Difference between revisions of "PMID:14153532"

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'''WITKIN, EM and SICURELLA, NA'''  (1964) PURE CLONES OF LACTOSE-NEGATIVE MUTANTS OBTAINED IN ESCHERICHIA COLI AFTER TREATMENT WITH 5-BROMOURACIL. ''J. Mol. Biol.'' '''8''':610-3
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!align=left  |Abstract
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No abstract in PubMed
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!align=left  |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14153532 PubMed]
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!align=left  |Keywords
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==Main Points of the Paper ==
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{{LitSignificance}}
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*Describes the introduction of mutations (by incorporation of 5BU into DNA) that results in cells unable to ferment lactose
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*Sectoring phenotypes appear, indicative of the timing the mutation gets incorporated within a cell of a colony
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== Materials and Methods Used ==
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{{LitMaterials}}
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*Parent strain: Z-15 (radiation-resistant, serine-requiring variant of the thymineless strain B3)
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*[[:Category:5-Bromouracil Mutagenesis|5-Bromouracil Mutagenesis]] (on plates)
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**strain plated on 5BU completed 1 round of replication, whereby most of the thymine was replaced by 5BU
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**strain plated on thymine (control) completed a normal round of DNA replication
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*Bacto-eosin-methylene blue (EMB) indicator agar (for more information, click [http://en.wikipedia.org/wiki/Eosin_methylene_blue here])
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**dark red colonies (lactose +)
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**colorless colonies (lactose -)
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**mixed
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***large sectors (lac- component originated at the center and constituted one-eighth or more the area of the colony)
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***peripheral sectors
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==Phenotype Annotations==
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{{AnnotationTableHelp}}
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="N517068576895e"  class=" tableEdit Phenotype_Table_2" 
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: z-15
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&p=mapview&p=has_linkout&p=blast_url&p=genome_blast&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: Strain z-15
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*Genotype of Experimental Strain : Derivatives of Z-15
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*Reference Condition:
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OMP:0000284
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loss of utilization of carbon-containing compound
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ECO:0000178
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in vivo assay evidence
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Abolished utilization of carbon source lactose in mutant strain. See Table 1.
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==Notes==
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OMP: After carbon utilization terms get finialized, make sure OMP ID matches what is on here since it might change.
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ECO: Terms for assays are awaiting implementation of solid and liquid child terms to each one. Experiment used EMB
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==References==
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{{RefHelp}}
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<references/>
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[[Category:Publication]]
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[[Category:5-Bromouracil Mutagenesis]]
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[[Category:LaRossa citations that need review]]

Latest revision as of 12:48, 19 April 2013

Citation

WITKIN, EM and SICURELLA, NA (1964) PURE CLONES OF LACTOSE-NEGATIVE MUTANTS OBTAINED IN ESCHERICHIA COLI AFTER TREATMENT WITH 5-BROMOURACIL. J. Mol. Biol. 8:610-3

Abstract

No abstract in PubMed

Links

PubMed

Keywords


edit table

Main Points of the Paper

Please summarize the main points of the paper.

  • Describes the introduction of mutations (by incorporation of 5BU into DNA) that results in cells unable to ferment lactose
  • Sectoring phenotypes appear, indicative of the timing the mutation gets incorporated within a cell of a colony

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

  • Parent strain: Z-15 (radiation-resistant, serine-requiring variant of the thymineless strain B3)
  • 5-Bromouracil Mutagenesis (on plates)
    • strain plated on 5BU completed 1 round of replication, whereby most of the thymine was replaced by 5BU
    • strain plated on thymine (control) completed a normal round of DNA replication
  • Bacto-eosin-methylene blue (EMB) indicator agar (for more information, click here)
    • dark red colonies (lactose +)
    • colorless colonies (lactose -)
    • mixed
      • large sectors (lac- component originated at the center and constituted one-eighth or more the area of the colony)
      • peripheral sectors

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: z-15
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: Strain z-15
  • Genotype of Experimental Strain : Derivatives of Z-15
  • Reference Condition:

OMP:0000284

loss of utilization of carbon-containing compound

ECO:0000178

in vivo assay evidence

Abolished utilization of carbon source lactose in mutant strain. See Table 1.


edit table

</protect>

Notes

OMP: After carbon utilization terms get finialized, make sure OMP ID matches what is on here since it might change.

ECO: Terms for assays are awaiting implementation of solid and liquid child terms to each one. Experiment used EMB

References

See Help:References for how to manage references in omp dev.

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