title=PMID:8168498&oldid=10447"